Medium-term cryopreservation of rabies virus samples
Aguiar, Tereza D'avila de Freitas, Teixeira, Maria Fatima da Silva, Costa, Edmara Chaves, Vitaliano, Allan Bezerra, Teles, Carlos Henrique de Andrade, Barroso, Igor Ciriaco, Dias, Ronaldo Pereira, Moraes, Nelio Batista de
Rev. Soc. Bras. Med. Trop.
DATA DE PUBLICAÇÃO
Introduction The cryopreservation of rabies virus has been described in detail in the literature. To date, little information is available on the use of cryoprotective agents for cold preservation of this virus, and the available data focus only on short-term virus preservation. In this study, we investigated the medium-term cryopreservation of samples of rabies virus using different cryopreservation protocols. Methods The cryopreservation protocols for the rabies virus samples were performed at -20°C and were divided according to the variables of time and cryoprotectant type used. The laboratory tests (intracerebral inoculation of mice, viral titration and direct immunofluorescence) were performed at regular intervals (360 and 720 days) to assess the viability of the viral samples according to the different preservation techniques used. Results After 1 year of cryopreservation, the fluorescence intensity of intracellular corpuscles of the rabies virus and the median survival time of the mice differed between the positive controls and the treatments with the cryoprotectants. After 2 years, most of the samples subjected to the cryopreservation protocols (including the controls) did not produce fluorescence. However, the virus samples exposed to the cryoprotectant sucrose (68% solution) responded positively in the direct immunofluorescence assay and in the intracerebral inoculation of the mice. Conclusions Medium-term cryopreservation of the rabies virus inactivates the viral sample. However, the cryoprotectant agent sucrose (68%) produces a preservative effect in cryopreserved rabies virus samples.
- Results of Medium-Term Survival of the Non-Cemented Logical Femoral Stem
- Comparing stochastic optimization methods to solve the medium-term operation planning problem
- High Medium-term Survival of Zweymüller SLR-Plus® Stem Used in Femoral Revision
- Short and Medium-term Outcomes of Omphalocele and Gastroschisis: A Survey from a Tertiary Center
- Medium-term results of Mini-arc for urinary stress incontinence in ambulatory patients under local anesthesia