Atividade de plasmina simile e sequencia parcial de enzima fibrino(geno)litica do veneno de Bothrops lanceolatus (Fer de lance)

AUTOR(ES)
DATA DE PUBLICAÇÃO

2005

RESUMO

The main symptoms following bothropic envenoming are necroses, hemorrhage and coagulopathies, originating a characteristic and complex phisiopathology. The work of Lôbo de Araújo et al. (1998) described a single polipeptide chain protein (PII-1A) purified from Bothrops lanceolatus venom, a snake inhabitant of the Martinica island. The immunodiffusion and immunoeletrophoresis analysis showed a single immunoprecipitin line. This protein was characterized as a fibrino(geno)lytic enzyme since was able to hydrolyze the a and b chains of fibrinogen. Using synthetic substrates, the authors demonstrated a strong sterolytic activity against p-TAME. In the present work we continue to study this protein (PII-1A) investigating its activity to activate plasminogen or to present a direct fibrinolytic activity using S-2251ä substrate. The determination of its partial sequence, including N-terminal, was carried through by mass spectrometry - Q-tof that showed molecular weight 28.369 kDa

ASSUNTO(S)

bothrops snake bites hemorragia fibrinolíticos fibrinolytic agents plasmina plasmin mordeduras de cobra envenenamento

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