Fluorescence Anisotropy
Mostrando 13-24 de 153 artigos, teses e dissertações.
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13. Kinetics and molecular dynamics of the dispersion process in two-dimensional systems, flow injection (FI): construction and validation of an experimental apparatus. / Cinética e dinâmica molecular do processo de dispersão bidimensional em sistemas de injeção em fluxo (FI):construção e validação de um aparato experimental.
Este trabalho é constituído de três etapas, a saber: planejamento e construção de um aparato experimental para medidas de fluorescência total (LIF) e depolarização fluorescente (PLF) em sistema de injeção em fluxo (FI); testes operacionais visando otimizar parâmetros experimentais; avaliação fotoquímica/fotofísica da cinética e dinâmica mole
Publicado em: 2002
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14. Fração histonica nuclear rica em histona H5 : isolamento, caracterização, orientação macromolecular e interação com DNA
This thesis deals with structural aspects of an H IND. 5 histone rich fraction ( H IND. 5 RF), as well as its importance in the chromatin structure. H IND. 5 RF was extracted from chicken erythrocyte nuclei. Some intrinsic characteristics of H IND. 5 RF a few differenciating qualities in relation to the H IND. 1 histone of the calf thymus were determined by
Publicado em: 1983
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15. Structural order of lipids and proteins in membranes: evaluation of fluorescence anisotropy data.
The limiting long-time value of fluorescence anisotropy in membranes is correlated with the orientational order parameter, which characterizes the structural anisotropy of membranes. Existing experimental results for diphenylhexatriene in lipid bilayers are evaluated for the order parameter of lipid order. Steady-state measurements of fluorescence anisotropy
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16. Resolution of multiphasic reactions by the combination of fluorescence total-intensity and anisotropy stopped-flow kinetic experiments.
Multiphasic kinetics are often observed in stopped-flow investigations. To characterize further these kinetic phases, we have developed a methodology whereby fluorescence total intensity and anisotropy stopped-flow data can be combined in a single analysis. Fluorescence total intensity and anisotropy are highly interrelated and contain two very complementary
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17. Molecular dynamics simulations of fluorescence polarization of tryptophans in myoglobin.
The fluorescence of heme proteins is influenced by energy transfer from the excited tryptophan to the heme. Molecular dynamics simulations of the tryptophan and heme motions in sperm whale myoglobin were used to calculate the fluorescence intensity and anisotropy decays. The side chains underwent both small rapid orientational fluctuations and large infreque
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18. Single-molecule fluorescence spectroscopy of enzyme conformational dynamics and cleavage mechanism
Fluorescence resonance energy transfer and fluorescence polarization anisotropy are used to investigate single molecules of the enzyme staphylococcal nuclease. Intramolecular fluorescence resonance energy transfer and fluorescence polarization anisotropy measurements of fluorescently labeled staphylococcal nuclease molecules reveal distinct patterns of fluct
The National Academy of Sciences.
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19. Quantitative analysis of polymerase chain reaction using anisotropy ratio and relative hydrodynamic volume of fluorescence polarization method.
The method based on the combination of polymerase chain reaction (PCR) and fluorescence polarization is presented. A targeted DNA was amplified with a 5'-fluorescein labeled primer, using a 256 bp DNA fragment of stx2 gene in Escherichia coli O157:H7 (188-443 bp) as a template. The fluorescence anisotropy of the 5'-fluorescein labeled primer increased upon t
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20. Fraction of myosin cross-bridges bound to actin in active muscle fibers: estimation by fluorescence anisotropy measurements.
Time-resolved and steady-state fluorescence anisotropy measurements from fluorescence-labeled myosin cross-bridges in single glycerinated skeletal muscle fibers in rigor, relaxed, MgADP-induced, and contracting states have been made in order to estimate the fraction of actin-bound cross-bridges in active muscle. When the plane of polarization of the excitati
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21. Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy.
Chromatin core particles containing 146 base pairs of DNA have been found to undergo a single defined transition below 10 mM ionic strength as studied by both sedimentation velocity and tyrosine fluorescence anisotropy. A method is described for the preparation of such core particles from chicken erythrocytes with greater than 50% yield.
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22. Impact of Emission Anisotropy on Fluorescence Spectroscopy and FRET Distance Measurements
The objective of this report is to provide a practical and improved method for estimating Förster resonance energy transfer distance measurement error due to unknown angles in the dipole orientation factor based on emission anisotropy measurements. We improve on the method of Dale et al. (1979), which has minor mistakes and is frequently interpreted in over
The Biophysical Society.
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23. Decay of Fluorescence Emission Anisotropy of the Ethidium Bromide-DNA Complex Evidence for an Internal Motion in DNA
Evidence for an internal oscillatory Brownian motion in the DNA helix is obtained from the measurement of the decay of the fluorescence emission anisotropy of the ethidium bromide-DNA complex. The amplitude of the oscillation is found to be equal to 35° and the relaxation time equal to 28 nanoseconds.
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24. The orientation of eosin-5-maleimide on human erythrocyte band 3 measured by fluorescence polarization microscopy.
The dominant motional mode for membrane proteins is uniaxial rotational diffusion about the membrane normal axis, and investigations of their rotational dynamics can yield insight into both the oligomeric state of the protein and its interactions with other proteins such as the cytoskeleton. However, results from the spectroscopic methods used to study these