Fructosyltransferase
Mostrando 1-12 de 59 artigos, teses e dissertações.
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1. SYNTHESIS AND CHARACTERIZATION OF FRUCTOSYLTRANSFERASE FROM Aspergillus oryzae IPT-301 FOR HIGH FRUCTOOLIGOSACCHARIDES PRODUCTION
Abstract Fructooligosaccharides (FOS) are mainly produced by microbial fructosyltransferases (FTase, E.C.2.4.1.9), and Aspergillus oryzae IPT-301 has shown high fructosyl transferring and low hydrolytic activities, which leads to high FOS production yields, but the main operating parameters for its best performance have been scarcely studied. Thus, this work
Braz. J. Chem. Eng.. Publicado em: 30/09/2019
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2. Estudos sobre as propriedades catalíticas da frutosiltransferase de Rhodotorula sp. livre e imobilizada em suporte inorgânico / Catalytic properties of fructosyltransferase from Rhodotorula sp., free and immobilized on inorganic support
A obtenção de fruto-oligossacarídeos (FOS), um açúcar prebiótico de vasta aplicação industrial, por enzimas imobilizadas abrange dois conceitos em grande expansão: aplicação industrial de enzimas imobilizadas e alimentos funcionais. A partir de flores da Mata Atlântica Brasileira uma cepa foi isolada e identificada como Rhodotorula sp. LEB-V10 e
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 13/02/2012
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3. Media optimization for β-Fructofuranosidase production by Aspergillus oryzae
β-Fructofuranosidase production by Aspergillus oryzae IPT301 was maximized in shake flasks. Response Surface Methodology (RSM) involving Small Central Composite Design was adopted to evaluate the fructosyltransferase (FTase) activity by changing three medium component concentrations: sucrose, urea and yeast extract. The optimal set of conditions for maximum
Brazilian Journal of Chemical Engineering. Publicado em: 2012-03
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4. Thermal stability of the immobilized fructosyltransferase from Rhodotorula sp
The thermal stability of the extracellular fructosyltransferase (FTase) from Rhodotorula sp., recovered from cultivation medium by ethanol precipitation and immobilized onto niobium ore, was studied by Arrhenius plot, half - life profile, half - inactivation temperature (T50) and thermodynamic parameters. The Arrhenius plot showed two different behaviors wit
Brazilian Journal of Chemical Engineering. Publicado em: 2011-09
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5. Modelagem cinética e simulação de processo de produção de frutooligossacarídeos por frutosiltransferase de Rhodotorula sp. livre e imobilizada / : Kinetic modelling and process simulation of fructooligosaccharides production by free and immobilized fructosyltransferase of Rhodotorula sp
Os frutooligossacarídeos são considerados prebióticos, uma vez que promovem seletivamente o crescimento de micro-organismos probióticos como Lactobacillus acidophillus e Bifidobacterium bifidus. Novas enzimas, na forma livre ou imobilizada, representam uma das possibilidades para síntese destes compostos. Neste trabalho procedeu-se ao estudo da modelage
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 10/12/2010
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6. Mutagenesis of Aspergillus oryzae IPT-301 to improve the production of β-fructofuranosidase
Aspergillus oryzae IPT-301, previously reported as a β-fructofuranosidase producing microorganism, was successfully mutated using UV irradiation at 253.7 nm followed by the screening of survivors resistant to certain stress conditions. Strains were first subjected to the β-fructofuranosidase activity assay using a portion from the colony grown in Petri dis
Brazilian Journal of Microbiology. Publicado em: 2010-03
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7. Seleção de microrganismos produtores de frutosiltransferase e estudo das propriedades bioquimicas da frutosiltransferase de Penicillium sp / Screening of microrganisms for transfructosylating activity and study of biochemical properties of fructosyltransferase from Penicillium sp
t: Impulsionados pela grande demanda por alimentos ¿saudáveis¿ e de calorias controladas, têm surgido desde os anos 80 um grande número de adoçantes alternativos e, entre eles, diversos oligossacarídeos. Entre os oligossacarídeos mais estudados estão os frutooligossacarídeos (FOS), que se tornaram mais importantes por suas propriedades funcionais q
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 20/02/2009
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8. Produção de frutosiltransferase por Rhodotorula sp. / Production of fructosyltransferase by Rhodotorula sp.
The global functional food market is estimated at 33 billion dollars per year and a great number of new functional foods have been introduced continuously to the market. A lot of attention has been given to dietary carbohydrates, in special to the fructooligosaccharides (FOS). FOS present advantageous characteristics such as low calories and stimulating effe
Publicado em: 2007
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9. Imobilização da enzima frutosiltransferase extracelular de Rhodotorula sp. e aplicação na produção de frutooligossacarideos. / Imobilization of extracellular enzyme fructosyltransferase from Rhodotorula sp. and application in fructooligosaccharides production.
Fructooligosaccharides (FOS) production by immobilized enzymes encloses two concepts that have faced great expansion in the last few years: industrial application of enzymes and functional foods. The option for enzyme immobilization is mainly induced by the easiness of controlling the reaction and the possibility of reusing the biocatalyst, besides the fact
Publicado em: 2007
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10. Effects of metabolic inhibitors on extracellular fructosyltransferase production in Actinomyces viscosus.
Extracellular fructosyltransferase (levansucrase; EC 2.4.1.10) production in Actinomyces viscosus T14AV was demonstrated to occur concomitantly with cellular growth. The inhibition of both cellular ribonucleic acid and protein synthesis resulted in no further accumulation of enzyme activity. The antibiotic sodium clofibrate differentially inhibited the produ
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11. Role of NADH oxidase in the oxidative inactivation of Streptococcus salivarius fructosyltransferase.
A cell-associated fructosyltransferase produced by Streptococcus salivarius was irreversibly inactivated in a time-dependent manner when resting or permeabilized cell suspensions were incubated with low concentrations (less than 1.0 microM) of copper. In addition to copper, the inactivation was dependent on oxygen and on a fermentable carbon source (endogeno
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12. Metabolism of glycosylsucrose by oral microorganisms and its hydrolysis by Streptococcus salivarius fructosyltransferase.
Resting-cell suspensions of oral microorganisms grown in sucrose were studied for the production of acid from glucosylsucrose and maltosylsucrose. Most oral microorganisms fermented these sugars to only a limited extent. Streptococcus salivarius, however, metabolized glucosylsucrose as well as sucrose. We therefore looked for a specific enzyme in S. salivari