Cell Suspension
Mostrando 13-24 de 1250 artigos, teses e dissertações.
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13. Enhanced Accumulation of Scopoletin in Cell Suspension Culture of Spilanthes acmella Murr. Using Precursor Feeding
In this study, the various concentrations of casein hydrolysate (25, 50, 75, 100 mg/L) and L-phenylalanine (50, 100, 150, 200 µM/l) were incorporated in MS containing 15 µM BA plus 5 µM 2,4-D for enhancement of secondary metabolites in cell culture of Spilanthes acmella. The presence of casein hydrolysate in the nutrient medium improved the growth of cell
Braz. arch. biol. technol.. Publicado em: 29/04/2016
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14. Cynomolgus monkeys (Macaca fascicularis) experimentally infected with B19V and hepatitis A virus: no evidence of the co-infection as a cause of acute liver failure
This study was conducted to analyse the course and the outcome of the liver disease in the co-infected animals in order to evaluate a possible synergic effect of human parvovirus B19 (B19V) and hepatitis A virus (HAV) co-infection. Nine adult cynomolgus monkeys were inoculated with serum obtained from a fatal case of B19V infection and/or a faecal suspension
Mem. Inst. Oswaldo Cruz. Publicado em: 2016-04
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15. Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus enc
Braz. arch. biol. technol.. Publicado em: 2015-12
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16. Evaluation of limonoid production in suspension cell culture of Citrus sinensis
ABSTRACTThe use of cell and plant tissue culture techniques to produce economically important active metabolites has been growing. Among these substances are total limonoid aglycones, which are produced by "pera" orange (Citrus sinensis (L.) Osbeck, Rutaceae) and have received considerable attention because of their anticancer actions. The main objective of
Rev. bras. farmacogn.. Publicado em: 2015-10
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17. Antimicrobial activity of
Anonna mucosa (Jacq.) grownin vivo and obtained byin vitro cultureBrazilian flora includes numerous species of medicinal importance that can be used to develop new drugs. Plant tissue culture offers strategies for conservation and use of these species allowing continuous production of plants and bioactive substances.
Annona mucosa has produced substances such as acetogenins and alkaloids that exhibit anBraz. J. Microbiol.. Publicado em: 2015-09
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18. Effects of ethyl pyruvate on leukocyte-endothelial interactions in the mesenteric microcirculation during early sepsis treatment
OBJECTIVES: Experimental studies on sepsis have demonstrated that ethyl pyruvate is endowed with antioxidant and anti-inflammatory properties. This study aimed to investigate the effects of ethyl pyruvate on leukocyte-endothelial interactions in the mesenteric microcirculation in a live Escherichia coli-induced sepsis model in rats. METHODS: Male Wistar rat
Clinics. Publicado em: 2015
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19. Interaction of an opportunistic fungus Purpureocillium lilacinum with human macrophages and dendritic cells
IntroductionPurpureocillium lilacinum is emerging as a causal agent of hyalohyphomycosis that is refractory to antifungal drugs; however, the pathogenic mechanisms underlying P. lilacinum infection are not understood. In this study, we investigated the interaction of P. lilacinum conidia with human macrophages and dendritic cells in vitro.MethodsSpores of a
Rev. Soc. Bras. Med. Trop.. Publicado em: 2014-10
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20. Histopathological evaluation of tumor necrosis and volume after cyanogenic chemotherapy
PURPOSE:To determine the percentage of tumoral necrosis and volume after cyanogenic chemotherapy.METHODS:Histopathological findings of 20 Swiss mice inoculated subcutaneously in the left abdominal wall with 0.05 ml of cell suspension containing 2.5 x 105 viable cells of the Ehrlich tumor were evaluated. The tumor response to cyanogenic chemotherapy was deter
Acta Cir. Bras.. Publicado em: 2014
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21. Numerical analysis of impedance spectra of yeast suspensions
Analysis of impedance spectrum of electroporated biological cell suspensions allows evaluating the state of permeabilization produced by the applied electric field. In this article we used a genetic algorithm to determine parameters of a proposed model for the impedance of suspension adjusting them numerically in order to minimize the error in relation to ex
J. Microw. Optoelectron. Electromagn. Appl.. Publicado em: 2013-12
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22. Antifungal compounds as a mechanism to control Hemileia vastatrix by antagonistic bacteria
Pseudomonas putida P286 and Bacillus thuringiensis B157 controlled coffee rust under greenhouse and field conditions. Now we studied the mechanism(s) of antagonism of both bacteria to Hemileia vastatrix. Bacterial effects on urediniospore germination and disease severity were evaluated in experiments with six treatments: (i) growth broth, (ii) supernatant ce
Trop. plant pathol.. Publicado em: 2013-10
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23. Influence of substratum position and acquired pellicle on Candida albicans biofilm
The purpose of this study was to evaluate the influence of the substratum position and the saliva acquired pellicle (AP) on Candida albicans biofilm development. Poly(methylmethacrylate) (PMMA) disks were fabricated and randomly allocated to experimental groups: HNP (disks placed in a horizontal position and uncoated by pellicle), VNP (disks placed in a vert
Braz. oral res.. Publicado em: 11/06/2013
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24. Protective effect of flavonoids against reactive oxygen species production in sickle cell anemia patients treated with hydroxyurea
OBJECTIVE: The aim of this study was to evaluate the protective effects of quercetin, rutin, hesperidin and myricetin against reactive oxygen species production with the oxidizing action of tert-butylhydroperoxide in erythrocytes from normal subjects and sickle cell anemia carriers treated with hydroxyurea. METHODS: Detection of intracellular reactive oxygen
Rev. Bras. Hematol. Hemoter.. Publicado em: 2013