Dna Probes
Mostrando 1-12 de 3739 artigos, teses e dissertações.
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1. [PROVISIONAL] Low cytomolecular diversification in the genus Stylosanthes Sw. (Papilionoideae, Leguminosae)
Abstract Stylosanthes (Papilionoideae, Leguminosae) is a predominantly neotropical genus with ~ 48 species that includes worldwide important forage species. This study presents the chromosome number and morphology of eight species of the genus Stylosanthes (S. acuminata, S. gracilis, S. grandifolia, S. guianensis, S. hippocampoides, S. pilosa, S. macrocephal
Genet. Mol. Biol.. Publicado em: 19/08/2019
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2. Microbial Profile of Supragingival and Subgingival Plaque of Patients With Glycogen Storage Disease
Abstract Patients with glycogen storage disease (GSD) are either orally fed (ORF) or gastronomy-tube fed (GTF) with cornstarch to maintain normal glucose levels. It is not known whether the use of cornstarch affects the microbiological oral profile of patients with GSD. Thus, the purpose of this study was to compare supragingival and subgingival plaque sampl
J. inborn errors metab. screen.. Publicado em: 30/05/2019
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3. Validation of chromogenic in situ hybridization reactions for DNA and RNA detection in formalin-fixed paraffin-embedded tissue
RESUMO Introdução: A hibridização in situ cromogênica (CISH) é uma alternativa aos métodos tradicionais imuno-histoquímicospara diagnóstico de doenças infecciosas em amostras fixadas em formalina e incluídas em parafina, visto que apresenta grande sensibilidade e especificidade. Esse tipo de amostra sofre diversas modificações químicas durante
J. Bras. Patol. Med. Lab.. Publicado em: 09/05/2019
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4. Comparative Cytogenetics Among Three Sympatric Hypostomus Species (Siluriformes: Loricariidae): An Evolutionary Analysis in a High Endemic Region
ABSTRACT The karyotypes of three armored catfish species (Loricariidae) from the Iguaçu river, southern of the Brazil, were compared using different techniques: C-banding, Ag-NOR and fluorescence in situ hybridization (FISH), which used 5S and 18S rDNAs and total Cot-1 fraction as probes. Hypostomus commersoni and Hypostomus derbyi presented 2n = 68 chromos
Braz. arch. biol. technol.. Publicado em: 14/11/2018
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5. Performance of probe polymerization-conjunction-agarose gel electrophoresis in the rapid detection of KRAS gene mutation
Abstract This study aimed to develop a simple and rapid method to detect KRAS gene mutations for conventional clinical applications under laboratory conditions. The genotype of mutation sites was determined based on the occurrence of target bands in the corresponding lanes of the reaction tubes through polymerization-conjunction of the probes, probe purifica
Genet. Mol. Biol.. Publicado em: 16/07/2018
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6. Karyotype and genome size comparative analyses among six species of the oilseed-bearing genus Jatropha (Euphorbiaceae)
Abstract Jatropha is an important genus of Euphorbiaceae, with species largely used for various purposes, including the manufacturing of soaps and pharmaceutical products and applications in the bioenergetic industry. Although there have been several studies focusing J. curcas in various aspects, the karyotype features of Jatropha species are poorly known. T
Genet. Mol. Biol.. Publicado em: 14/05/2018
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7. Comparative cytogenetic analysis in Erythrolamprus snakes (Serpentes: Dipsadidae) from Argentina
ABSTRACT We described the karyotypes of five snake taxa from Argentina: Erythrolamprus almadensis, E. ceii, E. poecilogyrus caesius, E. p. schotti and E. p. sublineatus, and also intergrading individuals between the last two subspecies by conventional staining, chromosome bandings and fluorescent in situ hybridization (FISH) with 28S ribosomal DNA probes. Er
An. Acad. Bras. Ciênc.. Publicado em: 2018-04
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8. Association of caspase-1 polymorphisms with Chagas cardiomyopathy among individuals in Santa Cruz, Bolivia
Abstract INTRODUCTION: Trypanosoma cruzi (Tc) infection is usually acquired in childhood in endemic areas, leading to Chagas disease, which progresses to Chagas cardiomyopathy in 20-30% of infected individuals over decades. The pathogenesis of Chagas cardiomyopathy involves the host inflammatory response to T. cruzi, in which upstream caspase-1 activation p
Rev. Soc. Bras. Med. Trop.. Publicado em: 2017-08
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9. Detection and molecular characterisation of a diagnosis escape variant associated with occult hepatitis B virus in Brazil
BACKGROUND Many studies have identified mutations in the hepatitis B surface antigen (HBsAg) as important factors limiting the ability of commercial serological assays to detect this viral antigen. However, an association between mutations in the HBsAg gene and the occurrence of occult HBV infection (OBI) in patients has not been established. OBJECTIVES
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-07
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10. Standardization and evaluation of a duplex real-time quantitative PCR for the detection of Leishmania infantum DNA: a sample quality control approach
Abstract INTRODUCTION: Molecular techniques have been shown to be alternative methods for the accurate detection of infectious and parasitic diseases, such as the leishmaniases. The present study describes the optimization and evaluation of a duplex real-time quantitative PCR (qPCR) protocol developed for the simultaneous detection of Leishmania infantum DN
Rev. Soc. Bras. Med. Trop.. Publicado em: 2017-06
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11. Evaluation of the association between the TAS1R2 and TAS1R3 variants and food intake and nutritional status in children
Abstract Taste perception plays a key role in determining individual food preferences and dietary habits and may influence nutritional status. This study aimed to investigate the association of TAS1R2 (Ile191Val - rs35874116) and TAS1R3 (-1266 C/T - rs35744813) variants with food intake and nutritional status in children followed from birth until 7.7 years o
Genet. Mol. Biol.. Publicado em: 11/05/2017
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12. Use of fluorescent oligonucleotide probes for differentiation between Paracoccidioides brasiliensis and Paracoccidioides lutzii in yeast and mycelial phase
BACKGROUND Fluorescence in situ hybridisation (FISH) associated with Tyramide Signal Amplification (TSA) using oligonucleotides labeled with non-radioactive fluorophores is a promising technique for detection and differentiation of fungal species in environmental or clinical samples, being suitable for microorganisms which are difficult or even impossible
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-02