Gfp Green Fluorescent Protein
Mostrando 13-24 de 778 artigos, teses e dissertações.
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13. Retention of a recombinant GFP protein expressed by the yellow fever 17D virus in the E/NS1 intergenic region in the endoplasmic reticulum
The flaviviral envelope proteins, E protein and precursor membrane protein, are mainly associated with the endoplasmic reticulum (ER) through two transmembrane (TM) domains that are exposed to the luminal face of this compartment. Their retention is associated with the viral assembly process. ER-retrieval motifs were mapped at the carboxy terminus of these e
Memórias do Instituto Oswaldo Cruz. Publicado em: 2012-03
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14. Análise funcional de genes expressos em glândula esofágica dorsal de meloidogyne incógnita envolvidos no fitoparasitismo
Os fitonematóides do gênero Meloidogyne estão entre os mais prejudiciais para a economia agrícola mundial e são intensamente estudados devido aos danos causados a uma grande variedade de culturas agronomicamente importantes. Técnicas convencionais de controle como rotação de culturas e uso de nematicidas têm se mostrado ineficazes e danosas, fazendo
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/07/2011
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15. Detecção, transmissão e efeitos de Sclerotinia sclerotiorum em sementes de soja / Detection, transmission and effects of Sclerotinia sclerotiorum in soybean seeds
Entre as doenças de maior destaque na cultura da soja encontra-se o mofo-branco, cujo agente etiológico é o fungo Sclerotinia sclerotiorum, agente causal da podridão-de-esclerotinia, murcha-de-esclerotinia ou mofo- branco, em muitas espécies cultivadas e de plantas daninhas. A associação deste fungo com sementes de espécies tão diversas é epidemiol
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 18/03/2011
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16. Optimizing expression and purification of an ATP-binding gene gsiA from Escherichia coli k-12 by using GFP fusion
The cloning, expression and purification of the glutathione (sulfur) import system ATP-binding protein (gsiA) was carried out. The coding sequence of Escherichia coli gsiA, which encodes the ATP-binding protein of a glutathione importer, was amplified by PCR, and then inserted into a prokaryotic expression vector pWaldo-GFPe harboring green fluorescent prote
Genetics and Molecular Biology. Publicado em: 16/09/2011
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17. Identification and characterization of two critical sequences in SV40PolyA that activate the green fluorescent protein reporter gene
Alu repeats or Line-1-ORF2 (ORF2) inhibit expression of the green fluorescent protein (GFP) gene when inserted downstream of this gene in the vector pEGFP-C1. In this work, we studied cis-acting elements that eliminated the repression of GFP gene expression induced by Alu and ORF2 and sequence characteristics of these elements. We found that sense and antise
Genetics and Molecular Biology. Publicado em: 17/06/2011
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18. Oviduct-specific expression of tissue plasminogen activator in laying hens
Egg-laying hens are important candidate bioreactors for pharmaceutical protein production because of the amenability of their eggs for protein expression. In this study, we constructed an oviduct-specific vector containing tissue plasminogen activator (tPA) protein and green fluorescent protein (pL-2.8OVtPAGFP) and assessed its expression in vitro and in viv
Genetics and Molecular Biology. Publicado em: 08/04/2011
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19. Structural interaction between GFP-labeled diazotrophic endophytic bacterium Herbaspirillum seropedicae RAM10 and pineapple plantlets 'Vitória'
The events involved in the structural interaction between the diazotrophic endophytic bacterium Herbaspirillum seropedicae, strain RAM10, labeled with green fluorescent protein, and pineapple plantlets 'Vitória' were evaluated by means of bright-field and fluorescence microscopy, combined with scanning electron microscopy for 28 days after inoculation. Afte
Brazilian Journal of Microbiology. Publicado em: 2011-03
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20. Characterization of a small cryptic plasmid from endophytic Pantoea agglomerans and its use in the construction of an expression vector
A circular cryptic plasmid named pPAGA (2,734 bp) was isolated from Pantoea agglomerans strain EGE6 (an endophytic bacterial isolate from eucalyptus). Sequence analysis revealed that the plasmid has a G+C content of 51% and contains four potential ORFs, 238(A), 250(B), 131(C), and 129(D) amino acids in length without homology to known proteins. The shuttle v
Genetics and Molecular Biology. Publicado em: 19/11/2010
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21. Transferência de genes heterólogos para células corneanas epiteliais primárias utilizando vetor lentivírus
OBJETIVO: Avaliar a transferência de genes heterólogos expressando a proteína "Green Fluorescent Protein" (GFP) para células corneanas epiteliais primárias ex vivo utilizando vetor lentivírus. MÉTODOS: Tecido corneoescleral de coelhos foi usado para obtenção de suspensão de células corneanas epitelias. As células foram semeadas na densidade de 5�
Arquivos Brasileiros de Oftalmologia. Publicado em: 2010-10
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22. Efeito de candidatos a supressores de silenciamento gênico viral em expressão de proteína recombinate em plantas
The aim of this work was to increase the efficiency of transient heterolog protein expression system using viral gene-silencing suppressors and Agrobacterium integrated. The use of plants as bioreactor is a promising strategy for large scale production of the heterolog protein. However, the use of genetically modified plants may lead to low protein expressio
Publicado em: 2010
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23. Produção de vetores de transformação de plantas contendo fusões das proteínas ASR (Abcisic acid, Stress and Ripening) e GFP (Green Fluorescent Protein) visando a determinação da localização subcelular das proteínas ASR de arroz (Oryza sativa L.)
Os genes ASR (ABA, Stress and Ripening) foram descritos, inicialmente, em tomate (Solanum lycopersicum) e, em seguida, identificados em diversas espécies de plantas. A expressão dos genes ASR é induzida por ácido abscísico (ABA) e seus níveis de transcritos são rapidamente aumentados em resposta à salinidade e à seca. Estudos de localização subcel
Publicado em: 2010
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24. Experimental model of gene transfection in healthy canine myocardium. Perspectives of gene therapy for ischemic heart disease
Objective - To assess the transfection of the gene that encodes green fluorescent protein (GFP) through direct intramyocardial injection. Methods - The pREGFP plasmid vector was used. The EGFP gene was inserted downstream from the constitutive promoter of the Rous sarcoma virus. Five male dogs were used (mean weight 13.5 kg), in which 0.5 mL of saline soluti
Publicado em: 2010