Cell Culture Techniques
Mostrando 37-48 de 367 artigos, teses e dissertações.
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37. Induced sputum versus bronchoalveolar lavage in the diagnosis of pneumocystis jiroveci pneumonia in human immunodeficiency virus-positive patients
Induced sputum is a useful technique for assessing airway inflammation, but its role in the diagnosis of lung disease in immunosuppressed patients needs further investigation. This study compared the use of induced sputum and BAL in the diagnosis of pneumocystosis, in HIV patients. From January 1, 2001, to December 30, 2002, HIV-positive patients older than
Brazilian Journal of Infectious Diseases. Publicado em: 2007-12
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38. Análise citopatológica de células de inseto infectadas com baculovírus mutantes
Advances in cell culture techniques allowed the isolation and precise characterization of baculoviruses mutants. The majority of these mutants is derived from the virus AcMNPV, mainly due to its facility of propagation and stability in cell culture. During the purification routine of a recombinant baculovirus, two mutants derived from AcMNPV, vSynlitx1B12P (
Publicado em: 2007
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39. Estratégias para prospecção e predição de peptídeos bioativos
Chapters one and two deal with the isolation and identification of bioactive peptides from the skin secretion of Phyllomedusa hypochondrialis. The first chapter demonstrates the use of protemic techniques, mainly mass spectrometry, for the study of bradykinin related peptides (BRPs) present in the amphibians secretion and freshly dissected skin fragments. Ei
Publicado em: 2007
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40. Recobrimento de aço inoxidável ABNT 409 empregando filme fino bioativo obtido via sol-gel
In the present study, composites made up of ABNT 409 stainless steel was obtained where films produced according to the sol-gel route were deposited using the immersion method. A film of the binary system SiO2-CaO, and another one of the ternary system SiO2-CaOP2O5, were employed. The structure and properties of these films were characterized through the sca
Publicado em: 2007
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41. Expressão gênica diferencial de fibroblastos de linfonodos comprometidos ou não comprometidos de pacientes com câncer de mama após cultura isolada ou co-cultura com células epiteliais mamárias normais ou malignas / Differential gene expression of fibroblasts from involved or uninvolved lymph nodes from breast cancer patients cultured alone or cocultured with normal or malignant mammary epithelial cells
Tumor development may be influenced by epithelial-mesenchimal interactions in the primary site as well as in the involved lymph node from breast cancer patients. Our aim was to evaluate the proliferation rate and gene profile of fibroblasts obtained from involved and uninvolved lymph nodes from breast câncer patients and to determine the influence of normal
Publicado em: 2007
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42. The comparison of two methods to obtain human oral keratinocytes in primary culture / "Comparação de dois métodos de obtenção celular para cultura primária de queratinócitos bucais humanos"
The therapeutic procedures frequently used in oral treatments for the pathological diseases are surgical, resulting in failures of the mucosal continuity.The possibility to obtain transplantable oral epithelia from an in vitro cell culture opens new utilization perspectives not only to where it comes from, but also as a reconstructive matherial for other par
Publicado em: 2006
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43. Rotaviruses electropherotyes in diarrhoeal faeces and after isolation in MA104 cell cultures / Perfil eletroforético de rotavírus em amostras fecais diarréicas e após isolamento em cultura de células da linhagem MA104
In the present work, we have analyzed the electrophoretic profile of 15 samples of rotavírus, 11 of calves, 02 of pigs and 02 of children, that have been cultivated on MA,104 cell up to the sixth passage. The polyacrilamide gel electrophoresis(PAGE) was used to confirm the viral isolation. The aim of the work was to compare the electropherotic profile of fe
Publicado em: 2006
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44. Light and sucrose in micropropagation of Cattleya walkeriana: Anatomic and Physiologic changes / Luz e sacarose na microporapgação de Cattleya walkeriana: alteraçõies anatômicas e fisiológicas
Cattleya walkeriana ia a orchid of great decorative value. The use of micropropagation techniques are important issues in the reproduction of that specie. The use of conventional techniques of micropropagation causes plantlet losses during the acclimatization process and also promotes a high energy consumption in growth rooms which finishes by increasing the
Publicado em: 2006
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45. Caracterização de anticorpos monoclonais contra rotavírus bovino e suas aplicações como ferramenta de diagnóstico. / Characterization of monoclonal antibodies against bovine rotavirus and their diagnostic applications.
Rotavirus, a member of the Reoviridae family, is the most important etiologic agent of diarrheas in bovine. Complete particules measure 70 nm in diameter, are nonenveloped and have a distinctive double-layered icosahedral protein capsid that consists of an outer and an inner layer. Within the inner capsid is a third layer, the core, that contains the virus g
Publicado em: 2005
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46. Efeito in vitro de Saccharomyces cerevisiae sobre Guignardia citricarpa, agente causal da pinta preta dos citros. / In vitro effect of Saccharomyces cerevisiae on Guignardia citricarpa, causal agent of citrus black spot.
Due to the consumers perception about the impact caused by pesticides utilization over the environment and human health, besides the acquisition of resistance for part of the phytopathogens, the society has exercised pressures that had led to the establishment of governmental politics that restrict the use of fungicides leading agriculturists and researchers
Publicado em: 2005
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47. Recombinant antigen-based immuno-slot blot method for serodiagnosis of syphilis
Three recombinant antigens of Treponema pallidum Nichols strain were fused with GST, cloned and expressed in Escherichia coli, resulting in high levels of GST-rTp47 and GST-rTp17 expression, and supplementation with arginine tRNA for the AGR codon was needed to obtain GST-rTp15 overexpression. Purified fusion protein yields were 1.9, 1.7 and 5.3 mg/l of cell
Brazilian Journal of Medical and Biological Research. Publicado em: 2004-07
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48. Desenvolvimento de novos insumos para o diagnóstico sorológico da dengue: antígenos virais nativos e recombinates. / Development of new inputs for the serological diagnosis of dengue fever: viral antigens native and recombinant.
Dengue is the major tropical arbovirosis, in terms of morbidity and mortality. Its transmitted by hematophoges mosquitos bite of the genera Aedes and it is responsible for over one hundred million cases of dengue (DF) per year of which five hundred thousand are hemorrhagic (DHF). In Brazil, the epidemiological situation is alarming, because the country repre
Publicado em: 2004