Quantitative analysis of polymerase chain reaction using anisotropy ratio and relative hydrodynamic volume of fluorescence polarization method.
AUTOR(ES)
Ye, B C
RESUMO
The method based on the combination of polymerase chain reaction (PCR) and fluorescence polarization is presented. A targeted DNA was amplified with a 5'-fluorescein labeled primer, using a 256 bp DNA fragment of stx2 gene in Escherichia coli O157:H7 (188-443 bp) as a template. The fluorescence anisotropy of the 5'-fluorescein labeled primer increased upon the polymerization through Taq polymerase. The conversion of primer to PCR product was quantitatively monitored by anisotropy ratio and relative hydrodynamic volume. This system was also applied to the determination of E.coli O157:H7.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=147744Documentos Relacionados
- Analysis of rev gene function on human immunodeficiency virus type 1 replication in lymphoid cells by using a quantitative polymerase chain reaction method.
- Amplification and sequence analysis of DNA flanking integrated proviruses by a simple two-step polymerase chain reaction method.
- Detection of Xanthomonas campestris pv. citri by the polymerase chain reaction method.
- Polymerase chain reaction products containing 5-methyldeoxycytidine: a microplate immunoquantitation method.
- Theoretical uncertainty of measurements using quantitative polymerase chain reaction.