Indirect Fluorescence Antibody Test
Mostrando 1-12 de 40 artigos, teses e dissertações.
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1. Where, when, and how the diagnosis of human visceral leishmaniasis is defined: answers from the Brazilian control program
BACKGROUND Timely diagnosis is recommended by the Brazilian Visceral Leishmaniasis (VL) Surveillance and Control Program to reduce case fatality. Attempts at assessing this topic in Brazil are scarce. OBJECTIVE This study aimed to describe where, when, and how the diagnosis of VL has been performed in a Brazilian endemic setting. METHODS Data of all au
Mem. Inst. Oswaldo Cruz. Publicado em: 28/10/2019
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2. The performance of serological tests for Leishmania infantum infection screening in dogs depends on the prevalence of the disease
ABSTRACT Dogs are considered the main reservoir of Leishmania infantum. This protozoan causes visceral leishmaniasis (VL), an uncontrolled urban zoonosis in Brazil. Serological tests and polymerase chain reaction (PCR) on peripheral blood were performed to identify infected dogs in scenarios of higher and lower prevalence of the disease (Teresina and Vitóri
Rev. Inst. Med. trop. S. Paulo. Publicado em: 01/06/2017
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3. ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and the bioassay. Serum samples from 61 contacting dogs were also
Rev. Inst. Med. trop. S. Paulo. Publicado em: 08/04/2016
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4. Toxoplasma Gondii and Neospora Caninum Antibodies in Backyard Chickens in Rio Grande do Sul, Brazil
Toxoplasma gondii and Neospora caninum are two intracellular apicomplexan protozoa with worldwide distribution, and are responsible for reproductive disorders in sheep and cattle. These protozoa may infect a wide variety of domestic and wild animals, including birds, and backyard chickens can be used as sentinels of their infection. Parasites investigation i
Rev. Bras. Cienc. Avic.. Publicado em: 2015-06
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5. A new survey of the serology of human Trypanosoma cruziinfection in the Rio Negro microregion, Brazilian Amazon: a critical analysis
The serology of human Trypanosoma cruziinfection in the Rio Negro microregion is very complex because of the large numbers of false-positive cases that result from low antibody titres and cross-reactions with other infections. In the present study, we collected 4,880 blood samples on filter paper; of these, indirect immunofluorescence (IIF) was strongly reac
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-11
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6. HOSPEDEIROS NATURAIS DA DOENÇA DE CHAGAS EM POPULAÇÕES DE MAMÍFEROS SILVESTRES NA ÁREA DE INFLUÊNCIA DA USINA HIDRELÉTRICA CORUMBÁ IV (GOIÁS): UMA AVALIAÇÃO PRELIMINAR
A preliminary study of the wild fauna host of T. cruzi was carried out within the area of influence of the reservoir of the Corumbá IV hydroelectric power plant where through the faunal rescue of vertebrates specimens of possums, armadillos, rodents, monkeys, foxes and anteaters where collected. Blood samples where collected from a total of 23 wild mammals
Publicado em: 2006
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7. Infecção por Toxoplasma gondii e Neospora caninum em cães e lobos-guará: soroepidemiologia e imunodiagnóstico / Infecção por Toxoplasma gondii e Neospora caninum em cães e lobos-guará: soroepidemiologia e imunodiagnóstico
Toxoplasma gondii and Neospora caninum are two closely related apicomplexan parasites, even though phylogenetic, ultrastructural, antigenic and biological differences have been already described. Seroepidemiological studies have demonstrated that T. gondii infection is often in domestic and sylvatic canids, whereas N. caninum infection is less common, but it
Publicado em: 2006
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8. Produção de antigenos de Neospora caninum e realização de imunofluorescencia indireta em cães
Neospora caninum é um protozoário intracelular obrigatório, que pode infectar canideos domésticos e selvagens, ruminantes e eqüinos, tendo sido descrito em 1988 por DUBEY et al. causando alterações neuromusculares e morte em cães. Para determinar a prevalencia deste parasita na população de cães da cidade de Campo Grande-MS, foram coletadas 245 am
Publicado em: 2004
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9. Evaluation of a quantitative fluorescence immunoassay (FIAX) for detection of serum antibody to Borrelia burgdorferi.
A quantitative, indirect, fluorescence immunoassay (FIAX; Whittaker Bioproducts, Inc.) was compared with the conventional indirect fluorescent-antibody test for detection of serum antibody to Borrelia burgdorferi. FIAX correlated well with the indirect fluorescent-antibody test (r = 0.72). FIAX is a convenient and dependable means of measuring serum antibody
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10. Automation of the Indirect Fluorescent-Antibody Test for Toxoplasmosis
The feasibility of automating the decision-making tasks of the medical technician in the determination of the results of the indirect fluorescent-antibody test for toxoplasmosis was investigated. Two approaches were studied: (i) macroscopic measurement of fluorescence from a large number of organisms (full-field illumination measurements), and (ii) microscop
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11. Indirect fluorescence test for the detection of anti-gonoccocal antibodies.
(1) An indirect fluorescence test for the detection of anti-gonococcal antibody is described. (2) Positive results at a serum dilution of 1 in 16 or above were obtained with sera from 20 per cent. of males and 61 per cent. of females with bacteriologically proven gonorrhoea. 3-8 per cent. of presumed false positive results were given by sera from patients pr
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12. Use of isolated nuclei in the indirect fluorescent-antibody test for human cytomegalovirus infection: comparison with microneutralization, anticomplement, and conventional indirect fluorescent-antibody assays.
Use of an antigen consisting of purified isolated nuclei from a mixture of human cytomegalovirus-infected and uninfected fibroblasts in a 2:1 ratio is a simple and reliable method for eliminating nonspecific fluorescence associated with the presence of Fc-immunoglobulin G receptors in the cytoplasm of infected cells. The specificity obtained with this antige