Immunoelectron Microscopy
Mostrando 1-12 de 767 artigos, teses e dissertações.
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1. Identification of the alpha-enolase P46 in the extracellular membrane vesicles of Bacteroides fragilis
BACKGROUND Members of the Bacteroides fragilis group are the most important components of the normal human gut microbiome, but are also major opportunistic pathogens that are responsible for significant mortality, especially in the case of bacteraemia and other severe infections, such as intra-abdominal abscesses. Up to now, several virulence factors have b
Mem. Inst. Oswaldo Cruz. Publicado em: 2018-03
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2. Do Archaea and bacteria co-infection have a role in the pathogenesis of chronic chagasic cardiopathy?
Chronic cardiopathy (CC) in Chagas disease is a fibrotic myocarditis with C5b-9 complement deposition. Mycoplasma and Chlamydia may interfere with the complement response. Proteolytic enzymes and archaeal genes that have been described in Trypanosoma cruzi may increase its virulence. Here we tested the hypothesis that different ratios of Mycoplasma, Chlamydi
Memórias do Instituto Oswaldo Cruz. Publicado em: 2009-07
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3. Production and characterization of monoclonal antibodies against the identical region of LigA and LigB proteins from Leptospira interrogans / Produção e caracterização de anticorpos monoclonais contra a região idêntica das proteínas LigA e LigB de Leptospira interrogans
Leptospirosis is a zoonotic disease caused by pathogenic bacteria belonging to the Leptospira genus. Several mammals may carry the agent, and rats are the most important source of human infection in urban settings. The wide spectrum of clinical manifestations varies from mild cases, with fever and headaches, to severe presentations, with liver and kidney fai
Publicado em: 2009
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4. A resposta inflamatória na urticária aguda associada a medicamentos: avaliação imunoistoquímica e imunoeletrônica da unidade microvascular da derme / The inflammatory response in acute drug-induced urticaria: immunohistochemistry and ultrastructure study of dermal microsvascular unit
INTRODUÇÃO: O conhecimento sobre os tipos celulares envolvidos na patogenia da urticária constitui um elemento essencial para a compreensão da fisiopatologia desta doença. Poucos autores têm dado atenção às interações entre mastócitos e dendrócitos da derme na urticária. Os objetivos deste estudo são orientados no sentido de descreverem-se os
Publicado em: 2007
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5. Imunolocalização das Heat Shock Proteins (HSPs) 60 e 70 na placenta bovina / Immunolocalization of Heat Shock Proteins (HSPs) 60 e 70 in bovine placenta
Heat Shock Proteins (HSP) can be found in any kind of cell. These proteins are classified according to their molecular weight and their known families include the HSP 27, 60, 70, 90 and 110 kDa. Among these, HSP 60 and 70 are the ones of interest in reproduction. They were known as chaperonines because of their capacity to fold and unfold other proteins into
Publicado em: 2005
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6. Antigen incorporation on Cryptosporidium parvum oocyst walls
Cryptosporidium parvum oocysts are the infective stages responsible for transmission and survival of the organism in the environment. In the present work we show that the oocyst wall, far from being a static structure, is able to incorporate antigens by a mechanism involving vesicle fusion with the wall, and the incorporation of the antigen to the outer oocy
Memórias do Instituto Oswaldo Cruz. Publicado em: 2001-02
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7. Use of immunoelectron microscopy to show Ebola virus during the 1989 United States epizootic.
A filovirus, serologically related to Ebola virus, was detected by "post-embedment" immunoelectron microscopical examination of MA-104 cells. These had been infected by inoculation with serum samples obtained during the 1989 epizootic in cynomolgus monkeys (Macaca fascicularis), imported from the Philippines and maintained at Reston, Virginia, USA, a primate
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8. Rapid adenovirus typing by immunoelectron microscopy
A rapid method of typing adenoviruses by immunoelectron microscopy is discribed. This emphasizes the value of an electron microscope in diagnostic virology, especially when a rapid result is required in epidemiology.
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9. Use of immunoelectron microscopy to demonstrate Francisella tularensis.
Three immunoelectron microscopy (IEM) methods were employed to show laboratory-cultivated Francisella tularensis. By the IEM assays, F. tularensis was distinguished from four antigenically distinct gram-negative bacteria. IEM should be a valuable tool for confirming presumptive isolates of F. tularensis and may potentially be useful for demonstrating other m
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10. Rapid identification of Ebola virus and related filoviruses in fluid specimens using indirect immunoelectron microscopy.
Recent filoviral outbreaks in animal primates have raised public awareness of the potential for filoviruses to become a public health concern; methods that efficiently identify these viruses are therefore of high priority. An indirect immunoelectron microscopy method, which uses homologous guinea pig polyclonal antiserum, successfully identified Ebola-relate
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11. Use of computerized multidimensional scaling to compare immunoelectron microscopy data with protein near-neighbor information: application to the 30S ribosome from Escherichia coli.
A three-dimensional model of the protein arrangement in the Escherichia coli 30S ribosome was constructed by using computerized multidimensional scaling of immunoelectron microscope data. This enabled data comparison between the new electron microscope technique and other methods such as crosslinking, chemical protection, affinity labeling, energy transfer,
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12. Ultrastructural localization of dipicolinic acid in dormant spores of Bacillus subtilis by immunoelectron microscopy with colloidal gold particles.
The localization of dipicolinic acid in dormant spores of Bacillus subtilis was examined by an immunoelectron microscopy method with colloidal gold-immunoglobulin G complex. The colloidal gold particles were distributed mainly in the core regions of dormant spores and were not observed in those of germinated or autoclaved spores. This result clearly demonstr