Molecular dynamics at Trypanosoma cruzi nucleus. / Dinâmica molecular no núcleo de Trypanosoma cruzi.
AUTOR(ES)
Simone Guedes Calderano
DATA DE PUBLICAÇÃO
2008
RESUMO
In Trypanosoma cruzi the replication sites are located at nuclear periphery. In order to analyse the dynamics of molecules involved in replication, Orc/Cdc6 was used as a marker of pre-replication machinery and PCNA as a marker of replication machinery during the cell cycle of epimastigote form. Both molecules presented two nuclear patterns: dispersed pattern and peripheral pattern. Double-labeling assay showed three different patterns of Orc/Cdc6 and PCNA in the nucleus: Orc/Cdc6 at nuclear periphery and PCNA dispersed, both dispersed and both at nuclear periphery. This data allowed us to conclude that during early G1 phase both molecules are dispersed in the nucleus and during late G1 Orc/Cdc6 goes to nuclear periphery while TcPCNA remains dispersed, moving to nuclear periphery, where DNA replication will take place, when S phase starts. Thus, the replication of DNA at nuclear periphery is not due to localization of replications factors at nuclear periphery; instead it depends on the movement of these factors to the appropriated sites.
ASSUNTO(S)
replication sites sítios de replicação orc trypanossoma cruzi replicação organização nuclear replication nuclear organization pcna trypanossoma cruzi pcna orc
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