Immunoelectron Microscopy
Mostrando 13-24 de 767 artigos, teses e dissertações.
-
13. Immunoelectron microscopy of lipopolysaccharide in Chlamydia trachomatis.
Monoclonal antibodies (MAb) specific for Chlamydia trachomatis lipopolysaccharide (LPS) and major outer membrane protein (MOMP) were used for immunoelectron microscopy analysis. MAb specific for MOMP showed strong reaction with the chlamydial surface, whereas MAb specific for LPS showed strong association of gold particles with the periphery of the chlamydia
-
14. Ultrastructural changes in avian Chlamydia psittaci serovar A-, B-, and D-infected Buffalo Green Monkey cells.
In order to find an explanation for the observed differences in levels of pathogenicity in turkeys of Chlamydia psittaci 84/55 (avian serovar A), 89/1326 (avian serovar B), 92/1293 (avian serovar D), and the Texas Turkey strain (avian serovar D) (P.B. Wyrick, J. Choong, S.T. Knight, D. Goyeau, E.S. Stuart, and A.B. MacDonald, Immunol. Infect. Dis. 4:131-141,
-
15. Locating transcribed and non-transcribed rDNA spacer sequences within the nucleolus by in situ hybridization and immunoelectron microscopy.
Immunoelectron microscopy and in situ hybridization have been used to investigate the precise location of transcribed and non-transcribed rDNA spacer sequences. Whereas a 5'-external transcribed spacer sequence is predominantly visualized in the fibrillar centers of nucleoli, a non-transcribed spacer sequence is preferentially detected in the interstices, in
-
16. Localization of fibronectin, laminin-entactin, and entactin in Reichert's membrane by immunoelectron microscopy.
Immunoelectron microscopy using protein A-colloidal gold complexes of different sizes was used to study the relative distribution of extracellular matrix glycoproteins within Reichert's membrane (RM) of 13.5-day mouse embryos. Labelling for fibronectin was distributed asymmetrically; the highest concentration occurring in the outermost layer adjacent to the
-
17. Characterization of the Saccharomyces Golgi complex through the cell cycle by immunoelectron microscopy.
The membrane compartments responsible for Golgi functions in wild-type Saccharomyces cerevisiae were identified and characterized by immunoelectron microscopy. Using improved fixation methods, Golgi compartments were identified by labeling with antibodies specific for alpha 1-6 mannose linkages, the Sec7 protein, or the Ypt1 protein. The compartments labeled
-
18. Advantages of fixed stored materials for immunoelectron microscopy, with special reference to the study of malignant lymphomas.
-
19. Immunoelectron microscopic localization of ubiquitin in hepatoma cells.
Ubiquitin, a 76 amino acid protein, is covalently attached to abnormal and short-lived proteins, thus marking them for ATP-dependent proteolysis in eukaryotic cells. Free (unconjugated) ubiquitin was localized in hepatoma cells using affinity purified anti-ubiquitin antibodies and colloidal gold immunoelectron microscopy. The anti-ubiquitin antibodies recogn
-
20. Localization of the tube precipitin and complement fixation antigens of Coccidioides immitis by immunoelectron microscopy with murine monoclonal antibodies.
The cellular localization of the tube precipitin (TP) and complement fixation (CF) antigens of Coccidioides immitis was examined by immunoelectron microscopy with murine immunoglobulin G1 monoclonal antibodies directed against the TP and CF antigens, respectively. Immunoelectron microscopic analyses of saprobic- and parasitic-phase cells showed that the TP a
-
21. Localization of smooth muscle-like contractile proteins in kidney by immunoelectron microscopy.
Contractile proteins of smooth muscle type were found in kidney cells by immunological methods. The reactions of rabbit anti-actin, anti-heavy meromyosin and anti-myosin antisera with rat kidney were investigated by immunoelectron microscopy. Anti-actin stained specifically the foot processes of epithelial cells in the glomerulus, the basal processes of tubu
-
22. Demonstration of a Cell-Surface Antigen Associated with Murine Sarcoma Virus by Immunoelectron Microscopy
Cells transformed by murine sarcoma virus have been examined for the presence of a new virus-associated cell-surface antigen by immunoelectron microscopy. A common antigen has been detected on the surface of nonproductively transformed cells that were induced by two different strains of murine sarcoma virus, Kirsten and Moloney. This antigen shows crossreact
-
23. Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores.
Ultrastructural localization of GerAB, one of the proteins of Bacillus subtilis spores related to L-alanine-initiated germination, was investigated by immunoelectron microscopy with antipeptide (residues 61 to 80 of GerAB) antiserum and a colloidal gold-immunoglobulin G complex. Immunogold particles were visualized in the boundary region between the cortex a
-
24. Immunoelectron microscopic localization of acidic intracellular compartments in hepatoma cells.
Using protein A-colloidal gold immunoelectron microscopy and monospecific antibodies to the weak base primaquine, we have delineated acidic intracellular compartments in the human hepatoma cell line, HepG2. Primaquine specifically accumulated within endocytotic compartments (including CURL vesicles, multivesicular bodies and lysosomes). In addition, the Golg