Production of Lentiviral Vectors Encoding Recombinant Factor VIII Expression in Serum-Free Suspension Cultures
AUTOR(ES)
Caron, Angelo Luis, Picanço-Castro, Virgínia, Ansorge, Sven, Covas, Dimas Tadeu, Kamen, Amine, Swiech, Kamilla
FONTE
Braz. arch. biol. technol.
DATA DE PUBLICAÇÃO
2015-12
RESUMO
ABSTRACT Lentiviral vector-mediated gene transfer offers several advantages over other gene delivery vectors when considering gene and cell therapy applications. However, using these therapies in clinical applications involves large-scale vector production in an efficient and cost-effective manner. Here we describe a high yield production of a lentivirus encoding recombinant factor VIII in a scalable and GMP-compliant culture system, based on serum free suspension cultures and transient transfection with an inexpensive reagent, polyethylenimine (PEI), reaching a total viral yield of 2.48x108 particles.
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