Molecular Motor Proteins
Mostrando 13-24 de 73 artigos, teses e dissertações.
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13. The shapes of the motor domains of two oppositely directed microtubule motors, ncd and kinesin: a neutron scattering study.
The shapes of the motor domains of kinesin and ncd, which move in opposite directions along microtubules, have been investigated. Using proteins expressed in Escherichia coli, it was found that at high salt (> 200 mM) Drosophila ncd motor domain (R335-K700) and human kinesin motor domain (M1-E349) were both sufficiently monomeric to allow an accurate determi
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14. JNK phosphorylation of Bim-related members of the Bcl2 family induces Bax-dependent apoptosis
The c-Jun NH2-terminal kinase (JNK) is activated when cells are exposed to environmental stress, including UV radiation. Gene disruption studies demonstrate that JNK is essential for UV-stimulated apoptosis mediated by the mitochondrial pathway by a Bax/Bak-dependent mechanism. Here, we demonstrate that JNK phosphorylates two members of the BH3-only subgroup
The National Academy of Sciences.
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15. Mechanochemical coupling of the motion of molecular motors to ATP hydrolysis.
The typical biochemical paradigm for coupling between hydrolysis of ATP and the performance of chemical or mechanical work involves a well-defined sequence of events (a kinetic mechanism) with a fixed stoichiometry between the number of ATP molecules hydrolyzed and the turnover of the output reaction. Recent experiments show, however, that such a determinist
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16. Crystal structure of Mycobacterium tuberculosis SecA, a preprotein translocating ATPase
In bacteria, the majority of exported proteins are translocated by the Sec system, which recognizes the signal sequence of a preprotein and uses ATP and the proton motive force to mediate protein translocation across the cytoplasmic membrane. SecA is an essential protein component of this system, containing the molecular motor that facilitates translocation.
The National Academy of Sciences.
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17. Bidirectional Translocation of Neurofilaments along Microtubules Mediated in Part by Dynein/DynactinV⃞
Neuronal cytoskeletal elements such as neurofilaments, F-actin, and microtubules are actively translocated by an as yet unidentified mechanism. This report describes a novel interaction between neurofilaments and microtubule motor proteins that mediates the translocation of neurofilaments along microtubules in vitro. Native neurofilaments purified from
The American Society for Cell Biology.
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18. Molecular Motor KIF1C Is Not Essential for Mouse Survival and Motor-Dependent Retrograde Golgi Apparatus-to-Endoplasmic Reticulum Transport
KIF1C is a new member of the kinesin superfamily of proteins (KIFs), which act as microtubule-based molecular motors involved in intracellular transport. We cloned full-length mouse kif1C cDNA, which turned out to have a high homology to a mitochondrial motor KIF1Bα and to be expressed ubiquitously. To investigate the in vivo significance of KIF1C, we gener
American Society for Microbiology.
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19. Molecular mechanisms of spindle function
The key molecules involved in regulating the assembly and function of the mitotic spindle are shared by evolutionarily divergent species. Studies in different model systems are leading to convergent conclusions about the central role of microtubule nucleation and dynamics and of kinesin-related motor proteins in spindle function.
BioMed Central.
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20. Detection of sub-8-nm movements of kinesin by high-resolution optical-trap microscopy.
Kinesin is a molecular motor that transports organelles along microtubules. This enzyme has two identical 7-nm-long motor domains, which it uses to move between consecutive tubulin binding sites spaced 8 nm apart along a microtubular protofilament. The molecular mechanism of this movement, which remains to be elucidated, may be common to all families of moto
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21. A GTP-driven motor moves proteins across the outer envelope of chloroplasts
The translocation of proteins across cellular membranes is a key mechanistic problem for every cell. The preprotein translocon at the chloroplast outer envelope is responsible for precursor protein recognition and translocation across the outer envelope. We have reconstituted the translocation process into proteoliposomes from single subunits or by using the
The National Academy of Sciences.
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22. Misfolded CuZnSOD and amyotrophic lateral sclerosis
Amyotrophic lateral sclerosis (ALS) is a progressive degenerative disease of motor neurons. The inherited form of the disease, familial ALS, represents 5–10% of the total cases, and the best documented of these are due to lesions in SOD1, the gene encoding copper–zinc superoxide dismutase (CuZnSOD). The mechanism by which mutations in SOD1 cause familial
The National Academy of Sciences.
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23. Molecular Cloning and Functional Analysis of Mouse C-Terminal Kinesin Motor KifC3
Proteins of the kinesin superfamily define a class of microtubule-dependent motors that play crucial roles in cell division and intracellular transport. To study the molecular mechanism of intracellular transport involving microtubule-dependent motors, a cDNA encoding a new kinesin-like protein called KifC3 was cloned from a mouse brain cDNA library. Sequenc
American Society for Microbiology.
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24. African Swine Fever Virus Protein p54 Interacts with the Microtubular Motor Complex through Direct Binding to Light-Chain Dynein
Dynein is a minus-end-directed microtubule-associated motor protein involved in cargo transport in the cytoplasm. African swine fever virus (ASFV), a large DNA virus, hijacks the microtubule motor complex cellular transport machinery during virus infection of the cell through direct binding of virus protein p54 to the light chain of cytoplasmic dynein (LC8).
American Society for Microbiology.