Elaidic Acid
Mostrando 1-12 de 15 artigos, teses e dissertações.
-
1. OTIMIZAÇÃO DE METODOLOGIA DE ANÁLISE DE ÁCIDO GRAXO TRANS (C18:1 9t) POR ELETROFERESE CAPILAR EM AMOSTRAS ALIMENTÍCIAS
Foi desenvolvido um método alternativo para determinação do teor de trans total expresso em ácido elaídico utilizando eletroforese capilar de zona (CZE), com detecção indireta no UV em 224 nm e tempo de análise em torno de 8 minutos. O eletrólito de corrida otimizado é composto por 15,0 mmol L-1 KH2PO4 / Na2HPO4 (pH em torno de 6,8); Brij 35 8,0 mm
Publicado em: 2010
-
2. Saturated Fatty Acid Requirer of Neurospora crassa
Dietary saturated fatty acids containing 12- to 18-carbon atoms satisfy growth requirements of Neurospora crassa mutant cel (previously named ol; Perkins et al., reference 11); unsaturated fatty acids are synthesized by direct desaturation when an appropriate saturate is available. Odd-chain saturates, 15 carbons and 17 carbons long, satisfy the requirement,
-
3. Lipids of Treponema pallidum Kazan 5
The lipid composition of Treponema pallidum Kazan 5 cultivated in a lipid-defined medium was investigated. Lipids comprised 18 to 20% of the dry weight of the treponeme. Glycolipid and phospholipids accounted for 90 to 95% of the total lipids and free fatty acids made up the remaining 5 to 10%. The major polar lipids were the glycolipid, 1-(O-α-d-galactopyr
-
4. Relationship between bactericidal action of complement and fluidity of cellular membranes.
The mode of complement-mediated killing of Escherichia coli B cells grown at 25 and 42 degrees C and of E. coli K-12 UFAts cells grown in the presence of oleic and elaidic aicds was examined in relation to their membrane lipid compositions and the thermodynamic properties of membrane phospholipids. Phospholipids isolated from 25 degrees C-grown cells of stra
-
5. Turnover of Phospholipids in an Unsaturated Fatty Acid Auxotroph of Escherichia coli
The membrane phospholipids of an unsaturated fatty acid auxotroph of Escherichia coli were found to undergo turnover. These phospholipids were excreted into the culture medium, and were replaced in the cell with newly synthesized phospholipids. Phospholipids of growing cells supplemented with elaidic acid underwent rapid turnover, while those of cells supple
-
6. Replacement of the aliphatic chains of Clostridium acetobutylicum by exogenous fatty acids: regulation of phospholipid and glycolipid composition.
The membrane lipid aliphatic chains of Clostridium acetobutylicum ATCC 4259 have been extensively modified by growth in biotin-free medium containing vitamin-free casein hydrolysate supplemented with either elaidic acid, oleic acid, or mixtures of palmitic and oleic acids. Growth with elaidic acid resulted in polar lipids containing 88.6% 18:1 acyl chains an
-
7. Membrane-bound thioesterase activity in mycoplasmas.
Thioesterase activity was found in all mycoplasmas tested. Activity was highest in Acholeplasma species, whereas most of the sterol-requiring Mycoplasma species showed little activity. The thioesterase activity of Acholoplasma laidlawii is confined to the cell membrane. The enzyme could not be released from the membrane by either low- or high-ionic-strength
-
8. Effects of fatty acids on motility retention by Treponema pallidum in vitro.
Treponema pallidum (Nichols virulent strain) was incubated under 75% N2 + 20% H2 + 5% CO2 in prereduced serum-free modified Eagle-Richter medium supplemented with different concentrations of various long-chain fatty acids complexed with fatty acid-free bovine serum albumin. Motility retention was greater in medium with oleic acid containing 15 rather than 2
-
9. Direct modulation of secretory chloride channels by arachidonic and other cis unsaturated fatty acids.
The effect of fatty acids on Cl- channels and transepithelial Cl- secretion is investigated. Patch-clamp experiments show that arachidonic acid blocks Cl- channels in a dose-dependent manner. Kinetic analysis shows that the mean open time is decreased 10-fold with 25 microM arachidonic acid. There is a linear relationship between the reciprocal of mean open
-
10. Effect of Cerulenin on Growth and Lipid Metabolism of Mycoplasmas
Cerulenin markedly inhibited the growth of Acholeplasma laidlawii. A. axanthum and A. granularum were less susceptible, whereas the sterol-requiring Mycoplasma species examined showed very little susceptibility. The inhibition was not reversed by the addition of long-chain fatty acids to the medium. At a concentration of 20 μg/ml, cerulenin inhibited the in
-
11. Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer.
Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate incorporated into the membranes. The fatty
-
12. Effects of solvents and alcohols on the polar lipid composition of Clostridium butyricum under conditions of controlled lipid chain composition.
Clostridium butyricum has been grown in media devoid of biotin, to which long-chain fatty acids have been added to promote growth. We have shown previously that, under these conditions, exogenous fatty acids are extensively incorporated into the cellular phospholipids. Cells grown with elaidic acid, trans-9-18:1, have normal ratios of the glycerol acetal of