Euglena
Mostrando 49-60 de 259 artigos, teses e dissertações.
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49. Amino Acid Composition of Bulk Protein of Euglena Grown in Waste Water
The amino acid content of bulk protein in a sewage-grown Euglena sp. was examined. Concentrations of the essential amino acids, threonine, histidine, tryptophan, and valine, were similar to those found in other algae. The concentration of alanine was much higher. Methionine was not found at all, proline only in traces, and other amino acids at low concentrat
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50. Nuclear rDNA in Euglena gracilis: paucity of chromosomal units and replication of extrachromosomal units.
Copy number of chromosomal rDNA units was investigated in two Euglena gracilis wild-type strains. It was established by dot blot analysis that these strains possess about four integrated units per haploid genome. This is the first example of a photosynthetic cell with only a few chromosomal ribosomal genes. In addition to these units, Euglena has 800 to 4000
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51. The Effect of Light on the Synthesis of Mitochondrial Enzymes in Division-synchronized Euglena Cultures
The development of the mitochondrial enzymes fumarase and succinate dehydrogenase has been followed in Euglena cultures division-synchronized by 14-hour light periods alternating with 12-hour dark periods. The activity of both enzymes was unaltered over the light phase, doubled in early dark phase, and thereafter remained constant over the rest of the cycle.
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52. U1 small nuclear RNA and spliceosomal introns in Euglena gracilis
In the flagellated protozoon Euglena gracilis, characterized nuclear genes harbor atypical introns that usually are flanked by short repeats, adopt complex secondary structures in pre-mRNA, and do not obey the GT-AG rule of conventional cis-spliced introns. In the nuclear fibrillarin gene of E. gracilis, we have identified three spliceosomal-type introns tha
The National Academy of Sciences.
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53. Catabolite repression of chloroplast development in Euglena
Two-dimensional gel electrophoresis resolved total cellular protein from Euglena gracilis Klebs var. bacillaris Cori into 640 polypeptides detectable by silver staining. The addition of 84 mM ethanol to dark-grown resting carbon-starved cells increased the relative amounts of 6 polypeptides and decreased the relative amounts of 3 polypeptides. The addition o
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54. Photosynthesis of Euglena gracilis under Cobalamin-Sufficient and -Limited Growing Conditions 1
Cobalamin is essential for growth of Euglena gracilis and photosynthesis. Methylcobalamin in Euglena chloroplasts (Y Isegawa, Y Nakano, S Kitaoka, 1984 Plant Physiol 76: 814-818) functions as a coenzyme of methionine synthetase. The requirement of cobalamin for photosynthesis appeared remarkably high in Euglena grown under the dark-precultured condition. The
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55. The Sites of Transcription and Translation for Euglena Chloroplastic Aminoacyl-tRNA Synthetases
We find that cycloheximide completely blocks the light-induced apearance of Euglena chloroplastic aminoacyl-tRNA synthetases in dark-grown cells of Euglena gracilis var. bacillaris. Streptomycin, on the other hand, has no effect on the light-induction of these organellar enzymes. These observations, together with the finding that an aplastidic mutant (strain
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56. The Effect of 3-(3,4-Dichlorophenyl)-1,1-dimethylurea on Chloroplast Development and Maintenance in Euglena gracilis: I. Ultrastructural Characterization of Light-Grown Cells by the Techniques of Thin Sectioning and Freeze-Etching 1
The ultrastructure of light-grown Euglena gracilis var. bacillaris was examined by the techniques of thin sectioning and freeze-etching. Thin sectioning revealed the typical organelles previously observed in chemically fixed Euglena. In addition to confirming the observations on thin sections, the freeze-etch technique has revealed the presence in E. gracili
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57. Transcriptional origin of Euglena chloroplast tRNAs*
tRNA·DNA hybridization studies indicate that Euglena chloroplast tRNAs are transcriptional products of the chloroplast genome, which contains approximately 26 tRNA cistrons. Hybridization with purified chloroplast tRNAPhe and tRNAAsp shows that the chloroplast genome contains one cistron for each of these two species. No hybridization of chloroplast tRNA wi
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58. A soluble protein is imported into Euglena chloroplasts as a membrane-bound precursor.
The Euglena precursor to the small subunit of ribulose-15-bisphosphate carboxylase/oxygenase (pSSU) is a polyprotein. To determine the transport route from cytoplasm to chloroplast, Euglena was pulse labeled with 35S-sulfate and the organelles were separated on sucrose gradients. After a pulse, pSSU was found in the endoplasmic reticulum (ER) and Golgi appar
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59. Chloroplast and Cytoplasmic Ribosomes of Euglena: Selective Binding of Dihydrostreptomycin to Chloroplast Ribosomes1
Dihydrostreptomycin binds preferentially to chloroplast ribosomes of wild-type Euglena gracilis Klebs var. bacillaris Pringsheim. The Kdiss for the wild-type chloroplast ribosome-dihydrostreptomycin complex is 2 × 10−7 M, a value comparable with that found for the Escherichia coli ribosome-dihydrostreptomycin complex. Chloroplast ribosomes isolated from t
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60. Structure and expression of the Euglena gracilis nuclear gene coding for the translation elongation factor EF-1 alpha.
A cDNA library from the protist Euglena gracilis was used to isolate and sequence an ORF coding for the elongation factor protein EF-1 alpha. The decoded amino acid sequence (MW, 48'515) is to 75-80% identical with other eukaryotic EF-1 alpha sequences but only to 24% identical with the Euglena chloroplast EF-Tu. Homologous DNA probes interact with multiple