Grp78
Mostrando 13-24 de 113 artigos, teses e dissertações.
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13. Inibição do proteasoma aumenta o estresse oxidativo e bloqueia a resposta da NADPH oxidase a estímulos em células musculares lisas vasculares / Proteasome Inhibiton increases oxidative stress and disrupts NADPH oxidase response to stimuli in vascular smooth muscle cells
Cellular processes governing vascular Nox family NADPH oxidases in disease conditions are unclear. Since redox processes are intrinsic to cell stress response, we asked whether oxidative stress merges with other types of stress via Nox(es). We focused on proteasome inhibition as a relevant stress condition. Vascular smooth muscle cells (VSMC) incubation with
Publicado em: 2009
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14. Avaliação da hiperhomocisteinemia leve na evolução da aterosclerose em camundongos normais, com hemofilia B, deficiencia dos receptores para LDL ou da apolipoproteina E / Evaluation of mild hyperhomocysteinemia in the development of atherosclerosis in normal, hemophiliac B, LDL receptor-deficient or apolipoprotein E-deficient mice
This study evaluated the effect of controlled mild hyperhomocysteinemia (HHcy) in the development of atherosclerosis using normal, apolipoprotein E-deficient (apoE-/-), LDL receptor-deficient (LDLR-/-), or hemophilia B mice. We quantified atherosclerotic lesions at the aortic sinus and analyzed endoplasmic reticulum (RE) stress by western-blot technique. Con
Publicado em: 2008
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15. Estresse nitro-oxidativo induzido por soro urêmico in vivo e in vitro
Pacientes de doença renal crônica (DRC) apresentam maiores riscos de desenvolver doença cardiovascular (DCV) que pacientes com função renal normal. Dentre vários fatores quem contribuem para patologia vascular, inflamação, calcificação e estresse oxidativo têm sido considerados fatores de risco não tradicionais. A uremia, peculiar somente à popu
Publicado em: 2007
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16. Chlorhexidine toxicity injected in the paw of mice and added to cultured L929 fibroblasts / Toxicidade da clorexidina injetada na pata de camundongos e adicionada em cultura de fibroblastos L929
Como a clorexidina (CHX) tem sido recomendada como solução irrigadora de canais radiculares e como curativo de demora, o objetivo do presente estudo foi caracterizar in vivo a lesão induzida pela injeção de CHX a 0,125, 0,25, 0,5 e 1,0% na pata de camundongos em intervalos de tempo selecionados (24 e 48 horas e 7 e 14 dias) e in vitro o modo e a causa m
Publicado em: 2007
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17. Uso de técnicas de proteoma e genoma funcional para revelar as bases moleculares da ação anti-tumoral de ácido retinóico / Use of proteomics and functional genomics to unravel the molecular mechanisms of retinoic acid as an anti-tumor agent
Controlar a proliferação celular de tumores é um objetivo que vem sendo perseguido há décadas, com moderado sucesso na maioria dos casos. Dentre os diversos tipos de tumores que atingem a humanidade, alguns gliomas são considerados os mais fatais, por haver pouca ou nenhuma alternativa de tratamento efetivo. Agentes que apresentam propriedades anti-tum
Publicado em: 2005
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18. Família gênica BiP em plantas: expressão diferencial, indução de estresse e papel de proteção contra estresses fisiológicos
Enquanto, em mamíferos e leveduras, a proteína Binding Protein (BiP) é representada por um único gene, em diversas plantas, tais como milho, Arabidopsis, tabaco e soja, a BiP é codificada por uma família multigênica. A análise e a caracterização sistemáticas da expressão de BiP de soja têm revelado a existência de múltiplos mecanismos complexo
Brazilian Journal of Plant Physiology. Publicado em: 2003-08
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19. Estudo da proteína de choque térmico GRP78 para o desenvolvimento de um sistema de receptor-ligante para o câncer de próstata / Use of the heat-shock protein GRP78 for the development of a receptor-ligand system in prostate cancer
Introduction: Despite the advances in diagnosis and treatment, advanced prostate cancer remains a lethal condition. Improved methods of therapy are needed to reduce the morbidity and mortality rates associated with this disease. The Glucose-regulated protein-78 (GRP78), a stress-responsive heat-shock protein involved in antigen presentation, was recently des
Publicado em: 2003
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20. Overexpression of GRP78 mitigates stress induction of glucose regulated proteins and blocks secretion of selective proteins in Chinese hamster ovary cells.
GRP78 is a resident protein of the endoplasmic reticulum (ER) and a member of the glucose regulated protein (GRP) family. Many secretion incompetent proteins are found in stable association with GRP78 and are retained in the ER. Some proteins which are destined for secretion transiently associate with GRP78. To further increase our understanding of the role
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21. Dissociation of glucose-regulated protein Grp78 and Grp78-IgE Fc complexes by ATP.
Recent studies have shown that ATP can dissociate dimers of the glucose-regulated protein Grp78 to monomers. In the present study, we have used purified recombinant Grp78 from Escherichia coli to investigate this reaction in more detail. During the course of the Grp78 dimer-monomer conversion, a stable Grp78 monomer-ATP complex is formed. Upon removal of the
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22. Enhanced transcription of the 78,000-dalton glucose-regulated protein (GRP78) gene and association of GRP78 with immunoglobulin light chains in a nonsecreting B-cell myeloma line (NS-1).
The 78,000-dalton glucose-regulated protein (GRP78) is a stress-inducible protein localized in the endoplasmic reticulum. It has been identified as the immunoglobulin heavy-chain-binding protein. We report here a high level of GRP78 expression in a B-cell myeloma line, NS-1, which produces only kappa light-chain proteins but is unable to secrete them. GRP78
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23. Reduction of endogenous GRP78 levels improves secretion of a heterologous protein in CHO cells.
GRP78 is localized in the endoplasmic reticulum and associates with improperly folded or underglycosylated proteins. The role of GRP78 in secretion was studied in Chinese hamster ovary cells expressing a tissue plasminogen activator (tPA) variant which lacks potential N-linked glycosylation site sequences because of mutagenesis. The expression of variant tPA
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24. Interactions of liver Grp78 and Escherichia coli recombinant Grp78 with ATP: multiple species and disaggregation.
The hamster gene encoding the 78-kDa glucose-regulated protein (Grp78) was expressed in Escherichia coli as a fusion protein with glutathione S-transferase. After induction with isopropyl beta-D-thiogalactopyranoside, the recombinant Grp78 was purified to homogeneity by affinity column chromatography of the fusion protein followed by thrombin cleavage. The p