Intracellular actin-based transport: How far you go depends on how often you switch
AUTOR(ES)
Snider, Joseph
FONTE
National Academy of Sciences
RESUMO
Intracellular molecular motor-driven transport is essential for such diverse processes as mitosis, neuronal function, and mitochondrial transport. Whereas there have been in vitro studies of how motors function at the single-molecule level, and in vivo studies of the structure of filamentary networks, studies of how the motors effectively use the networks for transportation have been lacking. We investigate how the combined system of myosin-V motors plus actin filaments is used to transport pigment granules in Xenopus melanophores. Experimentally, we characterize both the actin filament network, and how this transport is altered in response to external signals. We then develop a theoretical formalism to explain these changes. We show that cells regulate transport by controlling how often granules switch from one filament to another, rather than by altering individual motor activity at the single-molecule level, or by relying on structural changes in the network.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=516548Documentos Relacionados
- Actin-Based Motility of Intracellular Microbial Pathogens
- Multiple actin-based motor genes in Dictyostelium.
- How far would you go?
- Volatile anesthetics block actin-based motility in dendritic spines
- Myosin Motors and Not Actin Comets Are Mediators of the Actin-based Golgi-to-Endoplasmic Reticulum Protein TransportD⃞