Fast Fashion
Mostrando 13-24 de 41 artigos, teses e dissertações.
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13. Protein folding from a highly disordered denatured state: The folding pathway of chymotrypsin inhibitor 2 at atomic resolution
Previous experimental and theoretical studies have produced high-resolution descriptions of the native and folding transition states of chymotrypsin inhibitor 2 (CI2). In similar fashion, here we use a combination of NMR experiments and molecular dynamics simulations to examine the conformations populated by CI2 in the denatured state. The denatured sta
The National Academy of Sciences.
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14. Ca2+- and K+-dependent communication between central nervous system myelinated axons and oligodendrocytes revealed by voltage-sensitive dyes.
The interactions between myelinated axons and surrounding glia cells, in rat optic nerve, were investigated by optical recording with voltage-sensitive dyes. Electrical stimulation of the nerve evoked an optical signal revealing two clearly distinct components: a fast propagating component, corresponding to the compound action potential, and a prominent slow
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15. Transgenic AEQUORIN reveals organ-specific cytosolic Ca2+ responses to anoxia and Arabidopsis thaliana seedlings.
Using the transgenic AEQUORIN system, we showed that the cotyledons and leaves of Arabidopsis thaliana seedlings developed a biphasic luminescence response to anoxia, indicating changes in cytosolic Ca2+ levels. A fast and transient luminescence peak occurred within minutes of anoxia, followed by a second, prolonged luminescence response that lasted 1.5 to 4
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16. Susceptibility of p53 Unstructured N Terminus to 20 S Proteasomal Degradation Programs the Stress Response*♦
The N-terminal transcription activation domain of p53 is intrinsically unstructured. We show in vitro and in vivo that this domain initiates p53 degradation by the 20 S proteasome in a ubiquitin-independent fashion. The decay of metabolically labeled p53 follows biphasic kinetics with an immediate fast phase that is ubiquitin-independent and a second slower
American Society for Biochemistry and Molecular Biology.
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17. Phencyclidine block of calcium current in isolated guinea-pig hippocampal neurones.
1. Phencyclidine (PCP) block of Ca2+ channel current in enzymatically dissociated neurones from the CA1 region of the adult guinea-pig hippocampus was studied using whole-cell voltage clamp techniques. Ca2+ channel current was recorded with 3 mM-Ba2+ as the charge carrier. Na+ currents were blocked with tetrodotoxin and K+ currents were eliminated by using t
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18. Tracking the Evolution of the SARS Coronavirus Using High-Throughput, High-Density Resequencing Arrays
Mutations in the SARS-Coronavirus (SARS-CoV) can alter its clinical presentation, and the study of its mutation patterns in human populations can facilitate contact tracing. Here, we describe the development and validation of an oligonucleotide resequencing array for interrogating the entire 30-kb SARS-CoV genome in a rapid, cost-effective fashion. Using thi
Cold Spring Harbor Laboratory Press.
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19. Involvement of methyltransferase-activating protein and methyltransferase 2 isoenzyme II in methylamine:coenzyme M methyltransferase reactions in Methanosarcina barkeri Fusaro.
The enzyme systems involved in the methyl group transfer from methanol and from tri- and dimethylamine to 2-mercaptoethanesulfonic acid (coenzyme M) were resolved from cell extracts of Methanosarcina barkeri Fusaro grown on methanol and trimethylamine, respectively. Resolution was accomplished by ammonium sulfate fractionation, anion-exchange chromatography,
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20. Two HLA-linked loci controlling the fourth component of human complement.
An electrophoretic polymorphism of the fourth component of human complement (C4) is described. Three patterns of bands of C4 were observed in EDTA plasma from a panel of unrelated blood donors and family members by using the technique of immunofixation electrophoresis. These patterns consisted of four fast-moving anodal bands (F), four slow-moving cathodal b
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21. A voltage-dependent role for K+ in recovery from C-type inactivation.
Recovery from C-type inactivation of Kv1.3 can be accelerated by the binding of extracellular potassium to the channel in a voltage-dependent fashion. Whole-cell patch-clamp recordings of human T lymphocytes show that Ko+ can bind to open or inactivated channels. Recovery is biphasic with time constants that depend on the holding potential. Recovery is also
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22. Synaptotagmin IV regulates glial glutamate release
Calcium-binding synaptotagmins (Syts) are membrane proteins that are conserved from nematode to human. Fifteen Syts (Syts I–XV) have been identified in mammalian species. Syt I has been well studied and is a candidate for the Ca2+-sensor that triggers evoked exocytosis underlying fast synaptic transmission. Whereas the functions of the other Syts are uncle
National Academy of Sciences.
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23. The fade of the response to acetylcholine at the rabbit isolated sino-atrial node.
1. The effect of acetylcholine (ACh) on the frequency of spontaneous action potentials, recorded with glass microelectrodes from small preparations of the sino-atrial node of the rabbit, has been investigated. 2. On application of ACh there was a rapid increase in cycle length (the interval between successive action potentials) but then, despite the continue
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24. Influence of study design in assessing food effects on absorption of erythromycin base and erythromycin stearate.
We performed a series of six single-dose and multiple-dose studies to evaluate the effect of food on the absorption of erythromycin base and erythromycin stearate. When we used a single-dose design, we found that an unprotected erythromycin base preparation was absorbed extensively if a prolonged fast preceded administration of the drug. A shorter faster per