Integrase C31
Mostrando 1-12 de 15 artigos, teses e dissertações.
-
1. Engenharia genética de um sistema de expressão duradoura e seletiva do VEGF para condição isquêmica baseado no sistema integrase C31 e HRE / Genetic engineering of selective and long term VEGF expression to ischemic condition based on integrase C31 system and HRE
A doença arterial periférica é caracterizada pelo fluxo insuficiente de sangue para as extremidades e afeta mais de 10 milhões de pessoas no mundo podendo ocorrer fortes dores, úlceras não-cicatrizantes e perda do membro afetado. Com o intuito de auxiliar esses pacientes, a terapia gênica vem apresentando novos estudos para estimular o processo de neo
Publicado em: 2010
-
2. Enhanced efficiency through nuclear localization signal fusion on phage φC31-integrase: activity comparison with Cre and FLPe recombinase in mammalian cells
The integrase of the phage ΦC31 recombines an attP site in the phage genome with a chromosomal attB site of its Streptomyces host. We have utilized the integrase-mediated reaction to achieve episomal and genomic deletion of a reporter gene in mammalian cells, and provide the first comparison of its efficiency with other recombinases in a new assay system. T
Oxford University Press.
-
3. Synapsis and DNA cleavage in φC31 integrase-mediated site-specific recombination
The Streptomyces phage φC31 encodes an integrase belonging to the serine recombinase family of site-specific recombinases. The well studied serine recombinases, the resolvase/invertases, bring two recombination sites together in a synapse, and then catalyse a concerted four-strand staggered break in the DNA substrates whilst forming transient covalent attac
Oxford University Press.
-
4. Site-Specific Genomic Integration in Mammalian Cells Mediated by Phage φC31 Integrase
We previously established that the phage φC31 integrase, a site-specific recombinase, mediates efficient integration in the human cell environment at attB and attP phage attachment sites on extrachromosomal vectors. We show here that phage attP sites inserted at various locations in human and mouse chromosomes serve as efficient targets for precise site-spe
American Society for Microbiology.
-
5. A phage integrase directs efficient site-specific integration in human cells
The integrase from the Streptomyces phage φC31 carries out efficient recombination between the attP site in the phage genome and the attB site in the host bacterial chromosome. In this paper, we show that the enzyme also functions in human cells. A plasmid assay system was constructed that measured intramolecular integration of attP into attB. This assay wa
The National Academy of Sciences.
-
6. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family
The genome of the broad host range Streptomyces temperate phage, φC31, is known to integrate into the host chromosome via an enzyme that is a member of the resolvase/invertase family of site-specific recombinases. The recombination properties of this novel integrase on the phage and Streptomyces ambofaciens attachment sites, attP and attB, respectively, wer
The National Academy of Sciences.
-
7. Phage integrases for the construction and manipulation of transgenic mammals
Phage integrases catalyze site-specific, unidirectional recombination between two short att recognition sites. Recombination results in integration when the att sites are present on two different DNA molecules and deletion or inversion when the att sites are on the same molecule. Here we demonstrate the ability of the φC31 integrase to integrate DNA into en
BioMed Central.
-
8. Integration Site for Streptomyces Phage φBT1 and Development of Site-Specific Integrating Vectors
Despite extensive similarities between the genomes of the Streptomyces temperate phages φC31 and φBT1, the attP-int loci are poorly conserved. Here we demonstrate that φBT1 integrates into a different attachment site than φC31. φBT1 attB lies within SCO4848 encoding a 79-amino-acid putative integral membrane protein. Integration vectors based on φBT1 i
American Society for Microbiology.
-
9. The Streptomyces Genome Contains Multiple Pseudo-attB Sites for the φC31-Encoded Site-Specific Recombination System
The integrase from the Streptomyces phage φC31 is a member of the serine recombinase family of site-specific recombinases and is fundamentally different from that of λ or its relatives. Moreover, φC31 int/attP is used widely as an essential component of integration vectors (such as pSET152) employed in the genetic analysis of Streptomyces species. φC31 o
American Society for Microbiology.
-
10. Directed evolution of a recombinase for improved genomic integration at a native human sequence
We previously established that a unidirectional site-specific recombinase, the phage φC31 integrase, can mediate integration into mammalian chromosomes. The enzyme directs integration of plasmids bearing the phage attB recognition site into pseudo attP sites, a set of native sequences related to the phage attP recognition site. Here we use two cycles of DNA
Oxford University Press.
-
11. Two distinct subforms of the retrotransposable DRE element in NC4 strains of Dictyostelium discoideum.
Approximately 2% of the Dictyostelium discoideum genome consists of multiple copies of a retrotransposable element termed DRE (Dictyostelium Repetitive Element). These elements have always been found integrated in a position and orientation-specific manner 50 +/- 4 nucleotides upstream of the coding region of tRNA genes (tDNAs). An intact DRE is 5.7 kb long.
-
12. Lactococcus lactis Lytic Bacteriophages of the P335 Group Are Inhibited by Overexpression of a Truncated CI Repressor
Phages of the P335 group have recently emerged as important taxa among lactococcal phages that disrupt dairy fermentations. DNA sequencing has revealed extensive homologies between the lytic and temperate phages of this group. The P335 lytic phage φ31 encodes a genetic switch region of cI and cro homologs but lacks the phage attachment site and integrase ne
American Society for Microbiology.