Direct Immunofluorescence
Mostrando 13-24 de 515 artigos, teses e dissertações.
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13. Sexual transmission of American trypanosomiasis in humans: a new potential pandemic route for Chagas parasites
BACKGROUND The Trypanosoma cruzi infection endemic in Latin America has now spread to several countries across four continents; this endemic involves triatomine vector-free protists. We hypothesised that the sexual transmission of T. cruzi contributes to the ongoing spread of Chagas disease. OBJECTIVES A short-term longitudinal study was conducted to eva
Mem. Inst. Oswaldo Cruz. Publicado em: 2017-06
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14. The importance of direct immunofluorescence in pemphigus herpetiformis diagnosis
Abstract Pemphigus herpetiformis is an autoimmune bullous disease, that combines clinical features of dermatitis herpetiformis and linear IgA bullous dermatosis and immunological characteristics of pemphigus, which makes this disease peculiar and this diagnosis rarely suspected in the first evaluation of the patient. The reported case is of a patient with cl
An. Bras. Dermatol.. Publicado em: 2017
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15. Prevalence of rhinovirus in wheezing children: a comparison with respiratory syncytial virus wheezing
Abstract Objective To explore the distribution and clinical manifestations of rhinovirus infection in wheezing children, and compare the clinical differences between rhinovirus- and respiratory syncytial virus-induced wheezing. Materials and methods This prospective cohort study was carried out in Children's Hospital of Soochow University from Dec 2012 to
Braz J Infect Dis. Publicado em: 2016-04
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16. Sensitivity and specificity of parallel or serial serological testing for detection of canine Leishmania infection
In Brazil, human and canine visceral leishmaniasis (CVL) caused byLeishmania infantum has undergone urbanisation since 1980, constituting a public health problem, and serological tests are tools of choice for identifying infected dogs. Until recently, the Brazilian zoonoses control program recommended enzyme-linked immunosorbent assays (ELISA) and indirect i
Mem. Inst. Oswaldo Cruz. Publicado em: 19/02/2016
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17. Performance of direct immunofluorescence assay for the detection of human metapneumovirus under clinical laboratory settings
ABSTRACT INTRODUCTION: Human metapneumovirus (hMPV) is an emergent human respiratory pathogen. This study aimed to evaluate the performance of direct immunofluorescence (DIF) to detect hMPV in a clinical laboratory setting. METHODS: Nasopharyngeal aspirate samples (448) of children and adults with respiratory illness were used to detect hMPV by using DIF
Rev. Soc. Bras. Med. Trop.. Publicado em: 2015-12
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18. Diagnostic methods to cutaneous leishmaniasis detection in domestic dogs and cats
Abstract: Cutaneous leishmaniasis is caused by different species of Leishmania. In domestic animals such as dogs and cats, the diagnostic consists of clinical, epidemiological and serological tests, which changes among countries all around the world. Because of this diversity in the methods selected, we propose this systematic literature review to identify t
An. Bras. Dermatol.. Publicado em: 2015-12
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19. Evaluation of the reference value for the Montenegro skin test
Abstract:INTRODUCTION:The Montenegro skin test (MST) has good clinical applicability and low cost for the diagnosis of American tegumentary leishmaniasis (ATL). However, no studies have validated the reference value (5mm) typically used to discriminate positive and negative results. We investigated MST results and evaluated its performance using different cu
Rev. Soc. Bras. Med. Trop.. Publicado em: 2015-08
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20. Unusual remodeling of the hyalinization band in vulval lichen sclerosus by type V collagen and ECM 1 protein
OBJECTIVES: The vulva is the primary site affected in lichen sclerosus, a chronic dermatosis in women that is histologically characterized by a zone of collagen remodeling in the superior dermis. The normal physiological properties of the vulva depend on the assembly of collagen types I (COLI), III (COLIII) and V (COLV), which form heterotypic fibers, and e
Clinics. Publicado em: 2015-05
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21. Using a top predator as a sentinel for environmental contamination with pathogenic bacteria: the Iberian wolf and leptospires
The Iberian wolf (Canis lupus) is the top predator in the Iberian environments in which it lives, feeding on a wide range of species, thus encountering a wide range of disease agents. Therefore, the wolf can serve as sentinel of environmental contamination with pathogens. We investigated the
Mem. Inst. Oswaldo Cruz. Publicado em: 09/12/2014
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22. Evaluation of parasitological examination, kDNA polymerase chain reaction and rK39-based immunochromatography for the diagnosis of visceral leishmaniasis in seropositive dogs from the screening-culling program in Brazil
Introduction Dogs play a primary role in the zoonotic cycle of visceral leishmaniasis (VL). Therefore, the accurate diagnosis of infected dogs, primarily asymptomatic dogs, is crucial to the efficiency of VL control programs. Methods We investigated the agreement of four diagnostic tests for canine visceral leishmaniasis (CVL): parasite detection, either
Rev. Soc. Bras. Med. Trop.. Publicado em: 2014-08
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23. Modeling pulmonary fibrosis by abnormal expression of telomerase/apoptosis/collagen V in experimental usual interstitial pneumonia
Limitations on tissue proliferation capacity determined by telomerase/apoptosis balance have been implicated in pathogenesis of idiopathic pulmonary fibrosis. In addition, collagen V shows promise as an inductor of apoptosis. We evaluated the quantitative relationship between the telomerase/apoptosis index, collagen V synthesis, and epithelial/fibroblast rep
Braz J Med Biol Res. Publicado em: 04/06/2014
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24. Medium-term cryopreservation of rabies virus samples
Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of r
Rev. Soc. Bras. Med. Trop.. Publicado em: 2013-12