Euglena
Mostrando 61-72 de 259 artigos, teses e dissertações.
-
61. Lipid Metabolism of Manganese-deficient Algae: I. Effect of Manganese Deficiency on the Greening and the Lipid Composition of Euglena Gracilis Z 1
The growth of photoautotrophic Euglena gracilis Z is strongly inhibited by manganese deficiency, whereas chlorophyll formation is not appreciably affected. The galactosyldiglyceride content of the manganese-deficient photo-autotrophic Euglena was about 40% lower on the basis of either chlorophyll content or dry weight. When dark-grown cultures of Euglena wer
-
62. Serological Characterization of the Glycolate-oxidizing Enzymes from Tobacco, Euglena gracilis, and a Yellow Mutant of Chlorella vulgaris
An antiserum to tobacco glycolate oxidase has been prepared by injection of the purified enzyme into rabbits. Double gel diffusion tests between the antiserum and purified antigen and also with a crude tobacco preparation gave a single immunoprecipitation band. Crude extracts of Euglena gracilis Z Klebs, containing glycolate dehydrogenase, and of Chlorella v
-
63. Light Activation of Mg2+-dependent Adenosine 5′-Triphosphatase in Isolated Euglena Chloroplasts 1
Enhancement of Mg2+-dependent ATPase activity in Euglena gracilis chloroplasts by light in the presence of a sulfhydryl compound has been demonstrated. A number of uncouplers and energy transfer inhibitors were studied for their effects on the light enhancement of ATPase activity simultaneously with their effects on photophosphorylation. Results suggest that
-
64. Investigations into the Euglena method for the assay of the vitamin B12 in serum
Details are given of a modified Euglena method for the assay of B12 in serum. Serum in very small quantities is usually inhibitory to the growth of the Euglena. In general, this inhibition is most marked when the organism is growing prolifically. For estimating serum B12 values, a standard curve is used from B12 standards containing 0·01 ml. of serum of low
-
65. A Study of Rubidium Accumulation in Euglena gracillis1
The absorption of Rb+ by Euglena gracillis var. bacillaris was examined, and an active uptake process could be detected within 1 hour. This was established by experiments that included a time course study and metabolic depression by either reducing the temperature or by addition of 2,4-dinitrophenol.
-
66. Short leader sequences may be transferred from small RNAs to pre-mature mRNAs by trans-splicing in Euglena.
Very closely related short sequences are present at the 5' end of cytoplasmic mRNAs in Euglena as evidenced by comparison of cDNA sequences and hybrid-arrested translation experiments. By cloning Euglena gracilis nuclear DNA and isolating the rbcS gene (encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase), we have shown that the sho
-
67. Sequential changes in cell volume distribution during vitamin B12 starvation of Euglena gracilis.
During vitamin B12 starvation of Euglena, a new peak appears in the cell volume distribution. Some cells are inhibited at a unique point in the cell cycle between the initiation of DNA synthesis and nuclear division. The mechanism of inhibition of other cells differs.
-
68. Ribulose Diphosphate Carboxylase from Autotrophic Euglena gracilis
Ribulose 1,5-diphosphate carboxylase (RUDPcase) from autotrophically grown Euglena gracilis was purified to homogeneity as measured by analytical ultracentrifugation, polyacrylamide gel electrophoresis, and immunoprecipitation reactions. The enzyme represented about 9% of total protein and 24% of soluble protein in the autotrophic cell. Light-grown, heterotr
-
69. A New Type of a Multifunctional β-Oxidation Enzyme in Euglena
The biochemical and molecular properties of the β-oxidation enzymes from algae have not been investigated yet. The present study provides such data for the phylogenetically old alga Euglena (Euglena gracilis). A novel multifunctional β-oxidation complex was purified to homogeneity by ammonium sulfate precipitation, density gradient centrifugation, and ion-
American Society of Plant Biologists.
-
70. Isolation of Intact Chloroplasts from Euglena gracilis by Zonal Centrifugation 1
Chloroplasts were separated from Euglena gracilis by zonal centrifugation at low speed in density gradients of Ficoll or dextran. The chloroplasts were intact by the criteria of ultrastructure and their content of ribulose diphosphate carboxylase and soluble protein. The chloroplasts also contained ribosomes and ribosomal RNA uncontaminated by the correspond
-
71. Isolation of Intact Chloroplasts of Euglena gracilis by Isopycnic Sedimentation in Gradients of Silica 12
A technique is described for the isolation of structurally intact and partially active chloroplasts from photoheterotrophically grown Euglena gracilis. The separation of intact chloroplasts from stripped chloroplast membranes and other subcellular particles was achieved by sedimentation in continuous, isosmotic density gradients of Ludox AM, a silica sol.
-
72. Studies of Sulfate Utilization of Algae: 15. Enzymes of Assimilatory Sulfate Reduction in Euglena and Their Cellular Localization 1
Crude extracts of wild-type Euglena grown in the light (WTL) or in the dark (WTD) and a mutant lacking detectable plastid DNA (W3BUL) contain adenosine 5′-phosphosulfate (APS) sulfotransferase. Isotope dilution experiments indicate that adenosine 3′-phosphate 5′-phosphosulfate (PAPS) sulfotransferase is absent.