Flow Cytometer
Mostrando 1-12 de 63 artigos, teses e dissertações.
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1. Validation of the single-platform ISHAGE protocol for enumeration of CD34+ hematopoietic stem cells in umbilical cord blood in a Brazilian center
Abstract Background This study aims to validate the single-platform method for enumeration of CD34+ cells, by comparing the performance of two different commercial kits, as well as to evaluate the efficiency of the AccuriTM C6 cytometer in providing direct counts of absolute cell numbers. Method We evaluated 20 samples from umbilical cord blood (UCB), comp
Hematology, Transfusion and Cell Therapy. Publicado em: 2022
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2. Aquios CL flow cytometer performance in the automated quantification of lymphocyte subpopulations
RESUMO A citometria de fluxo (CF) é uma ferramenta importante para diagnóstico, prognóstico e acompanhamento terapêutico de diversas neoplasias hematológicas. Além disso, possibilita a quantificação das subpopulações linfocitárias (SPL) para assistência diagnóstica e monitoramento das imunodeficiências primárias e adquiridas por meio das expre
J. Bras. Patol. Med. Lab.. Publicado em: 02/09/2019
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3. A new heparan sulfate from the mollusk Nodipecten nodosus inhibits merozoite invasion and disrupts rosetting and cytoadherence of Plasmodium falciparum
BACKGROUND Despite treatment with effective antimalarial drugs, the mortality rate is still high in severe cases of the disease, highlighting the need to find adjunct therapies that can inhibit the adhesion of Plasmodium falciparum-infected erythrocytes (Pf-iEs). OBJECTIVES In this context, we evaluated a new heparan sulfate (HS) from Nodipecten nodosus
Mem. Inst. Oswaldo Cruz. Publicado em: 06/06/2019
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4. Two new mixed-ligand coordination polymers based on multi-N chelating ligand inhibit YAP expression and induce caspase-mediated spinal tumor cell apoptosis
Two new coordination polymers [Zn (bdc)(bpybzimH2)](DMF)0.5 (1, H2bdc=1,4-dicarboxybenzene, bpybzimH2=6,6′-bis-(1H-benzoimidazol-2-yl)-2,2′-bipyridine, DMF=N,N-dimethylformamide) and [Co (bpybzimH2)(sbc)]H2O (2, H2sbc=4-mercaptobenzoic acid) have been successfully prepared under solvothermal conditions using the multi-N chelating organic ligand bpybzimH2
Braz J Med Biol Res. Publicado em: 16/05/2019
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5. Aldefluor protocol to sort keratinocytes stem cells from skin
Abstract Purpose: To investigate the use Aldefluor® and N, N - Dimethylaminobenzaldehyde (DEAB) to design a protocol to sort keratinocyte stem cells from cultured keratinocytes from burned patients. Methods: Activated Aldefluor® aliquots were prepared and maintained at temperature between 2 to 8°C, or stored at -20°C. Next, the cells were collected fo
Acta Cir. Bras.. Publicado em: 2017-11
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6. Aerobic training modulates T cell activation in elderly women with knee osteoarthritis
Osteoarthritis of the knee (kOA) is a disease that mainly affects the elderly and can lead to major physical and functional limitations. However, the specific effects of walking, particularly on the immune system, are unknown. Therefore, this study aimed to analyze the effect of 12 weeks of walking (3×/week) on the leukocyte profile and quality of life (QL)
Braz J Med Biol Res. Publicado em: 03/11/2016
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7. Lactobacillus rhamnosus could inhibit Porphyromonas gingivalis derived CXCL8 attenuation
ABSTRACT An increasing body of evidence suggests that the use of probiotic bacteria is a promising intervention approach for the treatment of inflammatory diseases with a polymicrobial etiology. P. gingivalis has been noted to have a different way of interacting with the innate immune response of the host compared to other pathogenic bacteria, which is a rec
J. Appl. Oral Sci.. Publicado em: 2016-02
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8. Normal lymphocyte immunophenotype in an elderly population
OBJECTIVE: The aim of this work was to evaluate the lymphocyte immunophenotype in an elderly population.METHODS: This study enrolled 35 over 60-year-old volunteers and a control group composed of 35 young adults. The study included elderly without diseases that might affect the functioning of the immune system. These individuals were consulted by doctors and
Rev. Bras. Hematol. Hemoter.. Publicado em: 2014-06
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9. Gamma-tocotrienol modulation of senescence-associated gene expression prevents cellular aging in human diploid fibroblasts
OBJECTIVE: Human diploid fibroblasts undergo a limited number of cellular divisions in culture and progressively reach a state of irreversible growth arrest, a process termed cellular aging. The beneficial effects of vitamin E in aging have been established, but studies to determine the mechanisms of these effects are ongoing. This study determined the molec
Clinics. Publicado em: 2012
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10. Determinação da porcentagem de espermatozóides portadores de cromossomos X e Y no sêmen sexado mediante PCR em tempo real / Determination of the proportion of X- and Y- bearing spermatozoa in bovine sexed semen by real time PCR
There are different biotechnologies to apply to increase the animal production nowadays, reproduction techniques are including. The artificial insemination (AI), for example, is a technique very used during many years ago and it is used at extensive production. The aim of this technique is to increase the numbers of pregnancies with just one ejaculation what
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 01/09/2011
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11. Avaliação das populações de linfócitos produtores de IFN-g e IL-17 em pacientes sépticos e relação com o desfecho clínico. / Evaluation of lymphocyte populations producing IFN-g and IL-17 in septic patients and relation to clinical outcome.
The inflammatory response is modulated during sepsis and up or down regulation of cell activity depends on cells and functions evaluated. IFN-g and IL-17 are cytokines characteristics of lymphocyte subsets Th1 and Th17, respectively, and play an important role in immune response, linking innate and adaptative immunity. Objective: To evaluate the presence of
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 28/07/2010
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12. Impact study of fibropapillomatosis in Chelonia mydas (LINNAEUS, 1758) (Testudines, Cheloniidae) / Estudo do impacto da fibropapilomatose em Chelonia mydas (LINNAEUS, 1758) (Testudines, Cheloniidae)
Chelonia mydas, denominada tartaruga verde, é uma tartaruga marinha que freqüenta o litoral brasileiro para alimentação e nidificação e é considerada em perigo de extinção pela IUCN (2006). A fibropapilomatose, doença caracterizada por tumores cutâneos benignos (fibropapilomas), é uma das mais importantes ameaças à sobrevivência dessa espécie
Publicado em: 2007