Consensus Motif
Mostrando 1-12 de 671 artigos, teses e dissertações.
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1. Variability of the conserved V3 loop tip motif in HIV-1 subtype B isolates collected from Brazilian and French patients
The diversity of the V3 loop tip motif sequences of HIV-1 subtype B was analyzed in patients from Botucatu (Brazil) and Montpellier (France). Overall, 37 tetrameric tip motifs were identified, 28 and 17 of them being recognized in Brazilian and French patients, respectively. The GPGR (P) motif was predominant in French but not in Brazilian patients (53.5% vs
Brazilian Journal of Microbiology. Publicado em: 2010-10
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2. Purification and binding analysis of the nitrogen fixation regulatory NifA protein from Azospirillum brasilense
NifA protein activates transcription of nitrogen fixation operons by the alternative s54 holoenzyme form of RNA polymerase. This protein binds to a well-defined upstream activator sequence (UAS) located at the -200/-100 position of nif promoters with the consensus motif TGTN10- ACA. NifA of Azospirillum brasilense was purified in the form of a glutathione-S-
Publicado em: 2010
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3. Determinação de motivos de ligação à quitina em vicilinas de Canavalia ensiformis e Vigna unguiculata através de métodos in silico e relação com suas toxicidades para o bruquídeo Callosobruchus maculatus (Coleoptera:Bruchidae)
Chitin is an important structural component of the cellular wall of fungi and exoskeleton of many invertebrate plagues, such as insects and nematodes. In digestory systems of insects it forms a named matrix of peritrophic membrane. One of the most studied interaction models protein-carbohydrate is the model that involves chitin-binding proteins. Among the in
Publicado em: 2009
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4. Seleção e caracterização de peptídeos recombinantes ligantes a anticorpos monoclonais reativos a proteínas de Anaplasma marginale
Bovine anaplasmosis is caused by Anaplasma marginale and A. centrale. The most pathogenic and important species for cattle production is A. marginale, and is widely distributed in tropical, subtropical and temperate regions of the world. A. marginale is an intra-erythrocyte rickettsia of susceptible ruminants, biological and mechanically transmitted by ticks
Publicado em: 2008
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5. Identificação e caracterização de elementos de transposição no genoma de Rhynchosciara / Identification e characterization of transposable elements in genome of Rhynchosciara
Transposable elements are discrete sequences that are able to move from one locus to another within the genome, constituting a significant part of eukaryotic genome. They are grouped into two main types, Class I elements transpose via an RNA intermediate (retrotransposon), and Class II elements transpose via a DNA "cut-and-paste" mechanism (transposons). The
Publicado em: 2007
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6. HeurÃstica de RegulaÃÃo CombinatÃria na ReconstruÃÃo de Redes de Genes / HeurÃstica de RegulaÃÃo CombinatÃria na ReconstruÃÃo de Redes de Genes
Um dos principais objetivos da biologia molecular à descobrir o funcionamento de redes complexas de interaÃÃo entre elementos celulares. Nas Ãltimas dÃcadas um grande volume de dados biolÃgicos vem sendo produzido assim como modelos computacionais que fazem uso destes dados. Os mÃtodos computacionais para ReconstruÃÃo de Redes de Genes apresentam-se
Publicado em: 2006
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7. Seleção e caracterização de peptídeos recombinantes miméticos de antígenos do vírus da dengue por "PHAGE DISPLAY"
Dengue fever is caused by an arbovirus of the Flaviridae family, transmitted from person to another through an intermediate fly vector, Aedes aegypti. It is a tropical and subtropical infectious disease characterized by fever and strong pain in joints, which could also lead to bleeding in its hemorrhagic form. In this investigation, Phage Display technology
Publicado em: 2006
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8. Identificação e caracterização de uma proteína com motivos zinc finger de Trypanosoma cruzi
Zinc fingers are compact protein domains composed of a α-helix and a β-sheet held together by a zinc ion. Tandem arrays of zinc fingers are commonly used to recognize nucleic acids. Among other activities, they are involved in the processes of replication, transcription, and DNA repair. The nucleocapsid protein of HIV-1 contains a zinc finger motif
Publicado em: 2006
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9. AnÃlise in silico de uma matriz DRE na seqÃÃncia promotora de genes da Levedura Saccharomyces cerevisiae
We report an in silico analysis to identify a nucleotide sequence motif in DNA repair genes that may define a binding site for regulatory proteins during the induction of those genes by the presence of mutagens. The DRE-matrix weight matrix generated in this analysis was used to search for homologous sequences in the promoter region of all genes (including p
Publicado em: 2004
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10. Isolamento de análogos de genes de resistência (RGAs) em arachis cardernasii
Wild species of Arachis have been characterized by their high level of genetic polymorphism. Included among these species is Arachis cardenasii that is well known for showing resistances to different diseases. The gene products that are responsable for the resistance to various species of plants have conserved domains, and these domains can be used as molecu
Publicado em: 2002
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11. An IL-2 response element in the human IL-2 receptor alpha chain promoter is a composite element that binds Stat5, Elf-1, HMG-I(Y) and a GATA family protein.
Expression of the human interleukin-2 (IL-2) receptor alpha chain gene is potently upregulated by its own ligand, IL-2. In this study, we characterize an essential upstream IL-2 response element that contains both consensus and non-consensus GAS motifs, two putative Ets binding sites (EBS), one of which overlaps the consensus GAS motif, and a GATA motif, whi
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12. A consensus motif in the RFX DNA binding domain and binding domain mutants with altered specificity.
The RFX DNA binding domain is a novel motif that has been conserved in a growing number of dimeric DNA-binding proteins, having diverse regulatory functions, in eukaryotic organisms ranging from yeasts to humans. To characterize this novel motif, we have performed a detailed dissection of the site-specific DNA binding activity of RFX1, a prototypical member