Expressed Sequence Tag
Mostrando 1-12 de 371 artigos, teses e dissertações.
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1. Improved antifungal activity of barley derived chitinase I gene that overexpress a 32 kDa recombinant chitinase in Escherichia coli host
Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I
Braz. J. Microbiol.. Publicado em: 2018-06
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2. Evaluation of total flavonoid content and analysis of related EST-SSR in Chinese peanut germplasm
Abstract As important antioxidants and secondary metabolites in peanut seeds, flavonoids have great nutritive value. In this study, total flavonoid contents (TFC) were determined in seeds of 57 peanut accessions from the province of Hebei, China. A variation of 0.39 to 4.53 mg RT g-1 FW was found, and eight germplasm samples containing more than 3.5 mg RT g-
Crop Breed. Appl. Biotechnol.. Publicado em: 2017-09
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3. Identification and validation of novel EST-SSR markers in olives
ABSTRACT: The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) marke
Sci. agric. (Piracicaba, Braz.). Publicado em: 2017-06
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4. Differentially expressed genes and miRNA identification in pig skeletal muscle / Identificação de genes diferencialmente expressos e miRNAs em músculo esquelético de suínos
The pig (Sus scrofa) is considered an important animal for meat production. This interest revolves around the potential for muscle growth, which usually is associated with certain characteristics during prenatal myogenesis. To study the genes responsible for these characteristics, expressed sequence tags (EST) provide direct information about the transcripto
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 25/07/2011
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5. Proteômica e transcriptômica aplicadas ao estudo da variabilidade do veneno de Bothrops jararaca (serpentes:viperidae) / Proteomics and transcriptomics applied to the study of Bothrops jararaca venom variability (Serpentes: Viperidae)
Estudos prévios demonstraram que as atividades biológicas do veneno da serpente Bothrops jararaca sofrem significantes modificações ontogenéticas. Neste estudo é apresentada uma análise comparativa do proteoma, peptidoma e transcriptoma da glândula de veneno de filhotes e adultos de B. jararaca, correlacionando os resultados obtidos com algumas carac
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 30/05/2011
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6. Métodos estatísticos aplicados à análise de dados de etiqueta de sequência expressa / Statistical methods applied to expressed sequence tag data analisys
Pesquisas de Expressed Sequence Tags (ESTs) são uma ferramenta fundamental para identificação de genes em estudos de seqüenciamento de vários organismos. Dado uma amostra preliminar de EST de uma certa biblioteca de cDNA, vários problemas estatísticos de predição podem surgir. Em particular, é de interesse calcular o número de genes, Δ(t), qu
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/02/2011
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7. Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library fro
Genetics and Molecular Biology. Publicado em: 2011
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8. A high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica.
Polyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous exp
PLANT PHYSIOLOGY. Publicado em: 2011
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9. A high-throughput data mining of single nucleotide polymorphisms in Coffea species expresed sequence tags suggests differential homeologous gene expression in the allotetrapoloid Coffea arabica.
Polyploidization constitutes a common mode of evolution in flowering plants. This event provides the raw material for the divergence of function in homeologous genes, leading to phenotypic novelty that can contribute to the success of polyploids in nature or their selection for use in agriculture. Mounting evidence underlined the existence of homeologous exp
PLANT PHYSIOLOGY. Publicado em: 2011
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10. Exploiting a wheat EST database to assess genetic diversity
Expressed sequence tag (EST) markers have been used to assess variety and genetic diversity in wheat (Triticum aestivum). In this study, 1549 ESTs from wheat infested with yellow rust were used to examine the genetic diversity of six susceptible and resistant wheat cultivars. The aim of using these cultivars was to improve the competitiveness of public wheat
Genetics and Molecular Biology. Publicado em: 12/11/2010
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11. Unravelling MADS-box gene family in Eucalyptus spp. : a starting point to an understanding of their developmental role in trees
MADS-box genes encode a family of transcription factors which control diverse developmental processes in flowering plants ranging from root to flower and fruit development. Members of the MADS-box gene family share a highly conserved sequence of approximately 180 nucleotides that encodes a DNA-binding domain. We used bioinformatics tools to investigate the i
Publicado em: 2010
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12. Survey of glycine-rich proteins (GRPs) in the Eucalyptus expressed sequence tag database
The occurrence of quasi-repetitive glycine-rich peptides has been reported in different organisms. Glycine-rich regions are proposed to be involved in protein-protein interactions in some mammalian protein families. In plants, a set of glycine-rich proteins (GRPs) was characterized several years ago, and since then a wealth of new GRPs have been identified.
Publicado em: 2010