Gene Hexa
Mostrando 1-12 de 23 artigos, teses e dissertações.
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1. Identification and validation of novel EST-SSR markers in olives
ABSTRACT: The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) marke
Sci. agric. (Piracicaba, Braz.). Publicado em: 2017-06
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2. Construção de vetor baculoviral modificado capaz de produzir proteínas fusionadas à poliedrina
Este trabalho objetivou construir um vetor baculoviral modificado capaz de expressar proteínas heterólogas fusionadas à poliedrina e à cauda de hexahistidina e utilizar esse vetor para expressão e purificação da proteína capsidial de vírus do complexo viral do alho. Via PCR, foi sintetizado o fragmento PH (gene da poliedrina fusionado a uma cauda de
Publicado em: 2008
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3. Biochemical and immunological characterization of ATP- diphosphohydrolases 1 and 2 from Schistosoma mansoni parasite / Caracterização bioquímica e imunológica das enzimas recombinantes ATP-difosfohidrolases 1 e 2 do parasita Schistosoma mansoni
ATPDases or ATP-diphosphohydrolases are enzymes that cleave ATP and ADP to AMP and Pi and are involved in inhibition of platelet aggregation. In the parasite Schistosoma mansoni our group had identified and cloned the ATPDase1 gene and localized the protein on the tegument surface. Recently, we cloned the ATPDase2 gene using S. mansoni EST databank informati
Publicado em: 2008
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4. The frequency of Tay-Sachs disease causing mutations in the Brazilian Jewish population justifies a carrier screening program
CONTEXTO: A doença de Tay-Sachs é uma doença autossômica recessiva caracterizada por uma degeneração neurológica progressiva, fatal na primeira infância. Na população judaica Ashkenazita a incidência da doença é de um para cada 3.500 nascimentos, e a freqüência de portadores é de um para cada 29 indivíduos. Programas de triagem de portadores
Sao Paulo Medical Journal. Publicado em: 2001-07
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5. Heteroduplex DNA mismatch repair system of Streptococcus pneumoniae: cloning and expression of the hexA gene.
Mutations affecting heteroduplex DNA mismatch repair in Streptococcus pneumoniae were localized in two genes, hexA and hexB, by fractionation of restriction fragments carrying mutant alleles. A fragment containing the hexA4 allele was cloned in the S. pneumoniae cloning system, and the hexA+ allele was introduced into the recombinant plasmid by chromosomal f
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6. Nucleotide sequence of the hexA gene for DNA mismatch repair in Streptococcus pneumoniae and homology of hexA to mutS of Escherichia coli and Salmonella typhimurium.
The Hex system of heteroduplex DNA base mismatch repair operates in Streptococcus pneumoniae after transformation and replication to correct donor and nascent DNA strands, respectively. A functionally similar system, called Mut, operates in Escherichia coli and Salmonella typhimurium. The nucleotide sequence of a 3.8-kilobase segment from the S. pneumoniae c
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7. Mismatch repair genes of Streptococcus pneumoniae: HexA confers a mutator phenotype in Escherichia coli by negative complementation.
DNA repair systems able to correct base pair mismatches within newly replicated DNA or within heteroduplex molecules produced during recombination are widespread among living organisms. Evidence that such generalized mismatch repair systems evolved from a common ancestor is particularly strong for two of them, the Hex system of the gram-positive Streptococcu
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8. Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae.
The mutS gene product of Escherichia coli and Salmonella typhimurium is one of at least four proteins required for methyl-directed mismatch repair in these organisms. A functionally similar repair system in Streptococcus pneumoniae requires the hex genes. We have sequenced the S. typhimurium mutS gene, showing that it encodes a 96-kilodalton protein. Amino-t
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9. Frequency of Mutation to Rifampin Resistance in Streptococcus pneumoniae Clinical Strains: hexA and hexB Polymorphisms Do Not Account for Hypermutation
The frequency of mutation to rifampin resistance of 200 clinical Streptococcus pneumoniae isolates was examined. Two peaks were observed in the distribution, with mode frequencies of 2.5 × 10−7 (20% of isolates) and 2.5 × 10−8. The hexA and hexB gene entire sequences were analyzed in 13 isolates. Sequences from both hypermutable and “normomutable”
American Society for Microbiology.
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10. Azotobacter vinelandii mutS: nucleotide sequence and mutant analysis.
An Azotobacter vinelandii homolog to the Salmonella typhimurium mutS gene was discovered upstream of the fdxA gene. The product of this gene is much more similar to S. typhimurium MutS than either is to the HexA protein of Streptococcus pneumoniae. An A. vinelandii delta mutS mutant strain was shown to have a spontaneous mutation frequency 65-fold greater th
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11. The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes.
The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which is interrupted by a 68 bp long intron. T
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12. Targeted disruption of the Hexa gene results in mice with biochemical and pathologic features of Tay-Sachs disease.
Tay-Sachs disease, the prototype of the GM2 gangliosidoses, is a catastrophic neurodegenerative disorder of infancy. The disease is caused by mutations in the HEXA gene resulting in an absence of the lysosomal enzyme, beta-hexosaminidase A. As a consequence of the enzyme deficiency, GM2 ganglioside accumulates progressively, beginning early in fetal life, to