Flow Cytometer
Mostrando 13-24 de 63 artigos, teses e dissertações.
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13. Effects of chronic and prolonged solar radiation in immunologic system of fishermen from Recife, Brazil / Efeitos da radiação solar crônica e prolongada sobre o sistema imunológico de pescadores do Recife
Introduction: The ultraviolet radiation effects on the human immunological system are highly complex, disturbing some components of immune response. Objective: The objective of this study was to evaluate the clinical, histopathological and immunological effects from solar radiation in male fishermen with more than 10 years of uninterrupted activity. Methods:
Publicado em: 2007
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14. Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)
In order to evaluate the taeniosis-cysticercosis complex in a population of a peasants' settlement, located at Teodoro Sampaio, state of São Paulo, Brazil (longitude 52° 36'12 ", latitude 22° 17'12 ") a series of laboratory markers were determined. After signing an informed consent, participants answered a standardized questionnaire. To determine anti-Tae
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-02
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15. Estudo da expressão de Antígenos Aberrantes em plasmócitos de portadores de Mieloma Múltiplo através de Citometria de Fluxo Mul-tiparamétrica / Detection of aberrant antigens in multiple myeloma cells by multiparametric flow cytometry.
The aims of this study were to determine aberrant antigens (AA) in MM cells and to evaluate their prognostic value and frequency. Methods : bone marrow plasma cells of an homogeneous cohort of 30 newly diagnosed patients with MM were studied. As normal control, bone marrow plasma cells from 4 healthy marrow do-nors were also evaluated. Surface and cytoplasmi
Publicado em: 2006
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16. Efeito da despolarizaÃÃo da membrana plasmÃtica na modulaÃÃo da apoptose de neutrÃfilos mediada por lipopolissacarÃdeo
Among its many biologic activities, extracellular calcium plays a key role in the maintenance of plasma membrane polarization. The effect of membrane depolarization induced by low extracellular calcium concentration or ouabain in neutrophil (PMN) apoptosis and Caspase-3 expression are evaluated. PMN were isolated from blood of healthy lab volunteers by densi
Publicado em: 2003
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17. Evaluation of a method for counting absolute numbers of cells with a flow cytometer.
We evaluated a method for performing absolute cell counts of lymphocyte populations with a flow cytometer. In this method, TruCount, test tubes that contain a known number of brightly fluorescent polystyrene beads are provided by the manufacturer. Whole anticoagulated blood is accurately pipetted into the tubes and mixed with fluorochrome-labeled monoclonal
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18. Unsatisfactory Performance of Flow Cytometer UF-100 and Urine Strips in Predicting Outcome of Urine Cultures
UF-100 flow cytometer and urine strip results were cross-interpreted to predict culture outcomes. The best negative predictive value was obtained with bacteria at ≥1,000/μl, white blood cells at ≥20/μl, or leukocyte esterase positivity. Nine of 24 false negatives were clinically significant. Thus, UF-100 and urine strip results do not accurately predic
American Society for Microbiology.
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19. The flow cytometer.
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20. Selective electrofusion of conjugated cells in flow.
Using a modified flow cytometer we have induced electrofusion of K562 and L1210 cells in flow. The two cell types are stained with two different fluorescent membrane probes, DiO and DiI, to facilitate optical recognition, and then coupled through an avidin-biotin bridge. In the flow cytometer, the hydrodynamically focused cells and cell pairs are first optic
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21. Pathology—Important Advances in Clinical Medicine: The Flow Cytometer in the Clinical Laboratory
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22. Flow cytometry in diagnosis and management of large fetomaternal haemorrhage.
AIMS--To evaluate an indirect immunofluorescence flow cytometry technique in a series of patients with large fetomaternal haemorrhage (FMH). METHODS--Patient samples identified by Kleihauer testing in local laboratories as having FMH > 4 ml were sent for flow cytometric analysis. In a proportion of cases the mothers received anti-D immunoglobulin prophylaxis
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23. Simultaneous Detection of Multiplex-Amplified Human Immunodeficiency Virus Type 1 RNA, Hepatitis C Virus RNA, and Hepatitis B Virus DNA Using a Flow Cytometer Microsphere-Based Hybridization Assay
The feasibility of performing a multiplex assay for the detection of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNAs and hepatitis B virus (HBV) DNA is demonstrated. This assay is based (i) on the coamplification of a 142-bp fragment from the gag region of the HIV-1 genome and a 142-bp HIV-1 quantitation standard fragment, a 244-
American Society for Microbiology.
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24. Effects of Flow Cytometric Analysis on Morphology and Viability of Fragile Phytoplankton †
We assessed damage done to especially delicate marine phytoplankton cells by passage through a Coulter Epics V flow cytometer. The cells did not distort or lyse after exposure to fluidics or to laser light to 1,000 mW. The cells did sustain damage evidenced by temporary growth rate depressions. The four clones tested eventually resumed control growth rates a