Human Salivary Amylase
Mostrando 1-12 de 21 artigos, teses e dissertações.
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1. Enamel and dentine demineralization by a combination of starch and sucrose in a biofilm – caries model
Abstract Sucrose is the most cariogenic dietary carbohydrate and starch is considered non-cariogenic for enamel and moderately cariogenic for dentine. However, the cariogenicity of the combination of starch and sucrose remains unclear. The aim of this study was to evaluate the effect of this combination on Streptococcus mutans biofilm composition and enamel
Braz. oral res.. Publicado em: 20/05/2016
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2. Atividade inibitória de extratos vegetais do cerrado sobre alfa-amilases
As α-amilases, enzimas produzidas por animais, vegetais e microrganismos, participam de etapas extremamente importantes no metabolismo de carboidratos. A sobrevivência dos seres vivos que dependem do aproveitamento de carboidratos como fonte energética está associada à atividade das amilases. A atividade dessas enzimas também está envolvida com a
Publicado em: 2008
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3. Produção de uma fração concentrada em alfa-amilase salivar humana (HSA) como alvo para descoberta de novos inibidores e fracionamento do extrato hidroalcóolico da casca de Pouteria sp
Capítulo II: As alfa-amilases (α-1,4-D-glicano-4-glicanohidrolases, EC 3.2.1.1) são enzimas responsáveis pela digestão de carboidratos e são encontradas na saliva e no suco pancreático. As seqüências primárias das alfa-amilases salivar (HSA) e pancreática (HPA) exibem 99% de similaridade estrutural o que sugere que a HSA pode ser um alvo de es
Publicado em: 2008
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4. Differential expression of salivary (Amy1) and pancreatic (Amy2) human amylase loci in prenatal and postnatal development.
The age-dependent development of alpha-amylase expression in utero and during the first two years of life is reported. Separation of salivary and pancreatic amylase isozymes in a discontinuous buffered sheet polyacrylamide electrophoretic system, with subsequent densitometry, provides a reliable semiquantitative method of estimating the proportions of saliva
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5. A 78-kilobase region of mouse chromosome 3 contains salivary and pancreatic amylase genes and a pseudogene.
Genetic studies have demonstrated that salivary and pancreatic amylase genes are closely linked in human and mouse. To analyze the arrangement of genes within the amylase cluster, a library of YBR mouse genomic DNA was cloned in the cosmid vector pJB8. Clones containing amylase genes were identified by hybridization with amylase cDNA probes. Salivary and pan
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6. Role of Streptococcus gordonii Amylase-Binding Protein A in Adhesion to Hydroxyapatite, Starch Metabolism, and Biofilm Formation
Interactions between bacteria and salivary components are thought to be important in the establishment and ecology of the oral microflora. α-Amylase, the predominant salivary enzyme in humans, binds to Streptococcus gordonii, a primary colonizer of the tooth. Previous studies have implicated this interaction in adhesion of the bacteria to salivary pellicles
American Society for Microbiology.
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7. Characterization of salivary alpha-amylase binding to Streptococcus sanguis.
The purpose of this study was to identify the major salivary components which interact with oral bacteria and to determine the mechanism(s) responsible for their binding to the bacterial surface. Strains of Streptococcus sanguis, Streptococcus mitis, Streptococcus mutans, and Actinomyces viscosus were incubated for 2 h in freshly collected human submandibula
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8. Concerted evolution of human amylase genes.
Cosmid clones containing 250 kilobases of genomic DNA from the human amylase gene cluster have been isolated. These clones contain seven distinct amylase genes which appear to comprise the complete multigene family. By sequence comparison with the cDNAs, we have identified two pancreatic amylase genes and three salivary amylase genes. Two truncated pseudogen
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9. Expression of the human amylase genes: recent origin of a salivary amylase promoter from an actin pseudogene.
The human genes encoding salivary amylase (AMY1) and pancreatic amylase (AMY2) are nearly identical in structure and sequence. We have used ribonuclease protection studies to identify the functional gene copies in this multigene family. Riboprobes derived from each gene were hybridized to RNA from human pancreas, parotid and liver. The sizes of the protected
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10. Effects of alpha-amylase on in vitro growth of Legionella pneumophila.
Sterile parotid saliva inhibited growth of Legionella pneumophila on solid media, and the salivary component involved in this inhibition has been shown to be amylase. Disk diffusion and well plate assays were used to study possible mechanisms for this effect. The amylolytic activity of saliva copurified with inhibitory activity, and both activities were sens
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11. Alpha-amylase gene transcription in tissues of normal dog.
We studied the distribution of alpha-amylase mRNA in normal dog tissues by northern blotting (NB) and reverse transcription-polymerase chain reaction (RT-PCR) with human pancreatic (AMY2) and salivary (AMY1) alpha-amylase cDNA-specific primers. Analysis of poly(A+) RNA from various normal tissues by NB indicated the presence of detectable levels of alpha-amy
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12. Interactions of Streptococcus mutans Fimbria-Associated Surface Proteins with Salivary Components
Streptococcus mutans has been implicated as the major causative agent of human dental caries. S. mutans binds to saliva-coated tooth surfaces, and previous studies suggested that fimbriae may play a role in the initial bacterial adherence to salivary components. The objectives of this study were to establish the ability of an S. mutans fimbria preparati
American Society for Microbiology.