Functionalization of poly(methyl methacrylate) (PMMA) as a substrate for DNA microarrays
AUTOR(ES)
Fixe, F.
FONTE
Oxford University Press
RESUMO
A chemical procedure was developed to functionalize poly(methyl methacrylate) (PMMA) substrates. PMMA is reacted with hexamethylene diamine to yield an aminated surface for immobilizing DNA in microarrays. The density of primary NH2 groups was 0.29 nmol/cm2. The availability of these primary amines was confirmed by the immobilization of DNA probes and hybridization with a complementary DNA strand. The hybridization signal and the hybridization efficiency of the chemically aminated PMMA slides were comparable to the hybridization signal and the hybridization efficiency obtained from differently chemically modified PMMA slides, silanized glass, commercial silylated glass and commercial plastic Euray™ slides. Immobilized and hybridized densities of 10 and 0.75 pmol/cm2, respectively, were observed for microarrays on chemically aminated PMMA. The immobilized probes were heat stable since the hybridization performance of microarrays subjected to 20 PCR heat cycles was only reduced by 4%. In conclusion, this new strategy to modify PMMA provides a robust procedure to immobilize DNA, which is a very useful substrate for fabricating single use diagnostics devices with integrated functions, like sample preparation, treatment and detection using microfabrication and microelectronic techniques.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=373302Documentos Relacionados
- A Microrheological Study of Poly(Methyl Methacrylate) Elastomer/Poly(Ethylene Terephthalate) (PMMAelast/PET) Blends
- Effects of nystatin, fluconazole and propolis on poly(methyl methacrylate) resin surface
- Cytotoxicity Assessment of a Poly(methyl methacrylate) Synthesized for the Direct Fabrication of Bone Tissues
- A comparison of 141 polymacon (Iogel) and 140 poly(methyl methacrylate) intraocular lens implants.
- Synthesis and photophysical properties of a poly(methyl methacrylate) polymer with carbazolyl side groups