Molecular Motors
Mostrando 13-24 de 89 artigos, teses e dissertações.
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13. Molecular Cloning and Functional Analysis of Mouse C-Terminal Kinesin Motor KifC3
Proteins of the kinesin superfamily define a class of microtubule-dependent motors that play crucial roles in cell division and intracellular transport. To study the molecular mechanism of intracellular transport involving microtubule-dependent motors, a cDNA encoding a new kinesin-like protein called KifC3 was cloned from a mouse brain cDNA library. Sequenc
American Society for Microbiology.
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14. Review of Scholey, Motility Assays for Molecular Motors, in Methods in Cell Biology, vol. 39
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15. Monte Carlo modeling of single-molecule cytoplasmic dynein
Molecular motors are responsible for active transport and organization in the cell, underlying an enormous number of crucial biological processes. Dynein is more complicated in its structure and function than other motors. Recent experiments have found that, unlike other motors, dynein can take different size steps along microtubules depending on load and AT
National Academy of Sciences.
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16. Intracellular actin-based transport: How far you go depends on how often you switch
Intracellular molecular motor-driven transport is essential for such diverse processes as mitosis, neuronal function, and mitochondrial transport. Whereas there have been in vitro studies of how motors function at the single-molecule level, and in vivo studies of the structure of filamentary networks, studies of how the motors effectively use the networks fo
National Academy of Sciences.
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17. Myosin motors with artificial lever arms.
The myosin head consists of a globular catalytic domain and a light chain binding domain (LCBD). The coupling efficiency between ATP hydrolysis and myosin-induced actin movement is known to decline as the LCBD is truncated or destabilized. However, it was not clear whether the observed alteration in the production of force and movement reflects only the mech
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18. Kinesin-related proteins in the mammalian testes: candidate motors for meiosis and morphogenesis.
The kinesin superfamily of molecular motors comprises proteins that participate in a wide variety of motile events within the cell. Members of this family share a highly homologous head domain responsible for force generation attached to a divergent tail domain thought to couple the motor domain to its target cargo. Many kinesin-related proteins (KRPs) parti
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19. Structure of a genetically engineered molecular motor
Molecular motors move unidirectionally along polymer tracks, producing movement and force in an ATP-dependent fashion. They achieve this by amplifying small conformational changes in the nucleotide-binding region into force-generating movements of larger protein domains. We present the 2.8 Å resolution crystal structure of an artificial actin-based motor.
Oxford University Press.
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20. Bidirectional Translocation of Neurofilaments along Microtubules Mediated in Part by Dynein/DynactinV⃞
Neuronal cytoskeletal elements such as neurofilaments, F-actin, and microtubules are actively translocated by an as yet unidentified mechanism. This report describes a novel interaction between neurofilaments and microtubule motor proteins that mediates the translocation of neurofilaments along microtubules in vitro. Native neurofilaments purified from
The American Society for Cell Biology.
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21. Holding two heads together: Stability of the myosin II rod measured by resonance energy transfer between the heads
Myosin, similar to many molecular motors, is a two-headed dimer held together by a coiled-coiled rod. The stability of the coiled coil has implications for head–head interactions, force generation, and possibly regulation. Here we used two different resonance energy transfer techniques to measure the distances between probes placed in the regulatory light
The National Academy of Sciences.
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22. Multiple actin-based motor genes in Dictyostelium.
Dictyostelium cells, devoid of conventional myosin, display a variety of motile activities, consistent with the presence of other molecular motors. The Dictyostelium genome was probed at low stringency with a gene fragment containing the conserved conventional myosin head domain sequences to identify other actin-based motors that may play a role in the obser
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23. Facilitation of dendritic mRNA transport by CPEB
In neurons, the proteins derived from mRNAs localized in dendrites have been implicated in synaptic plasticity. The cytoplasmic polyadenylation element (CPE), a cis element in the 3′-UTRs of specific dendritic mRNAs, promotes cytoplasmic polyadenylation-induced translation in response to synaptic stimulation. Here, we demonstrate that the CPE and its bindi
Cold Spring Harbor Laboratory Press.
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24. Chemical peristalsis
Molecules that emulate in part the remarkable capabilities of protein motors were recently chemically synthesized. A promising approach is based on physically interlocked macromolecular complexes such as rotaxanes and catenanes. Using the latter, Leigh et al. [Leigh, D. A., Wong, J. K. Y., Dehez, F. & Zerbetto, F. (2003) Nature 424, 174–179] constructed a
National Academy of Sciences.