Expressed Sequence Tag
Mostrando 13-24 de 371 artigos, teses e dissertações.
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13. Mutations in retrotransposon AtCOPIA4 compromises resistance to Hyaloperonospora parasitica in Arabidopsis thaliana
Retrotransposons (RTEs) are a principal component of most eukaryotic genomes, representing 50%-80% of some grass genomes. RTE sequences have been shown to be preferentially present in disease resistance gene clusters in plants. Arabidopsis thaliana has over 1,600 annotated RTE sequences and 56 of these appear to be expressed because of the exact expressed se
Genetics and Molecular Biology. Publicado em: 11/12/2009
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14. Prokaryotic expression of a novel mouse pro-apoptosis protein PNAS-4 and application of its polyclonal antibodies
Mouse PNAS-4 (mPNAS-4) has 96% identity with human PNAS-4 (hPNAS-4) in primary sequence and has been reported to be involved in the apoptotic response to DNA damage. However, there have been no studies reported of the biological functions of mPNAS-4. In studies conducted by our group (unpublished data), it was interesting to note that overexpression of mPNAS
Brazilian Journal of Medical and Biological Research. Publicado em: 2008-06
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15. Citrus plastid-related gene profiling based on expressed sequence tag analyses
Plastid-related sequences, derived from putative nuclear or plastome genes, were searched in a large collection of expressed sequence tags (ESTs) and genomic sequences from the Citrus Biotechnology initiative in Brazil. The identified putative Citrus chloroplast gene sequences were compared to those from Arabidopsis, Eucalyptus and Pinus. Differential expres
Genetics and Molecular Biology. Publicado em: 2007
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16. A genetic framework for flowering-time pathways in Citrus spp.
Floral transition is one the most drastic changes occurring during the life cycle of a plant. The shoot apical meristem switches from the production of leaves with associated secondary shoot meristems to the production of flower meristems. This transition is abrupt and generally irreversible, suggesting it is regulated by a robust gene regulatory network cap
Genetics and Molecular Biology. Publicado em: 2007
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17. Comparative linkage mapping of Oryza glumaepatula and Oryza sativa interspecific crosses based on microsatellite and expressed sequence tag markers
Molecular linkage maps representing the rice genome have been an important tool for breeding programs because they allow the elucidation of polygenic traits and are an efficient tool for monitoring wild introgressions in interspecific crosses. Common markers among rice genetic maps are important in defining the homology of chromosomes and the synteny between
Genetics and Molecular Biology. Publicado em: 2007
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18. Differential expression of genes identified from Poncirus trifoliata tissue inoculated with CTV through EST analysis and in silico hybridization
Citrus is the most important fruit crop in Brazil and Citrus tristeza virus (CTV) is considered one of the most important pathogens of citrus. Most citrus species and varieties are susceptible to CTV infection. However, Poncirus trifoliata, a close relative of citrus, is resistant to the virus. In order to better understand the responses of citrus plants to
Genetics and Molecular Biology. Publicado em: 2007
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19. Towards the identification of flower-specific genes in Citrus spp
Citrus sinensis is a perennial woody species, for which genetic approaches to the study of reproductive development are not readily amenable. Here, the usefulness of the CitEST Expressed Sequence Tag (EST) database is demonstrated as a reliable new resource for identifying novel genes exclusively related to Citrus reproductive biology. We performed the analy
Genetics and Molecular Biology. Publicado em: 2007
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20. Nitrogen assimilation in Citrus based on CitEST data mining
Assimilation of nitrate and ammonium are vital procedures for plant development and growth. From these primary paths of inorganic nitrogen assimilation, this metabolism integrates diverse paths for biosynthesis of macromolecules, such as amino acids and nucleotides, and the central intermediate metabolism, like carbon metabolism and photorespiration. This pa
Genetics and Molecular Biology. Publicado em: 2007
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21. Expressão de uricase de Bacillus subtilis em escherichia coli
Bacillus subtilis URICASE EXPRESSION IN Escherichia coli Uricase, an enzyme that converts uric acid into allantoin, is commonly used in commercial kits for uric acid detection. Aiming to produce this enzyme in industrial scale using Genetic Engineering techniques, the uricase gene of Bacillus subtilis subtilis was cloned and expressed in Escherichia coli. In
Publicado em: 2007
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22. Caracterização de uma aldo-ceto redutase relacionada a patogenicidade de Xylella fastidiosa / Characterization of a pathogenicity related Aldo-keto reductase from Xylella fastidiosa
The Xylella fastidiosa genome program generated a large number of gene sequences that belong to pathogenicity, virulence and adaptation categories from this important plant pathogen. One of these genes (XF 1729) was described in the genome annotation as being a phenylacetaldehyde dehydrogenase. However, the XF 1729 primary sequence analysis showed similariti
Publicado em: 2007
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23. Estudo de dois grupos de elementos de cana-de-açúcar homológos à superfamília hAT de transposons / Studies on hAT-like transposases in sugarcane superfamily
Transposable elements (TEs) are mobile genetic sequences. Their mutagenic capacity makes them important sources of variation in the genomes. These elements have another important evolutionary role as donors of functional protein domains in the formation of new genes. 276 cDNA clones homologous to TEs were previously identified in the Brazilian Sugarcane Expr
Publicado em: 2007
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24. Descovery and study of genes involved in immune response against gastrointestinal nematodes in cattle / Descoberta e estudo de genes envolvidos na resposta a endoparasitas gastrintestinais em bovinos
Este trabalho teve por objetivo identificar e estudar a expressão de genes envolvidos na resposta imune a endoparasitas gastrintestinais em bovinos. Contagem periódica de ovos por grama de fezes (OPG) e análises de coproculturas foram realizadas para identificar animais resistentes e susceptíveis a endoparasitas gastrintestinais. A contagem de OPG permit
Publicado em: 2007