ESTs from seeds to assist the selective breeding of Jatropha curcas L. for oil and active compounds.
AUTOR(ES)
GOMES, K. A.
FONTE
Genomics Insights
DATA DE PUBLICAÇÃO
2011
RESUMO
We report here on the characterization of a cDNA library from seeds of Jatropha curcas L. at three stages of fruit maturation before yellowing. We sequenced a total of 2200 clones and obtained a set of 931 non-redundant sequences (unigenes) after trimming and quality control, ie, 140 contigs and 791 singlets with PHRED quality $10. We found low levels of sequence redundancy and extensive metabolic coverage by homology comparison to GO. After comparison of 5841 non-redundant ESTs from a total of 13193 reads from GenBank with KEGG, we identified tags with nucleotide variations among J. curcas accessions for genes of fatty acid, terpene, alkaloid, quinone and hormone pathways of biosynthesis. More specifically, the expression level of four genes (palmitoyl-acyl carrier protein thioesterase, 3-ketoacyl-CoA thiolase B, lysophosphatidic acid acyltransferase and geranyl pyrophosphate synthase) measured by real- time PCR proved to be significantly different between leaves and fruits. Since the nucleotide polymorphism of these tags is associated to higher level of gene expression in fruits compared to leaves, we propose this approach to speed up the search for quantitative traits in selective breeding of J. curcas. We also discuss its potential utility for the selective breeding of economically important traits in J. curcas.
ASSUNTO(S)
biofuel jatropha curcas genomics fatty acids terpenes alkaloids pinhão manso biocombustivel melhoramento genetico vegatal
ACESSO AO ARTIGO
http://www.alice.cnptia.embrapa.br/handle/doc/865928Documentos Relacionados
- Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds
- Diversity between Jatropha curcas L. accessions based on oil traits and X-ray digital images analysis from it seeds
- Continuous catalyst-free production of esters from Jatropha curcas L. oil under supercritical ethanol
- Lipase-catalyzed ethanolysis of Jatropha curcas L. oil assisted by ultrasonication
- Tipo de secagem e armazenamento na qualidade de sementes de Jatropha curcas L.
