Charge Coupled Device Imaging
Mostrando 1-12 de 18 artigos, teses e dissertações.
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1. Determination of ochratoxin A in wine by high-performance thin-layer chromatography using charged coupled device
Um método foi desenvolvido e validado para a determinação de ocratoxina A (OTA) em vinho. Foi utilizado um sistema contendo um detector de carga acoplada (CCD) para adquirir as imagens fluorescentes da micotoxina obtidas a partir das placas de cromatografia em camada delgada de alta eficiência (HPTLC) sob luz ultravioleta. O método desenvolvido mostrou
Publicado em: 2011
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2. Analysis of time-resolved laser plasma ablation using an imaging spectra technique
Pulsed laser deposition (PLD) is extensively employed for the growth of thin films. The laser-material interaction involves complex processes of heating, melting, vaporization, ejection of atoms, ions and molecules, shock waves, plasma initiation, expansion and deposition onto a substrate. The understanding of the spatial and temporal distribution of a plasm
Brazilian Journal of Physics. Publicado em: 2007-12
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3. Digital chemiluminescence imaging of DNA sequencing blots using a charge-coupled device camera.
Digital chemiluminescence imaging with a cryogenically cooled charge-coupled device (CCD) camera is used to visualize DNA sequencing fragments covalently bound to a blotting membrane. The detection is based on DNA hybridization with an alkaline phosphatase(AP) labeled oligodeoxyribonucleotide probe and AP triggered chemiluminescence of the substrate 3-(2'-sp
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4. Noninvasive imaging of protein–protein interactions in living subjects by using reporter protein complementation and reconstitution strategies
In this study we have developed bioluminescence-imaging strategies to noninvasively and quantitatively image protein—protein interactions in living mice by using a cooled charge-coupled device camera and split reporter technology. We validate both complementation and intein-mediated reconstitution of split firefly luciferase proteins driven by the interact
National Academy of Sciences.
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5. Measurement of marine picoplankton cell size by using a cooled, charge-coupled device camera with image-analyzed fluorescence microscopy.
Accurate measurement of the biomass and size distribution of picoplankton cells (0.2 to 2.0 microns) is paramount in characterizing their contribution to the oceanic food web and global biogeochemical cycling. Image-analyzed fluorescence microscopy, usually based on video camera technology, allows detailed measurements of individual cells to be taken. The ap
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6. High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase
Nucleocytoplasmic trafficking of functional proteins plays a key role in regulating gene expressions in response to extracellular signals. We developed a genetically encoded bioluminescent indicator for monitoring the nuclear trafficking of target proteins in vitro and in vivo. The principle is based on reconstitution of split fragments of Renilla reniformis
National Academy of Sciences.
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7. Spatial–temporal imaging of bacterial infection and antibiotic response in intact animals
We describe imaging the luminance of green fluorescent protein (GFP)-expressing bacteria from outside intact infected animals. This simple, nonintrusive technique can show in great detail the spatial–temporal behavior of the infectious process. The bacteria, expressing the GFP, are sufficiently bright as to be clearly visible from outside the infected
National Academy of Sciences.
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8. Noninvasive Bioluminescence Imaging of Herpes Simplex Virus Type 1 Infection and Therapy in Living Mice
Mouse models of herpes simplex virus type 1 (HSV-1) infection provide significant insights into viral and host genes that regulate disease pathogenesis, but conventional methods to determine the full extent of viral spread and replication typically require the sacrifice of infected animals. To develop a noninvasive method for detecting HSV-1 in living mice,
American Society for Microbiology.
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9. High-resolution visualization of airspace structures in intact mice via synchrotron phase-contrast X-ray imaging (PCXI)
Anatomical visualization of airspace-containing organs in intact small animals has been limited by the resolution and contrast available from current imaging methods such as X-ray, micro-computed tomography and magnetic resonance imaging. Determining structural relationships and detailed anatomy has therefore relied on suitable fixation, sectioning and histo
Blackwell Science Inc.
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10. Fluorescence lifetime imaging of free and protein-bound NADH.
We introduce a methodology, fluorescence lifetime imaging (FLIM), in which the contrast depends on the fluorescence lifetime at each point in a two-dimensional image and not on the local concentration and/or intensity of the fluorophore. We used FLIM to create lifetime images of NADH when free in solution and when bound to malate dehydrogenase. This represen
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11. Two-step transcriptional amplification as a method for imaging reporter gene expression using weak promoters
We are developing assays to image tissue-specific reporter gene expression in living mice by using optical methods and positron emission tomography. Approaches for imaging reporter gene expression depend on robust levels of mRNA and reporter protein. Attempts to image reporter gene expression driven by weak promoters are often hampered by the poor trans
The National Academy of Sciences.
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12. Whole-body optical imaging of green fluorescent protein-expressing tumors and metastases
We have imaged, in real time, fluorescent tumors growing and metastasizing in live mice. The whole-body optical imaging system is external and noninvasive. It affords unprecedented continuous visual monitoring of malignant growth and spread within intact animals. We have established new human and rodent tumors that stably express very high levels of the Aequ
The National Academy of Sciences.