Nla
Mostrando 13-24 de 35 artigos, teses e dissertações.
-
13. Investigation of Mutations in Loco G6PD (Bmed, A+A376G, A-G202A) in two Locality of Porto Velho-RO. / INVESTIGAÇÃO DE MUTAÇÕES NO LOCO G6PD (B, A+A376G, A-G202A, BMed) EM DUAS LOCALIDADES DE PORTO VELHO - RO
G6PD (Xq28), glucolysis enzyme of the metabolic Warburg-Dickens, is responsible in keeping the level of reduced NADPH of the erythrocyte cells. The enzymatic deficiency of some variants affects about 400 million people in the worldwide. In Brazil, the frequency of mutants is around 1,6%; as in generality observed on neonatal "screening" in the Brazilian popu
Publicado em: 2005
-
14. Investigação genético-epidemiológica e molecular da deficiência de G-6-PD em uma comunidade brasileira
Este trabalho teve por objetivo estudar a deficiência de G-6-PD em uma comunidade do interior do Estado de São Paulo (Bragança Paulista). Durante 36 meses foram selecionados 4.621 doadores de sangue do sexo masculino, detectando-se 80 deficientes em G-6-PD. A análise molecular foi realizada em 70 deficientes não consangüíneos mediante a amplificação
Cadernos de Saúde Pública. Publicado em: 2000-06
-
15. Influencia do anel B na estabilidade das antocianidinas antocianinas
Os extratos metanólicos da berinjela, amora e morango foram fracionados e as principais antocianinas das frações foram identificadas e usadas para estudar sua estabilidade. Cada aniocianína foi identificada por cromatografia em papel, características espectrocópicas e reações químicas especificas, A estabilidade de cada antocianina e sua agi i cona,
Publicado em: 1994
-
16. Global Mutational Analysis of NtrC-Like Activators in Myxococcus xanthus: Identifying Activator Mutants Defective for Motility and Fruiting Body Development
The multicellular developmental cycle of Myxococcus xanthus requires large-scale changes in gene transcription, and recent findings indicate that NtrC-like activators play a prominent role in regulating these changes. In this study, we made insertions in 28 uncharacterized ntrC-like activator (nla) genes and found that eight of these insertions cause develop
American Society for Microbiology.
-
17. Functional analysis of iceA1, a CATG-recognizing restriction endonuclease gene in Helicobacter pylori
iceA1 in Helicobacter pylori is a homolog of nlaIIIR, which encodes the CATG-specific restriction endonuclease NlaIII in Neisseria lactamica. Analysis of iceA1 sequences from 49 H.pylori strains shows that a full-length NlaIII-like ORF is present in 10 strains, including CH4, but in other strains, including strain 60190, the ORFs are truncated due to a varie
Oxford University Press.
-
18. Nuclear transport of plant potyviral proteins.
We have used immunoblotting, immunocytochemical, and gene fusion methods to examine the differential subcellular partitioning of tobacco etch potyvirus proteins that are potentially associated with RNA replication. From the earliest timepoints at which viral proteins could be detected, proteins Nla (49-kilodalton proteinase) and Nlb (58-kilodalton polymerase
-
19. The Helicobacter pylori genome is modified at CATG by the product of hpyIM.
To understand mechanisms of DNA methylation in Helicobacter pylori, a human pathogen associated with peptic ulcer disease and gastric adenocarcinoma, we cloned a putative DNA methyltransferase gene, hpyIM. This gene contains a 990-bp open reading frame encoding a 329-amino-acid protein, M.HpyI. Sequence analysis revealed that M.HpyI was closely related to CA
-
20. A NlaIV polymorphism within the human Factor X gene
-
21. Endothelium-dependent hyperpolarization caused by bradykinin in human coronary arteries.
The present study was designed to determine whether bradykinin induces endothelium-dependent hyperpolarization of vascular smooth muscle in human coronary arteries, and if so, to define the contribution of this hyperpolarization to endothelium-dependent relaxations. The membrane potential of arterial smooth muscle cells (measured by glass microelectrodes) an
-
22. Improved NlaIII digestion of PAGE-purified 102 bp ditags by addition of a single purification step in both the SAGE and microSAGE protocols
Despite the success of microarray technologies, serial analysis of gene expression (SAGE) still remains the only technique that allows an accurate quantitative and qualitative analysis of cell transcription in a variety of physiological and pathological conditions. Nevertheless, the efficiency of SAGE is limited by the numerous gel purification steps require
Oxford University Press.
-
23. Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system.
A second DNA site-specific (restriction) endonuclease (R.CviAII) and its cognate adenine DNA methyltransferase (M.CviAII) were isolated from virus PBCV-1 infected Chlorella strain NC64A cells. R.CviAII, a heteroschizomer of the bacterial restriction endonuclease NlaIII, recognizes the sequence CATG, and does not cleave CmATG sequences. However, unlike NlaIII
-
24. Assessment of SAGE in Transcript Identification
An essential step in Serial Analysis of Gene Expression (SAGE) is tag mapping, which refers to the unambiguous determination of the gene represented by a SAGE tag. Current resources for tag mapping are incomplete, and thus do not allow assessment of the efficacy of SAGE in transcript identification. A method of tag mapping is described here and applied t
Cold Spring Harbor Laboratory Press.