Lytic Enzyme
Mostrando 1-12 de 207 artigos, teses e dissertações.
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1. Determination of lytic enzyme activities of indigenous Trichoderma isolates from Pakistan
Abstract This study investigated lytic enzyme activities in three indigenous Trichoderma strains namely, Trichoderma asperellum, Trichoderma harzianum and Trichoderma sp. Native Trichoderma strains and a virulent strain of Rhizoctonia solani isolated from infected bean plants were also included in the study. Enzyme activities were determined by measuring sug
Braz. J. Microbiol.. Publicado em: 09/10/2015
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2. Evaluation of a chemiluminescent enzyme-linked immunosorbent assay for the diagnosis of Trypanosoma cruzi infection in a nonendemic setting
The disappearance of lytic, protective antibodies (Abs) from the serum of patients with Chagas disease is accepted as a reliable indicator of parasitological cure. The efficiency of a chemiluminescent enzyme-linked immunosorbent assay based on a purified, trypomastigote-derived glycosylphosphatidylinositol-anchored mucin antigen for the serologic detection o
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-11
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3. Identificação e expressão de genes da biossíntese do jasmonato na interação entre Theobroma cacao e Moniliophthora perniciosa / Identification and expression of genes associated with jasmonate biosynthesis in the Theobroma cacao and Moniliophthora perniciosa interaction
Witches broom disease of cacao (Theobroma cacao L.), caused by the basidiomycete Moniliophthora perniciosa is an important disease and the use of resistant varieties is the only economic and environmental long-term solution. Plant hormones are essential in the signaling network involved in the response against a variety of biotic and abiotic stresses. It is
Publicado em: 2009
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4. Atividade de plasmina simile e sequencia parcial de enzima fibrino(geno)litica do veneno de Bothrops lanceolatus (Fer de lance)
The main symptoms following bothropic envenoming are necroses, hemorrhage and coagulopathies, originating a characteristic and complex phisiopathology. The work of Lôbo de Araújo et al. (1998) described a single polipeptide chain protein (PII-1A) purified from Bothrops lanceolatus venom, a snake inhabitant of the Martinica island. The immunodiffusion and i
Publicado em: 2005
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5. Production, purification and characterization of the enzyme B-1, 3 glucanase from Cellulomonas cellulans YLM-B191-1 and action of the enzyme in the cell wall of yeasts / Produção, purificação e caracterização da enzima [beta]-1,3-glucanase de Cellulomonas cellulans YLM-B191-1 e ação da enzima na parede celular de leveduras
The objective of this research was to isolate microorganisms which produced yeast cell walllytic enzymes and to study the production, purification and characterization of a Iytic ~-1,3-glucanase. The yeast-Iytic bacterium was isolated from the sludge of the Santa Helena sugar and alcohol factory in Piracicaba, SP. The isolated yeast-Iytic bacterium adhered t
Publicado em: 2002
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6. Purification and Properties of a Thermophilic Bacteriophage Lytic Enzyme 1
A phage lytic enzyme was isolated from lysates of Bacillus stearothermophilus (NCA 1503-4R). The enzyme was purified 1,998-fold with a 27% recovery of enzyme activity. By use of polyacrylamide gel electrophoresis and sucrose gradient centrifugation the enzyme was judged free from protein contaminants. The lytic enzyme was active over a pH range of 6.0 to 7.0
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7. Observations on the Group C Streptococcal Bacteriophage and Lytic Enzyme System1
Fox, Eugene N. (La Rabida-University of Chicago Institute, Chicago, Ill.), and Masako K. Wittner. Observations on the group C streptococcal bacteriophage and lytic enzyme system. J. Bacteriol. 89:496–502. 1965.—The phage-associated lytic enzyme of group C streptococci was assayed by measuring the solubilized portion of radioactive cell walls. By this sen
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8. Subcellular distribution of the soluble lytic transglycosylase in Escherichia coli.
The localization of the major autolytic enzyme, the soluble lytic transglycosylase, in the different cell compartments of Escherichia coli was investigated by immunoelectron microscopy. Ultrathin sections were labeled with a specific antiserum against purified soluble lytic transglycosylase, and the antibody-enzyme complexes were visualized with colloidal pr
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9. Characterization of a Bacteriophage-Induced, Host-Specific Lytic Enzyme from Lysates of Bacillus stearothermophilus Infected with Bacteriophage TP-8
Phage TP-8 lysates of Bacillus stearothermophilus 4S or 4S(8) contain lytic activity exhibiting two pH optima, one at pH 6.5 and the other at pH 7.5. Using a variety of fractionation procedures, the two lytic activities could not be separated. At pH 7.5 the lytic enzyme is an endopeptidase which hydrolyzes the l-alanyl-d-glutamyl linkage in the peptide subun
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10. Production and Ecological Significance of Yeast Cell Wall-Degrading Enzymes from Oerskovia †
Motile actinomycetes capable of degrading walls of viable yeast cells were isolated from soil and identified as Oerskovia xanthineolytica. A lytic assay based on susceptibility of enzyme-treated cells to osmotic shock was developed, and 10 of 15 strains of O. xanthineolytica, Oerskovia turbata, and nonmotile Oerskovia- like organisms from other collections w
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11. Relative Rates of Lysis of Staphylococcal Cell Walls by Lytic Enzymes from Various Bacteriophage Types
Lytic enzymes were isolated from 14 strains of phage-infected Staphylococcus aureus. Cell walls were prepared from the same uninfected strains of bacteria. Comparison of the lytic rates was made for each enzyme, with each of the cell walls as substrate. Differences in the rate of substrate utilization of the various cell wall types exceeded 10-fold. Cell wal
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12. Molecular cloning and nucleotide sequence of the beta-lytic protease gene from Achromobacter lyticus.
Two bacteriolytic enzymes secreted by Achromobacter lyticus M497-1 were purified and identified as being very similar (considering their amino acid composition and N-terminal sequence) to alpha- and beta-lytic proteases from Lysobacter enzymogenes. A 1.8-kb EcoRI fragment containing the structural gene for beta-lytic protease was cloned from A. lyticus chrom