Mpb70
Mostrando 13-24 de 38 artigos, teses e dissertações.
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13. Identification of Mycobacterium bovis antigens by analysis of bovine T-cell responses after infection with a virulent strain
Purification and characterization of individual antigenic proteins are essential for the understanding of the pathogenic mechanisms of mycobacteria and the immune response against them. In the present study, we used anion-exchange chromatography to fractionate cell extracts and culture supernatant proteins from Mycobacterium bovis to identify T-cell-stimulat
Brazilian Journal of Medical and Biological Research. Publicado em: 2003-11
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14. Hoje preciso refletir um pouco: ser social e tempo histórico na obra de Chico Buarque de Hollanda 1971/1978
Este artigo analisa a relação entre o ser social e o tempo histórico em cinco canções compostas por Chico Buarque de Hollanda durante os anos 70, em plena vigência do regime militar no Brasil. Nossa perspectiva é a de que a obra deste compositor revela uma singular articulação entre aquelas duas categorias, expressando várias formas de consciência
História (São Paulo). Publicado em: 2003
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15. Nested-PCR usando o fragmento MPB64 melhora o diagnóstico da tuberculose pleural e meníngea
O Mycobacterium tuberculosis é raramente encontrado em fluidos como o líquido pleural e o cérebroespinhal, tornando estas localizações de difícil diagnóstico. Apresentamos nossa experiência com uma técnica de PCR aplicada a líquido pleural e cerebroespinhal com o uso do primer MPB64. Sessenta e três espécimes foram analisados: 30 líquidos pleura
Revista da Sociedade Brasileira de Medicina Tropical. Publicado em: 2000-06
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16. Industria fonografica : relações sociais de produção e concepção acerca da natureza do trabalho artistico, um estudo antropologico: a industria do disco no Brasil e a imagem publica de dois compositores-interpretes de MPB na decada de 70
Not informed
Publicado em: 1988
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17. Properties of proteins MPB64, MPB70, and MPB80 of Mycobacterium bovis BCG.
The immunogenic proteins MPB64 and MPB80 of Mycobacterium bovis BCG were purified to homogeneity and compared with MPB70. MPB70 and MPB80 showed a similar distribution in substrains of BCG, both being present in high concentrations in culture fluids of BCG substrain Tokyo, BCG Moreau, BCG Russia, and BCG Sweden and in only very small amounts in BCG Glaxo, BC
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18. Immunochemical Characterization of the MPB70/80 and MPB83 Proteins of Mycobacterium bovis
MPB70 and MPB80 (MPB70/80) and MPB83 are closely related antigens which are highly expressed in Mycobacterium bovis. MPB70/80 are soluble secreted antigens, while MPB83 is an exported lipoprotein associated with the bacterial surface. In the present study, these antigens had different mobilities in sodium dodecyl sulfate-polyacrylamide gel electrophoresis un
American Society for Microbiology.
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19. Synthetic Peptides Identify Promiscuous Human Th1 Cell Epitopes of the Secreted Mycobacterial Antigen MPB70
MPB70 is a secreted protein of Mycobacterium bovis and Mycobacterium tuberculosis which stimulates both cellular and humoral immune responses during infection with bovine and human tubercle bacilli. In addition, vaccination with MPB70 has been shown to induce Th1 cell responses and protection in animal models of tuberculosis. The present study was carried ou
American Society for Microbiology.
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20. Protein G-based enzyme-linked immunosorbent assay for anti-MPB70 antibodies in bovine tuberculosis.
MPB70 is a highly species specific protein which is secreted from Mycobacterium bovis during culture. To investigate whether antibodies against MPB70 can be used as an indicator of infection with M. bovis, an enzyme-linked immunosorbent assay was developed, based on the use of biotinylated protein G, to provide a common indicator for antibody formation in di
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21. Modification of the Mycobacterium bovis extracellular protein MPB70 with fluorescein for rapid detection of specific serum antibodies by fluorescence polarization.
The principle of fluorescence polarization described by Perrin (F. Perrin, J. Phys. Radium 7:390-401, 1926) was applied to the development of a novel assay that used fluorescein-labeled Mycobacterium bovis secretory protein MPB70 for rapid detection of anti-MPB70 antibodies in selected sera from three M. bovis-infected species (elk, Ilama, and bison). Labeli
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22. Relationship of secretion pattern and MPB70 homology with osteoblast-specific factor 2 to osteitis following Mycobacterium bovis BCG vaccination.
Significant homology was found between MPB70 and each of four repeat domains of osteoblast-specific factor 2 (OSF-2). Two internal homology regions within each repeat domain of OSF-2 presumed to be related to the active site(s) of this bone adhesion molecule showed the highest homology. A literature search concerning osteitis after Mycobacterium bovis BCG va
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23. Specific skin-reactive protein from culture filtrate of Mycobacterium bovis BCG.
A highly purified protein, named MPB70, was isolated from the culture filtrate of Mycobacterium bovis BCG. This protein accounted for more than 10% of the proteins secreted into the culture medium. MPB70 was purified by precipitation with ammonium sulfate, followed by treatment with diethylaminoethyl ion exchanger, with or without 3 M urea, and by gel filtra
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24. Comparative studies with various substrains of Mycobacterium bovis BCG on the production of an antigenic protein, MPB70.
A protein, isolated and purified from the unheated culture filtrate of Mycobacterium bovis BCG (substrain Tokyo 172) and designated MPB70, elicited a delayed skin reaction in guinea pigs sensitized with viable cells of BCG but not in those sensitized with heat-killed cells. The skin reaction reached the maximum 4 to 8 weeks after the inoculation of the BCG a