Freezing Semen
Mostrando 25-36 de 42 artigos, teses e dissertações.
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25. Testes funcionais de membrana e índice de prenhez utilizando sêmen crio preservado de tourinhos Tabapuã aos dois anos de idade criados a pasto e previamente selecionados pela CAP.
ABSTRACT According to Brazilian College of Animal Reproduction (BCAR) criteria for post freezing semen classification, 12 young bulls were further selected to evaluate semen quality based on fast (TTRf) and slow (TTRs) termoresistence tests, hyposmotic test (HOST), induced acrossomic reaction by heparin (IAR), percentage of live sperm stained by trypan blue,
Publicado em: 2007
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26. Semen cryopreservation of rainbow trout XX-males. / CriopreservaÃÃo do sÃmen de machos-XX de truta arco-Ãris (Oncorhynchus mykiss)
This study was carried out during July and August of 2005 and 2006. The aim of this study was to improve the cryopreservation techniques for semen cryopreservation of sex-reversed rainbow trout males (XX-males). The first session of experiments, only semen collected under abdominal massage was used. In experiment 1, four semen extenders (glucose 5,4%, NaCl 0
Publicado em: 2007
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27. Colheita, análise e criopreservação de sêmen de uma espécie modelo de primata neotropical, Sagui-de-Tufo-Branco (Callithrix jacchus) / Collection, analysis and cryopreservation of semen from a model species, the common marmoset (Callithrix jacchus)
The Callithrix jacchus can be used as a model species for other Neotropical primates species, mainly Callithriquids, for reproductive studies. Semen samples were collected with the following objectives: to improve the penile vibrostimulation technique, validate sperm evaluation techniques for field work, analyse the semen characteristics from animals at 2 co
Publicado em: 2007
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28. Criopreservação de sêmen de galos / Roosters sperm cryopreservation
Adding extenders to poultry semen is currently used in artificial insemination programs to optimize the management of genetically superior males. Fresh semen fertility usually declines after 1 hour of collection, raising the need to use diluents and low temperatures to store semen for longer periods. Low density lipoprotein (LDL), extracted from the egg yolk
Publicado em: 2007
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29. Freezing of boar semen: study of penetrating and nonpenetrating cryoprotectants, freezing methods and freezability molecular marker / Congelamento de sêmen suíno: estudo de crioprotetores intra e extracelulares, metodologias de congelamento e marcador molecular de congelabilidade
Apesar do sêmen suíno congelado estar disponível comercialmente deste 1975, o seu uso tem ocorrido somente em ocasiões específicas, pois, em relação ao uso de sêmen refrigerado, requer duas a três vezes maior número de espermatozóides por dose, o processamento do sêmen é trabalhoso, o tamanho da leitegada é diminuído em um a três leitões por
Publicado em: 2007
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30. Effect of the addition of glycerol in different stages of the cooling on the freezability of stallion semen / Efeito da adição de glicerol em diferentes etapas do resfriamento sobre a congelabilidade do sêmen de garanhões
The objective of the present study was to evaluate the effect on the freezability of equine semen with the addition of glycerol in 0, 35 and 60 minutes the cooling period. Semen from two adult stallions (Mangalarga Marchador) were used, totalizing 11 colections of semen. The evaluation consisted of sperm progressive straight motility, total motility, sperm v
Publicado em: 2007
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31. Ativadores e concentraÃÃes de metanol e dimetilsulfÃxido na qualidade do sÃmen criopreservado de curimba (Prochilodus lineatus) / Activators and concentrations of methanol and dimethyl sulphoxide in quality of cryopreserved sperm of curimba Prochilodus lineatus
This trial was carried out from November 2005 to January 2006, in order to compare toxicity and the effect of the addition of cryoprotectant and activator solutions to curimba Prochilodus lineatus semen, before and after freezing. Semen samples of 10 breeders were analyzed and diluted in eight cryoprotectants solutions (1:4). These were composed for methanol
Publicado em: 2006
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32. Resfriamento e criopreservaÃÃo do sÃmen de curimba Prochilodus lineatus (Valenciennes, 1836) / Semen cool storage and cryopreservation of curimba Prochilodus lineatus (VALENCIENNES, 1836)
Curimba (Prochilodus lineatus) is a species of native fish of the Grande river. This species is commercially important for being supplied as food to other ecological or commercial species, of interests, during the fingerling production. Moreover, it is protein source of high quality for marginal and devoid populations. The objective of this work was to devel
Publicado em: 2006
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33. Efeito de diferentes concentrações de plasma seminal na criopreservação de sêmen eqüino
One of the major limiting factors of equine frozen semen is the low fertility obtained. During freezing process spermatozoa undergoes a series of damages such as changes in membrane components, premature capacitation, chromatin damages and oxidative stress. Sperm cells are very susceptible to oxidative stress due to high concentration of polyunsaturated fatt
Publicado em: 2006
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34. Effect of different cooling rates, equilibration times and permeating cryoprotectants on deep-freezing of buck spermatozoa / Efeito de diferentes curvas de resfriamento, tempos de equilíbrio e crioprotetores permeáveis no congelamento de espermatozóides de caprinos
O objetivo deste estudo foi avaliar o efeito de diferentes protocolos de resfriamento, tempos de equilíbrio e crioprotetores permeáveis sobre as características de motilidade e integridade de membrana de espermatozóides caprinos criopreservados. Para tal, foram delineados três experimentos. No experimento 1, avaliou-se o efeito de diferentes protocolos
Publicado em: 2006
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35. Effect of external cryoprotectants on the in vitro viability of frozen spermatozoa of SWISS-ALBINA and BALB/c mice (Mus musculus). / Efeito de crioprotetores externos sobre a viabilidade in vitro de espermatozóides congelados de camundongos (Musmusculus) das linhagens SWISSALBINA e BALB/c.
The genetic progress obtained with trangenesis required the development and consolidation of reproductive techniques such as in vitro embryo production and gamete cryopreservation. With the diversity of new models and genetic lines, it became critical to optimize the use of experimental techniques of cryopreservation of semen in different animal species. The
Publicado em: 2006
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36. Efeito de diferentes tempos de equilíbrio, taxas de congelamento e concentrações espermáticas na fertilidade do sêmen eqüino / Effect of different times of balance, freezing rates and concentrations espermatics in the fertility of the semen equine
O objetivo deste estudo foi avaliar a eficiência de diferentes tempos de equilíbrio, as curvas de congelamento e descongelamento e a concentração espermática por dose inseminante sobre a fertilidade do sêmen eqüino. No Experimento 1, foram utilizados cinco ejaculados de três garanhões da raça Mangalarga Marchador, sendo o grupo experimental dividid
Publicado em: 2006