Yellow Fever Virus Replicons as an Expression System for Hepatitis C Virus Structural Proteins
AUTOR(ES)
Molenkamp, Richard
FONTE
American Society for Microbiology
RESUMO
Chimeric yellow fever virus (YF) RNAs were constructed in which the YF structural genes were replaced by the hepatitis C virus (HCV) structural genes or fusions between the YF and HCV structural genes. Interestingly, RNA replication required nucleotide complementarity between the 3′-located conserved sequence 1 and an RNA sequence located in the 5′ end of the YF capsid sequence. The (chimeric-)HCV structural proteins were efficiently expressed and processed, and the native E1/E2 heterodimer was formed. However, no indication for the production of HCV-like particles was obtained.
ACESSO AO ARTIGO
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=140782Documentos Relacionados
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