Electrophoresis Modification
Mostrando 1-12 de 274 artigos, teses e dissertações.
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1. Complete substitution of the Brazilian endemic clone by other methicillin-resistant Staphylococcus aureus lineages in two public hospitals in Rio de Janeiro, Brazil
Abstract Staphylococcus aureus is an important cause of bloodstream infections. Therefore, the main purpose of this work was to characterize a collection of 139 S. aureus isolates from bloodstream infections in two public hospitals in relation to their antimicrobial susceptibility profile, staphylococcal cassette chromosome mec types, and clonal relationship
Braz J Infect Dis. Publicado em: 2017-03
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2. Emergence of clonal complex 5 (CC5) methicillin-resistant Staphylococcus aureus (MRSA) isolates susceptible to trimethoprim-sulfamethoxazole in a Brazilian hospital
In this study, genotyping techniques including staphylococcal chromosomal cassette mec (SCCmec) typing, pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST) and restriction-modification tests were used to compare the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) isolates recovered at two times within a
Brazilian Journal of Medical and Biological Research. Publicado em: 2012-07
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3. Análise de uma heme oxigenase funcional em Trypanosoma cruzi / Heme oxygenase analysis in Trypanosoma cruzi
Trypanosoma cruzi, the ethiologic agent of Chagas disease, is transmitted through triatomine vectors during their blood-meal on vertebrate host. These hematophagous insects ingest blood about 6 to 12 times its original weight, reaching in a single meal about 10 mM heme bound to hemoglobin. Heme (iron protoporphyrin IX) is an important molecule in metabolism
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 21/02/2011
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4. Análise da variação cariotípica e dos mecanismos de recombinação em leveduras industriais (Saccharomyces cerevisiae) durante o processo de fermentação alcoólica / Analysis of the karyotypic variation and the recombination mechanisms industrial yeast (Saccharomyces cerevisiae) during the alcoholic fermentation process
Brazilian sugar cane ethanol stands out among the energetic alternatives available nowadays. In the fermentation process to produce ethanol it is used the yeast Saccharomyces cerevisiae. One of the most widely adopted strains is the industrial isolate PE-2, currently used by ~30% of Brazilian distilleries, generating ~10% of the worlds ethanol supply. This
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/08/2010
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5. Caracterização das subunidades catalíticas do proteassoma 20S em cepas de Trypanosoma cruzi susceptíveis e resistentes ao benzonidazol
Chagas disease is caused by Trypanosoma cruzi. According to WHO, in Latin America, 16-18 million people is infected with this protozoan. Due the involvement of the proteasome in the cellular biology of the T. cruzi and the low efficiency of the drugs used in the individuals treatment with Chagas disease, we believed that the characterization of the catalytic
Publicado em: 2007
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6. Sequencing, identification and analysis of juvenile Eucalyptus grandis xylem proteins / Sequenciamento, identificação e análise de proteínas do caule de mudas de Eucalyptus grandis
The process of wood formation is an important economical factor for the forestry industry and it is also of ecological importance, although little is known about the proteins involved in wood formation. The sequencing, identification and analysis of proteins provides such information of wood formation. Using proteomics techniques such as two-dimensional gel
Publicado em: 2006
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7. "Padronização e avaliação de métodos moleculares para detecção de oocistos de Cryptosporidium spp. (Apicomplexa: Cryptosporiidae) em amostras fecais: extração de DNA genômico e PCR (reação em cadeia pela polimerase)" / Standardization and evaluation of molecular methods to detect oocysts of Cryptosporidium spp (Apicomplexa: Cryptosporiidae) in faecal samples: extraction of genomic DNA and PCR (polymerase chain reaction).
O protozoário parasita Cryptosporidium parvum tem sido reconhecido como um importante patógeno emergente. Para estudos moleculares, a maioria das técnicas para extração do DNA requer o uso de kits importados para concentrar, romper a parede muito resistente do oocisto e purificar o DNA das matrizes das amostras. O objetivo do estudo foi desenvolver um m
Publicado em: 2004
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8. Estudo de perfil de sensibilidade / ResistÃncia de cepas de Staphylococcus aureus MRSA do Hospital das ClÃnicas de Pernambuco
The appearance of Staphylococcus aureus strains MRSA during the seventies increased rapidly throughout the world. These multiresistant strains are responsable for nosocomial and community infections of difficult treatment. The resistance to meticilin is caused by the modification of a protein bound to penicilins (PBP), PBP2ÂÂ codified as mecA. Abou 40 to 6
Publicado em: 2004
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9. Detecção eletroquímica em eletroforese capilar
This review focuses the development of electrochemical detection systems coupled to capillary electrophoresis. Conductometric, amperometric, voltametric, and potentiometric modes of detection are reviewed. The positioning of the electrodes, interferences of high electric field, and the materials employed in the fabrication and modification of the electrodes
Química Nova. Publicado em: 2003-01
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10. Polyvinylpyrrolidone-Agarose Gel Electrophoresis Purification of Polymerase Chain Reaction-Amplifiable DNA from Soils
This communication describes a modification of agarose gel electrophoresis to provide a rapid and simple method for the purification of polymerase chain reaction-amplifiable DNA from soil. This modification is to add polyvinylpyrrolidone to the agarose gel. The polyvinylpyrrolidone addition retards the electrophoretic mobility of denaturing phenolic compound
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11. Modification of viral structural proteins of herpesvirus sylvilagus by glycosylation and phosphorylation.
The structural proteins of herpesvirus sylvilagus, a lymphotropic gamma herpesvirus, were analyzed by sodium dodecyl sulfate and two-dimensional polyacrylamide gel electrophoreses. Modification of the proteins by glycosylation and phosphorylation was shown by the incorporation of [14C]glucosamine or 32Pi into material which comigrated with [35S]methionine-la
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12. Modification of the Starch Block Electrophoresis Method for the Preparation of Immunoglobulin A from Multiple Myeloma Sera on a Large Scale
Separation of immunoglobulin A from a large sample of human serum from patients with multiple myeloma was done by utilizing a large zone electrophoresis apparatus.