Chimeric Sequence
Mostrando 1-12 de 1113 artigos, teses e dissertações.
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1. ISOLATION AND CHARACTERIZATION OF MICROSATELLITE MARKERS FROM Aegla Longirostri (CRUSTACEA, DECAPODA, ANOMURA) / ISOLAMENTO E CARACTERIZAÇÃO DE MICROSSATÉLITES EM AEGLA LONGIROSTRI (CRUSTACEA, DECAPODA, ANOMURA)
Aeglidae é a única família de crustáceos anomuros cujos representantes vivos são habitantes exclusivos de água doce. A origem marinha do grupo é evidenciada pelos registros fósseis. Entretanto, o gênero Aegla conquistou o ambiente dulcícola na região temperada do sul da América do Sul onde é endêmico, amplamente distribuído e diversificado, co
Publicado em: 2007
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2. Caracterização da localização subcelular da proteína THI1 de Arabidopsis thaliana. / Characterization of the subcellular localization of arabidopsis thaliana thip.
Arabidopsis thaliana thi1 gene product is probably involved in both thiamine biosynthesis as well as protection of organellar DNA from damage. Studies of thiamine biosynthesis in plants suggest a plastid location for the pathway, which is in agreement with the predicted THI1 N-terminal chloroplastic transit peptide (TP). On the other hand, thiamine is synthe
Publicado em: 2002
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3. A new computational method for detection of chimeric 16S rRNA artifacts generated by PCR amplification from mixed bacterial populations.
A new computational method (chimeric alignment) has been developed to detect chimeric 16S rRNA artifacts generated during PCR amplification from mixed bacterial populations. In contrast to other nearest-neighbor methods (e.g., CHECK_CHIMERA) that define sequence similarity by k-tuple matching, the chimeric alignment method uses the score from dynamic program
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4. Transfection of beta-casein chimeric gene and hormonal induction of its expression in primary murine mammary epithelial cells.
To study the regulatory sequence elements responsible for casein gene expression, we constructed a chimeric gene containing 5.3 kilobases (kb) of the 5'-flanking sequence and 1.6 kb of the 3'-flanking sequence of the mouse beta-casein gene fused to the bacterial chloramphenicol acetyl-transferase (CAT) gene. The chimeric gene was transfected by the calcium p
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5. Analysis of picornavirus 2A(pro) proteins: separation of proteinase from translation and replication functions.
The poliovirus (PV) genome was manipulated by replacing its 2A-encoding sequence with the corresponding sequence of coxsackie B4 virus (CBV4) or human rhinovirus type 2 (HRV2). In vitro translation of the resulting chimeric PV genomes revealed a normal cis-cleavage activity for both heterologous 2A(pro) proteinases in the chimeric PV polyproteins. However, o
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6. A leader peptide is sufficient to direct mitochondrial import of a chimeric protein.
Most mitochondrial proteins are encoded in the nucleus and synthesized in the cytoplasm as larger precursors containing NH2-terminal 'leader' peptides. To test whether a leader peptide is sufficient to direct mitochondrial import, we fused the cloned nucleotide sequence encoding the leader peptide of the mitochondrial matrix enzyme ornithine transcarbamylase
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7. Construction and use of chimeric SPR/phi 3T DNA methyltransferases in the definition of sequence recognizing enzyme regions.
Multispecific DNA methyltransferases (Mtases) of temperate Bacillus subtilis phages SPR and phi 3T methylate the internal cytosine of the sequence GGCC. They differ in their capacity to methylate additional sequences. These are CCGG and CC(A/T)GG in SPR and GCNGC in phi 3T. Introducing unique restriction sites at equivalent locations within the two genes fac
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8. Coreceptor-Dependent Inhibition of the Cell Fusion Activity of Simian Immunodeficiency Virus Env Proteins
The cytoplasmic tail (R peptide) sequence is able to regulate the fusion activity of the murine leukemia virus (MuLV) envelope (Env) protein. We have previously shown that this sequence exerts a profound inhibitory effect on the fusion activity of simian immunodeficiency virus (SIV)-MuLV chimeric Env proteins which contain the extracellular and transmembrane
American Society for Microbiology.
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9. Frequency of formation of chimeric molecules as a consequence of PCR coamplification of 16S rRNA genes from mixed bacterial genomes.
PCR is routinely used in amplification and cloning of rRNA genes from environmental DNA samples for studies of microbial community structure and identification of novel organisms. There have been concerns about generation of chimeric sequences as a consequence of PCR coamplification of highly conserved genes, because such sequences may lead to reports of non
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10. Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions.
An RGD-containing epitope from the foot-and-mouth disease virus (FMDV) VP1 protein was inserted into the e1 loop of the hepatitis B virus core (HBc) protein. This chimeric protein was expressed at high levels in Escherichia coli and spontaneously assembled into virus-like particles which could be readily purified. These fusion particles elicited high levels
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11. Diversification and Alteration of Recognition Specificity of the Pollen Ligand SP11/SCR in Self-Incompatibility of Brassica and RaphanusW⃞
The recognition specificity of the pollen ligand of self-incompatibility (SP11/SCR) was investigated using Brassica rapa transgenic plants expressing SP11 transgenes, and SP11 of Raphanus sativus S-21 was found to have the same recognition specificity as that of B. rapa S-9. In a set of three S haplotypes, whose sequence identities of SP11 and SRK are fairly
American Society of Plant Biologists.
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12. Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end o
American Society for Microbiology.