Mycobacterium Smegmatis And Mycobacterium Tuberculosis
Mostrando 1-12 de 192 artigos, teses e dissertações.
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1. DITERPENOS ENT-ABIETANOS DE Euphorbia phosphorea (EUPHORBIACEAE)
Phytochemical study of the roots of Euphorbia phosphorea Mart. (Euphorbiaceae) was carried out through chromatographic techniques, resulting in the isolation of a new ent-abietane diterpene named 11β,12β-dihydroxy-ent-abieta-8(14),13(15)-dien-16,12α-olide (1), and of nine known ent-abietane diterpenes jolkinolide A (2), jolkinolide E (3), euphorin H (4),
Química Nova. Publicado em: 2022
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2. Influence of iron deprivation on virulence traits of mycobacteria
ABSTRACT Novel strategies to combat the ever increasing burden of drug resistance in Mycobacterium tuberculosis (MTB) causing tuberculosis (TB) remains a global concern. The ability of MTB to sense and adapt to restricted iron conditions in the hostile environment is essential for their survival and confers the basis of their success as dreadful pathogen. Th
Braz J Infect Dis. Publicado em: 2016-12
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3. Antimycobacterial activity of the fractions and compounds from Scutia buxifolia
The antimycobacterial activity of Scutia buxifolia Reissek, Rhamnaceae, leaves extracts and fractions were evaluated for the first time. Four compounds were identified, flavonoids (quercetin and quercitrin) and phenolic acids (gallic and caffeic acids) and quantified by HPLC-DAD. Promising anti-Mycobacterium smegmatis activity was observed with ethyl acetate
Revista Brasileira de Farmacognosia. Publicado em: 07/10/2011
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4. SÃntese e avaliaÃÃo da atividade antimicrobiana de derivados 4-Tiazolidinonas: anÃlogos estruturais piridÃnicos
As aÃÃes terapÃuticas de alguns fÃrmacos podem ser melhoradas atravÃs da modificaÃÃo molecular. SÃo conhecidas as propriedades biolÃgicas de numerosos compostos heterocÃclicos, entre os quais aqueles contendo enxofre e nitrogÃnio no seu anel. Entre estes compostos os derivados, das 4-tiazolidinonas merecem destaque especial por suas atividades ant
Publicado em: 2008
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5. Construction of auxotrophic marker in Mycobacterium bovis BCG, knockout strain for the DPPD and proteomic study of tuberculin / Construção de marcador auxotrófico em Mycobacterium bovis BCG, de uma cepa knockout para DPPD e estudo proteômico da tuberculina
Mycobacterium bovis BCG has the potential to be an effective live vector for multivalent vaccines. However, there are two problems regarding the utilization of recombinant BCG as vaccine. The first one is that most mycobacterial cloning vectors rely on antibiotic resistance gene as selectable marker, which is used for genetic transformation. The second one i
Publicado em: 2008
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6. SÃntese e avaliaÃÃo do potencial biolÃgico de novos derivados N-fenacilados do aza-biciclo pirrolidino [3,2-d] isoxazolina
Recently, our group of research had been gotten N-(benzoyl)-pyrrolidine[3,2-d]-2- isoxazolines derivatives 40, by 1,3-dipolar cycloaddition of five membered endociclic enecarbamates and enamides, with excellent biological activities. Due to this fact, we intended the obtention of the derivatives N-(fenacyl)-pyrrolidine[3,2-d]-2-isoxazolines 41, using the aza
Publicado em: 2005
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7. Evidence for the expression of native Mycobacterium tuberculosis phospholipase C: recognition by immune sera and detection of promoter activity
The genome of Mycobacterium tuberculosis H37Rv contains three contiguous genes (plc-a, plc-b and plc-c) which are similar to the Pseudomonas aeruginosa phospholipase C (PLC) genes. Expression of mycobacterial PLC-a and PLC-b in E. coli and M. smegmatis has been reported, whereas expression of the native proteins in M. tuberculosis H37Rv has not been demonstr
Brazilian Journal of Medical and Biological Research. Publicado em: 2000-11
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8. Cloning and Characterization of Arylamine N-Acetyltransferase Genes from Mycobacterium smegmatis and Mycobacterium tuberculosis: Increased Expression Results in Isoniazid Resistance
Arylamine N-acetyltransferases (NATs) are found in many eukaryotic organisms, including humans, and have previously been identified in the prokaryote Salmonella typhimurium. NATs from many sources acetylate the antitubercular drug isoniazid and so inactivate it. nat genes were cloned from Mycobacterium smegmatis and Mycobacterium tuberculosis, and expressed
American Society for Microbiology.
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9. Characterization of Mycobacterium smegmatis Expressing the Mycobacterium tuberculosis Fatty Acid Synthase I (fas1) Gene
Unlike most other bacteria, mycobacteria make fatty acids with the multidomain enzyme eukaryote-like fatty acid synthase I (FASI). Previous studies have demonstrated that the tuberculosis drug pyrazinamide and 5-chloro-pyrazinamide target FASI activity. Biochemical studies have revealed that in addition to C16:0, Mycobacterium tuberculosis FASI synthesizes C
American Society for Microbiology.
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10. A Protein Secretion Pathway Critical for Mycobacterium tuberculosis Virulence Is Conserved and Functional in Mycobacterium smegmatis
The Snm protein secretion system is a critical determinant of Mycobacterium tuberculosis virulence. However, genes encoding components of this pathway are conserved among all mycobacteria, including the nonpathogenic saprophyte Mycobacterium smegmatis. We show that the Snm system is operational in M. smegmatis and that secretion of its homologous ESAT-6 and
American Society for Microbiology.
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11. Mycobacterium smegmatis strain for detection of Mycobacterium tuberculosis by PCR used as internal control for inhibition of amplification and for quantification of bacteria.
For the detection of Mycobacterium tuberculosis by PCR, the IS6110 sequence was used. A modified target was constructed by insertion of 56 nucleotides in the IS6110 insertion element of Mycobacterium bovis BCG. This modified insertion sequence was integrated into the genome of Mycobacterium smegmatis, a mycobacterium species which does not contain the IS6110
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12. Polyprenyl Phosphate Biosynthesis in Mycobacterium tuberculosis and Mycobacterium smegmatis
Mycobacterium smegmatis has been shown to contain two forms of polyprenyl phosphate (Pol-P), while Mycobacterium tuberculosis contains only one. Utilizing subcellular fractions from M. smegmatis and M. tuberculosis, we show that Pol-P synthesis is different in these species. The specific activities of the prenyl diphosphate synthases in M. tuberculosis are 1
American Society for Microbiology.