Microfabrication
Mostrando 25-36 de 36 artigos, teses e dissertações.
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25. Microfabricated Fountain Pens for High-Density DNA Arrays
We used photolithographic microfabrication techniques to create very small stainless steel fountain pens that were installed in place of conventional pens on a microarray spotter. Because of the small feature size produced by the microfabricated pens, we were able to print arrays with up to 25,000 spots/cm2, significantly higher than can be achieved by other
Cold Spring Harbor Laboratory Press.
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26. Guiding neuronal development with in situ microfabrication
We report the ability to modify microscopic 3D topographies within dissociated cultures, providing a means to alter the development of neurons as they extend neurites and establish interconnections. In this approach, multiphoton excitation is used to focally excite noncytotoxic photosensitizers that promote protein crosslinking, such as BSA, into matrices ha
National Academy of Sciences.
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27. Fabrication of aligned microstructures with a single elastomeric stamp
The fabrication of complex patterns of aligned microstructures has required the use of multiple applications of lithography. Here we describe an approach for microfabrication that encodes the two-dimensional spatial information of several photomasks onto a single elastomeric stamp by mapping each photomask onto distinct heights on the surface of the stamp. P
The National Academy of Sciences.
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28. Microfluidics without microfabrication
Microfluidic devices create spatially defined, chemically controlled environments at microscopic dimensions. We demonstrate the formation and control of microscopic hydrodynamic and chemical environments by impinging a low-intensity acoustic oscillation on a cylindrical electrode. The interaction of small-amplitude (≤203 μm), low-frequency (≤515 Hz) flu
The National Academy of Sciences.
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29. Assessing Neural Stem Cell Motility Using an Agarose Gel-based Microfluidic Device
While microfluidic technology is reaching a new level of maturity for macromolecular assays, cell-based assays are still at an infant stage1. This is largely due to the difficulty with which one can create a cell-compatible and steady microenvironment using conventional microfabrication techniques and materials. We address this problem via the introduction
MyJove Corporation.
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30. Microassembly based on hands free origami with bidirectional curvature
Microassembly based on origami, the Japanese art of paper folding, presents an attractive methodology for constructing complex three-dimensional (3D) devices and advanced materials. A variety of functional structures have been created using patterned metallic, semiconducting, and polymeric thin films, but have been limited to those that curve in a single dir
American Institute of Physics.
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31. Microfabricated needles for transdermal delivery of macromolecules and nanoparticles: Fabrication methods and transport studies
Arrays of micrometer-scale needles could be used to deliver drugs, proteins, and particles across skin in a minimally invasive manner. We therefore developed microfabrication techniques for silicon, metal, and biodegradable polymer microneedle arrays having solid and hollow bores with tapered and beveled tips and feature sizes from 1 to 1,000 μm. When solid
National Academy of Sciences.
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32. Emergent patterns of growth controlled by multicellular form and mechanics
Spatial patterns of cellular growth generate mechanical stresses that help to push, fold, expand, and deform tissues into their specific forms. Genetic factors are thought to specify patterns of growth and other behaviors to drive morphogenesis. Here, we show that tissue form itself can feed back to regulate patterns of proliferation. Using microfabrication
National Academy of Sciences.
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33. Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays
A chemical procedure was developed to functionalize poly(methyl methacrylate) (PMMA) substrates. PMMA is reacted with hexamethylene diamine to yield an aminated surface for immobilizing DNA in microarrays. The density of primary NH2 groups was 0.29 nmol/cm2. The availability of these primary amines was confirmed by the immobilization of DNA probes and hybrid
Oxford University Press.
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34. Microengineered hydromechanical cochlear model
Micromachined fluid-filled variable impedance waveguides intended to mimic the mechanics of the passive mammalian cochlea have been fabricated and experimentally examined. The structures were microfabricated with dimensions similar to those of the biological system. Experimental tests demonstrate acoustically excited traveling fluid-structure waves with phas
National Academy of Sciences.
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35. Microcontact Printing of Proteins for Cell Biology
The ability to pattern proteins and other biomolecules onto substrates is important for capturing the spatial complexity of the extracellular environment. Development of microcontact printing by the Whitesides group (http://gmwgroup.harvard.edu/) in the mid-1990s revolutionalized this field by making microelectronics/microfabrication techniques accessible t
MyJove Corporation.
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36. Paper-supported 3D cell culture for tissue-based bioassays
Fundamental investigations of human biology, and the development of therapeutics, commonly rely on 2D cell-culture systems that do not accurately recapitulate the structure, function, or physiology of living tissues. Systems for 3D cultures exist but do not replicate the spatial distributions of oxygen, metabolites, and signaling molecules found in tissues.
National Academy of Sciences.