ISOLATION AND GENOTYPING OF Toxoplasma gondii IN SERONEGATIVE URBAN RATS AND PRESENCE OF ANTIBODIES IN COMMUNICATING DOGS IN BRAZIL
AUTOR(ES)
RUFFOLO, Bruno Bergamo, TOLEDO, Roberta dos Santos, MARTINS, Felippe Danyel Cardoso, BUGNI, Felipe Monteiro, COSTA, Letícia da, MARANA, Elizabete Regina Marangoni, NAVARRO, Italmar Teodorico, GARCIA, João Luis, SU, Chunlei, FREIRE, Roberta Lemos
FONTE
Rev. Inst. Med. trop. S. Paulo
DATA DE PUBLICAÇÃO
08/04/2016
RESUMO
The role of rodents in the epidemiology of toxoplasmosis was investigated in Londrina, Paraná State, Brazil. One hundred and eighty-one Rattus rattus and one Mus musculus were caught in 37 places. Blood and tissues were collected and submitted to the indirect fluorescence antibody test (IFAT) and the bioassay. Serum samples from 61 contacting dogs were also collected. Sixteen rats (8.8%) were positive for Toxoplasma gondii, but just two of them were positive by serology and bioassay test. Antibodies were found in nine (4.9%) rats. Tissues of nine rats bioassayed were positive and four isolates were obtained. Restriction fragment length polymorphism (RFLP) analysis was performed using 12 markers (SAG1, SAG2, SAG2-alt, C22-8, C29-2, L358, PK1, BTUB, GRA6, SAG3, Apico, CS3). Genotyping revealed that the four strains isolated from this study have been isolated before in cats and chickens from Brazil. None of the isolates was identified like clonal archetypal T-types I, II, and III. The rats presented lower serologic Toxoplasma gondii prevalence (8.8%) compared to contacting dogs (70.5%).
Documentos Relacionados
- Risk factors and presence of antibodies to Toxoplasma gondii in dogs from the coast of São Paulo State, Brazil
- Prevalence of antibodies against Toxoplasma gondii and Neospora caninum in dogs
- Seroprevalence of Neospora caninum and Toxoplasma gondii in dogs from an urban area of North-eastern Brazil: a spatial approach
- Serological profile of anti-Toxoplasma gondii antibodies in apparently healthy dogs of the city of Botucatu, São Paulo State, Brazil
- Detection of antibodies to Toxoplasma gondii in wild animals in Brazil