Giardia duodenalis: analysis of secreted proteases upon trophozoite-epithelial cell interaction in vitro

AUTOR(ES)

Rodríguez-Fuentes, Guadalupe Beatriz, Cedillo-Rivera, Roberto, Fonseca-Liñán, Rocío, Argüello-García, Raúl, Muñoz, Onofre, Ortega-Pierres, Guadalupe, Yépez-Mulia, Lilián

FONTE

Memórias do Instituto Oswaldo Cruz

DATA DE PUBLICAÇÃO

2006-09

RESUMO

Protease secretion by Giardia duodenalis trophozoites upon interaction with epithelial cells and its association with the parasite adhesion was studied in co-cultures of parasites with IEC6 epithelial cell monolayers in the presence or absence of protease inhibitors. Proteolytic activity in supernatants from trophozoites was enhanced when they were co-cultured with IEC6 cells. This activity was strongly inhibited by pre-incubation of live trophozoites with E-64 and TPCK and a concomitant inhibition of parasite adhesion to IEC6 cells was observed. These data suggest that trophozoites secrete cysteine-type proteases that play a role in the adhesion of G. duodenalis to epithelial cells.

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