Cdna Library
Mostrando 13-24 de 2745 artigos, teses e dissertações.
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13. Identificação de genes diferencialmente expressos em feijoeiro envolvidos na resistência ao estresse hídrico / Identification of differentially expressed genes in common bean involved in drought stress resistance
O Brasil é o segundo maior produtor de feijão, sendo a espécie mais cultivada o Phaseolus vulgaris L. Entre as três possíveis safras exploradas no Brasil, aquela que gera a maior produção é a da seca. Por outro lado, como a maioria das lavouras emprega pouca tecnologia, um dos problemas desta cultura é o estresse hídrico, que leva a uma redução n
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 03/06/2011
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14. Identificação e caracterização de genes que codificam proteínas secretadas por Hemileia vastatrix na interação com o cafeeiro / Identification and characterization of genes encoding proteins secreted by Hemileia vastatrix during interaction with coffee
The coffee leaf rust caused by the biotrophic fungus Hemileia vastatrix, it is the most important fungal disease of coffee, causing productivity losses and its control increases the costs of coffee production. This study aimed to identify genes of H. vastatrix that encode secreted proteins that may function as effectors needed for the establishment of the bi
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 24/02/2011
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15. Identificação de um fator do hospedeiro, RPS5A, envolvido na interação com a proteína de movimento (MP) de geminivírus / Identification of movement protein MP-intaracting host facotrs
The movement protein MP from bipartite geminivirus (begomovirus) facilitates the cell-to-cell and long-distance transport of viral DNA in addition to affecting viral pathogenicity. To identify host factors that interact with MP, initially a cDNA library prepared from CaLCuV (Cabbage leaf curl virus)-infected Arabidopsis leaf mRNA was generated in a pEXPAD502
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 18/02/2011
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16. Métodos estatísticos aplicados à análise de dados de etiqueta de sequência expressa / Statistical methods applied to expressed sequence tag data analisys
Pesquisas de Expressed Sequence Tags (ESTs) são uma ferramenta fundamental para identificação de genes em estudos de seqüenciamento de vários organismos. Dado uma amostra preliminar de EST de uma certa biblioteca de cDNA, vários problemas estatísticos de predição podem surgir. Em particular, é de interesse calcular o número de genes, Δ(t), qu
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/02/2011
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17. Analysis of the stress-inducible transcription factor SsNAC23 in sugarcane plants
Stresses such as cold and drought can impair plant yield and induce a highly complex array of responses. Sugarcane (Saccharum spp.) is cultivated in tropical and subtropical areas and is considered a cold-sensitive plant. We previously showed that cold stress induces the expression of several genes in in vitro sugarcane plantlets. Here we characterize one of
Scientia Agricola. Publicado em: 2011-08
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18. Insights into the transcriptome of oenocytes from Aedes aegypti pupae
Oenocytes are ectodermic cells present in the fat body of several insect species and these cells are considered to be analogous to the mammalian liver, based on their role in lipid storage, metabolism and secretion. Although oenocytes were identified over a century ago, little is known about their messenger RNA expression profiles. In this study, we investig
Memórias do Instituto Oswaldo Cruz. Publicado em: 2011-05
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19. Expressed sequence tag analysis of khat (Catha edulis) provides a putative molecular biochemical basis for the biosynthesis of phenylpropylamino alkaloids
Khat (Catha edulis Forsk.) is a flowering perennial shrub cultivated for its neurostimulant properties resulting mainly from the occurrence of (S)-cathinone in young leaves. The biosynthesis of (S)-cathinone and the related phenylpropylamino alkaloids (1S,2S)-cathine and (1R,2S)-norephedrine is not well characterized in plants. We prepared a cDNA library fro
Genetics and Molecular Biology. Publicado em: 2011
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20. Differential gene expression, induced by salicylic acid and Fusarium oxysporum f. sp. lycopersici infection, in tomato.
The objective of this work was to determine the transcript profile of tomato plants (Lycopersicon esculentum Mill.), during Fusarium oxysporum f. sp. lycopersici infection and after foliar application of salicylic acid. The suppression subtractive hybridization (SSH) technique was used to generate a cDNA library enriched for transcripts differentially expr
Pesquisa Agropecuária Brasileira. Publicado em: 2011
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21. Theobroma cacao cystatins impair Moniliophthora perniciosa mycelial growth and are involved in postponing cell death symptoms.
Three cystatin open reading frames named TcCys1, TcCys2 and TcCys3 were identified in cDNA libraries from compatible interactions between Theobroma cacao (cacao) and Moniliophthora perniciosa. In addition, an ORF named TcCys4 was identified in the cDNA library of the incompatible interaction. The cDNAs encoded conceptual proteins with 209, 127, 124, and 205
Planta. Publicado em: 2011
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22. ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds.
We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of seq
Genomics Insights. Publicado em: 2011
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23. Construção e análise de uma biblioteca de cDNA de Trichoderma harzianum crescido na presença de parede celular de Fusarium solani / Construction and analisys of a cDNA library of Trichoderma harzianum grown in presence of Fusarium solani cell wall
Espécies do gênero Trichoderma são comercialmente aplicadas como agentes de controle biológico contra fungos patógenos de plantas. Sua ação é baseada em diferentes mecanismos como a produção de antibióticos voláteis e não-voláteis, competição por espaço e nutrientes e micoparasitismo. O mecanismo de biocontrole da interação entre Trichoder
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/02/2010
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24. Análises moleculares das vias de sinalização de morte celular originadas no retículo endoplasmático / Molecular analyses of cell death pathways signalling originated in the endoplasmic reticulum
Abiotic and biotic stresses are responsible for major losses in agricultural productivity world wise. Therefore, the understanding of mechanisms of plant stress response and adaptation to such conditions should provide the foundation for engineering plant stress tolerance and hence maximizing the yield of crops growing under adverse environmental conditions.
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 11/02/2010