Egfp
Mostrando 1-12 de 242 artigos, teses e dissertações.
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1. Synthetic and minimalist vectors for Agrobacterium tumefaciens-mediated transformation of fungi
Abstract We present a collection of minimalist binary vectors for transformation through ATMT applicable to several fungi species. pLUO plasmid binary vectors consist of a reporter module containing fluorescent proteins, mCherry or eGFP, flanked by a multiple cloning site and a transcription terminator site. They also present a synthetic gene allowing resist
Genet. Mol. Biol.. Publicado em: 13/06/2019
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2. Preparation and characterization of non-viral gene delivery systems with pEGFP-C1 Plasmid DNA
ABSTRACT In recent years, non-viral delivery systems for plasmid DNA have become particularly important. They can overcome the disadvantages of viral systems such as insertional mutagenesis and unpredicted immunogenicity. Some additional advantages of non-viral gene delivery systems are; good stability, low cost, targetability, delivery of a high amount of g
Braz. J. Pharm. Sci.. Publicado em: 07/06/2018
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3. Isolation and characterization of a promoter responsive to salt, osmotic and dehydration stresses in soybean
Abstract Drought stress is the main limiting factor of soybean yield. Currently, genetic engineering has been one important tool in the development of drought-tolerant cultivars. A widely used strategy is the fusion of genes that confer tolerance under the control of the CaMV35S constitutive promoter; however, stress-responsive promoters would constitute the
Genet. Mol. Biol.. Publicado em: 27/03/2017
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4. Achievement of constitutive fluorescent pLEXSY-egfp Leishmania braziliensis and its application as an alternative method for drug screening in vitro
BACKGROUND Gene reporter-fluorescent cells have emerged as alternative method for drug screening. OBJECTIVE Achievement of constitutive expression of fluorescent protein GFP by Leishmania braziliensis as alternative method for drug screening. METHODS L. braziliensis-GFP was generated using Leishmania tarentolae pLEXSY-egfp for constitutive expression of
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02
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5. Six1 induces protein synthesis signaling expression in duck myoblasts mainly via up-regulation of mTOR
Abstract As a critical transcription factor, Six1 plays an important role in the regulation of myogenesis and muscle development. However, little is known about its regulatory mechanism associated with muscular protein synthesis. The objective of this study was to investigate the effects of overexpression ofSix1 on the expression of key protein metabolism-re
Genet. Mol. Biol.. Publicado em: 2016-03
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6. Implantation of muscle satellite cells overexpressing myogenin improves denervated muscle atrophy in rats
This study evaluated the effect of muscle satellite cells (MSCs) overexpressing myogenin (MyoG) on denervated muscle atrophy. Rat MSCs were isolated and transfected with the MyoG-EGFP plasmid vector GV143. MyoG-transfected MSCs (MTMs) were transplanted into rat gastrocnemius muscles at 1 week after surgical denervation. Controls included injections of untran
Braz J Med Biol Res. Publicado em: 05/02/2016
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7. Effects of Human Umbilical Cord Mesenchymal Stem Cells on Renal Ischaemia-reperfusion Injury in Rats
Objective This study aims to observe the function of umbilical cord-mesenchymal stem cells (UC-MSCs) labelled with enhanced green fluorescent protein (eGFP) in the repair of renal ischaemia-reperfusion (I/R) injury, to determine the effects on inflammatory cascade in an established rat model and to explore possible pathogenesis. Materials and Methods Si
Int. braz j urol.. Publicado em: 2014-08
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8. VP22 herpes simplex virus protein can transduce proteins into stem cells
The type I herpes simplex virus VP22 tegument protein is abundant and well known for its ability to translocate proteins from one cell to the other. In spite of some reports questioning its ability to translocate proteins by attributing the results observed to fixation artifacts or simple attachment to the cell membrane, VP22 has been used to deliver several
Braz J Med Biol Res. Publicado em: 01/02/2013
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9. Potencial terapêutico das células mononucleares da medula óssea em um modelo experimental de esclerose lateral amiotrófica
A esclerose lateral amiotrófica (ELA) é uma doença neurodegenerativa caracterizada por uma perda seletiva de neurônios motores na medula espinhal, tronco encefálico e córtex motor. A perda de motoneurônios dá início a uma paralisia progressiva, levando o paciente a óbito em 2 a 5 anos. O tratamento se baseia principalmente em medidas sintomáticas
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 29/08/2012
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10. Estudo sobre o efeito do transplante de células mononucleares da medula óssea no modelo de epilepsia genética no camundongo EL
Considerando-se a elevada incidência da epilepsia (2-3% da população mundial) e o alto índice de refratariedade ao tratamento com fármacos antiepilépticos disponíveis (20-30%) torna-se fundamental que sejam estudadas e disponibilizadas novas alternativas terapêuticas. Na epilepsia, a exemplo de outros distúrbios do sistema nervoso central, há perda
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 19/03/2012
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11. Expressão de um fragmento da Miosina Va inibe o crescimento de tumores de melanoma induzidos em modelo animal / Expression of a proapoptotic myosin Va fragment inhibits melanoma tumor growth in animal model
A miosina Va é uma proteína motora envolvida no transporte e posicionamento de vesículas, organelas e mRNA. Além disso, postulou-se que a miosina-Va atua no seqüestro do fator pró-apoptótico, Bmf, no citoesqueleto de actina. Pesquisas realizadas em nosso laboratório demonstraram que um fragmento da miosina Va (MVaf), que corresponde ao sítio ligante
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 27/01/2012
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12. Construção de sistemas bacterianos para a detecção de metais pesados em amostras ambientais. / Construction of bacterial systems for the detection of heavy metals in environmental samples.
Aiming at the construction of three bacterial biosensors for metals mercury, arsenic and lead, Cupriavidus metallidurans CH34 was chosen to obtain the DNA fragments (by PCR) corresponding to operons mer, ars and pbr. The fragments were inserted upstream of the EGFP gene, in plasmid pBB-EGFP, getting three new plasmids: pGHg, pGAs and pGPb. They were cloned i
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/01/2012