Deinococcus Radiodurans
Mostrando 13-24 de 95 artigos, teses e dissertações.
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13. Targeted Mutagenesis by Duplication Insertion in the Radioresistant Bacterium Deinococcus radiodurans: Radiation Sensitivities of Catalase (katA) and Superoxide Dismutase (sodA) Mutants
Deinococcus radiodurans R1 is extremely resistant to both oxidative stress and ionizing radiation. A simple and general targeted mutagenesis method was developed to generate catalase (katA) and superoxide dismutase (sodA) mutants. Both mutants were shown to be more sensitive to ionizing radiation than the wild type.
American Society for Microbiology.
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14. Promoter Cloning in the Radioresistant Bacterium Deinococcus radiodurans
Deinococcus radiodurans is a highly radiation-resistant bacterium that is classed in a major subbranch of the bacterial domain. Since very little is known about gene expression in this bacterium, an initial study of promoters was undertaken. In order to isolate promoters and study promoter function, a series of integrative vectors for stable chromosomal inse
American Society for Microbiology.
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15. Peptidoglycan Fine Structure of the Radiotolerant Bacterium Deinococcus radiodurans Sark
Peptidoglycan from Deinococcus radiodurans was analyzed by high-performance liquid chromatography and mass spectrometry. The monomeric subunit was: N-acetylglucosamine–N-acetylmuramic acid–l-Ala–d-Glu-(γ)–l-Orn-[(δ)Gly-Gly]–d-Ala–d-Ala. Cross-linkage was mediated by (Gly)2 bridges, and glycan strands were terminated in (1→6)anhydro-muramic ac
American Society for Microbiology.
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16. Protein Splicing of the Deinococcus radiodurans Strain R1 Snf2 Intein
Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein that splices by the Ala1 protein splicing p
American Society for Microbiology.
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17. Engineering Deinococcus geothermalis for Bioremediation of High-Temperature Radioactive Waste Environments
Deinococcus geothermalis is an extremely radiation-resistant thermophilic bacterium closely related to the mesophile Deinococcus radiodurans, which is being engineered for in situ bioremediation of radioactive wastes. We report that D. geothermalis is transformable with plasmids designed for D. radiodurans and have generated a Hg(II)-resistant D. geothermali
American Society for Microbiology.
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18. Ro ribonucleoproteins contribute to the resistance of Deinococcus radiodurans to ultraviolet irradiation
The genome of the radiation-resistant eubacterium Deinococcus radiodurans contains an ortholog of an RNA-binding protein known as the Ro 60-kD autoantigen. This protein, which was previously identified only in higher eukaryotes, is normally bound to small RNAs known as Y RNAs. We show that the Ro protein ortholog Rsr contributes to the resistance of D. radio
Cold Spring Harbor Laboratory Press.
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19. Expression of recA in Deinococcus radiodurans.
Deinococcus (formerly Micrococcus) radiodurans is remarkable for its extraordinary resistance to ionizing and UV irradiation and many other agents that damage DNA. This organism can repair > 100 double-strand breaks per chromosome induced by ionizing radiation without lethality or mutagenesis. We have previously observed that expression of D. radiodurans rec
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20. Structure of Deinococcus radiodurans tunicamycin-resistance protein (TmrD), a phosphotransferase
The 1.95 Å resolution X-ray crystal structure of tunicamycin-resistance protein (TmrD) from D. radiodurans is described.
International Union of Crystallography.
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21. Identification and characterization of uvrA, a DNA repair gene of Deinococcus radiodurans.
Deinococcus radiodurans is extraordinarily resistant to DNA damage, because of its unusually efficient DNA repair processes. The mtcA+ and mtcB+ genes of D. radiodurans, both implicated in excision repair, have been cloned and sequenced, showing that they are a single gene, highly homologous to the uvrA+ genes of other bacteria. The Escherichia coli uvrA+ ge
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22. Gene fusions with lacZ by duplication insertion in the radioresistant bacterium Deinococcus radiodurans.
Deinococcus radiodurans is the most-studied species of a eubacterial family characterized by extreme resistance to DNA damage. We have focused on developing molecular biological techniques to investigate the genetics of this organism. We report construction of lacZ gene fusions by a method involving both in vitro splicing and the natural transformation of D.
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23. Interchromosomal recombination in the extremely radioresistant bacterium Deinococcus radiodurans.
Deinococcus radiodurans and other members of the genus Deinococcus are remarkable for their extreme resistance to ionizing radiation and many other agents that damage DNA. We have recently shown that recombinational processes participate in interplasmidic repair following in vivo irradiation. We now present direct studies on interchromosomal recombination am
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24. Repair of Oxidized Bases in the Extremely Radiation-Resistant Bacterium Deinococcus radiodurans
Deinococcus radiodurans is able to resist and survive extreme DNA damage induced by ionizing radiation and many other DNA-damaging agents. It is believed that it possesses highly efficient DNA repair mechanisms. To characterize the repair pathway of oxidized purines in this bacteria, we have purified, from crude extracts, proteins that recognize these oxidiz
American Society for Microbiology.