Cysteine Protease
Mostrando 25-36 de 426 artigos, teses e dissertações.
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25. Structural and functional studies about Xylellain, the cysteine protease from bacterium / Estudos estruturais e funcionais da Xylellaína, uma cisteíno protease da bactéria Xylella fastidiosa
A Xylella fastidiosa é uma bactéria gram-negativa que infecta o xilema das plantas causando muitas vezes a maturação precoce e a diminuição dos frutos. Ela é responsável por importantes perdas na economia, no Brasil é a causadora de doenças de Citrus Variegated Chlorosis (CVC) e a da doença de Pierce nos Estados Unidos. As proteases desempenham fu
Publicado em: 2008
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26. Filogeografia molecular e atividade de enzimas digestivas do caruncho-do-feijão Acanthoscelides obtectus (Bruchidae:Coleoptera) / Phylogeography and molecular activity of digestive enzymes of bean-weevil, Acanthoscelides obtectus (Bruchidae:Coleoptera)
In the world, Brazil stands out as a major producer and consumer in the world of common bean, Phaseolus vulgaris, however has low productivity due to several factors among them, low technological level used in the cultivation and lack of care mainly plant grain during the storage of . Large losses in the storage of bean (P. vulgaris) are attributed to grain
Publicado em: 2008
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27. CaracterizaÃÃo fÃsico-quÃmica e purificaÃÃo da bromelina do Ananas comosus (L.) Merril (Abacaxi-Bromeliaceae)
Bromelina à o nome genÃrico dado ao grupo de enzimas proteolÃticas encontrada nos vegetais da famÃlia Bromeliaceae, da qual o abacaxi à o mais conhecido e rico nesta protease, que tem grande importÃncia na Ãrea de saÃde e de alimentos. O objetivo deste trabalho foi determinar as propriedades fÃsico-quÃmicas e purificar a bromelina presente no Anana
Publicado em: 2008
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28. Produção recombinante e estudos funcionais de três novas cistatinas da cana-de-açúcar e sua utilização em estudos de inibição da adesão, proliferação, migração e invasão celular
ABSTRACT Tumor metastasis is usually a major cause of death among cancer patients. Degradation and invasion of the extracellular matrix and basement membrane, key steps in the metastatic process, rely on the activity of proteolytic enzymes. The levels and activities of cathepsins B and L are considerably increased in cancer cells, and have been related to th
Publicado em: 2008
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29. Identificação e caracterização de proteinas expressas no espaço intercelular de folhas de Theobroma cacao na interação com Moniliophthora perniciosa / Identification and characterization of proteins expressed in the intercellular space of leves from Theobroma cacao infected by Moniliophthora perniciosa
The invasion of the plant apoplast is a critical phase of the infection for numerous pathogens, including the fungus M. perniciosa (Mp) that remains in the intercellular space at the parasitic stage until about 60 days. Thus, the understanding of plant defenses induced in the apoplasm and the response mechanisms that the pathogens used for bypassing these de
Publicado em: 2008
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30. Estudos funcionais de uma possível cisteíno protease de Xanthomonas axonopodis pv. citri.
Cysteine proteases are enzymes found in many organisms and are characterized by the presence of cysteine residue in their active site. These proteases play an important role in the organism metabolism. In pathogenic organisms, for example, cysteine proteases may be involved in the pathogenicity process by unbalancing of the biological functions of the host (
Publicado em: 2007
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31. Expressão recombinante da canacistatina em células de inseto.
Due to the great economic importance of sugarcane crop in Brazil, the occurrence of infections by phytopathogens leading to decreased productivity and quality remains a serious problem. As the plants have natural mechanisms of defense against the attack of fungi and insects, amongst which the inhibitors of proteases are distinguished, the use of these substa
Publicado em: 2007
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32. Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro
Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultur
Memórias do Instituto Oswaldo Cruz. Publicado em: 2006-09
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33. Isolamento e caracterização de um novo conjunto de serinoproteases com atividade trombina-like e de L-aminoacido oxidase do veneno de Crotalus durissus cascavella / Isolation and characterization of a new serine proteases group with thrombinin-like and L-amino acid oxidase activity from Crotalus durissus cascavella
The use of the isolated toxins from poisons as molecular tools in the understanding of diverse physiological and pathological events has been proved by some works in literature. The serpent Crotalus durissus cascavella is found in the areas of Caatinga Northeast of Brazil, since Maranhão until North of the state of Minas Gerais, and in spite of it hasn?t be
Publicado em: 2006
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34. Bmcystatin, cysteine protease inhibitor present in fat body of Boophilus microplus tick: Cloning, expression, purification and characterization. / BmCistatina, Inibidor de cisteinoproteases presente em corpo gorduroso de carrapato Boophilus microplus: Clonagem, expressão, purificação e caracterização.
A importância econômica dos carrapatos está relacionada a seus hábitos hematofágicos. Entre as espécies que pertencem à família Ixodidae, o carrapato Boophilus microplus tem grande importância veterinária por ser vetor de patógenos, atuando como ectoparasito em animais domésticos e silvestres, transmitindo a doença conhecida como o babesiosis e
Publicado em: 2006
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35. Study of physiochemical properties and criteria for obtaining and validation of QSAR models of similar semicarbazonas set with antichagasic activity, selected of literature / Estudo de propriedades físico-químicas e de critérios para obtenção e validação de modelos QSAR para séries de análogos de semicarbazonas com atividade antichagásica, retiradas da literatura
It has been observed an enormous improvement in the methods concerning data generation, leading to a large amount of information, especially for chemical and biological systems. Through these developments, it becomes relevant to have reliable methods, mainly new mathematical tools, for structure-activity relationship (SAR) data examination, which means that
Publicado em: 2005
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36. Caracterização funcional e estrutural de uma metaloprotease hemorrágica isolada da peçonha de Bothrops jararacussu. / Functional and structural characterization of a hemorrhagic metalloprotease isolated from Bothrops jararacussu snake venom
In this study the isolation, functional and structural characterization of a hemorrhagic metalloprotease, named BjussuMP-I is reported. The protein was isolated from Bothrops jararacussu snake venom by a combination of two chromatographic steps, using gel filtration on Sephacryl S-200 (0.01 M Tris-HCl, pH7.6 buffer) and Phenyl-Sepharose CL-4B chromatography
Publicado em: 2005