Cdna Library
Mostrando 1-12 de 2745 artigos, teses e dissertações.
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1. Cloning and purification of the first termicin-like peptide from the cockroach Eupolyphaga sinensis
Abstract Background Termicin is an antimicrobial peptide with six cysteines forming three disulfide bridges that was firstly isolated from the salivary glands and hemocytes of the termite Pseudacanthotermes spiniger. In contrast to many broad-spectrum antimicrobial peptides, termicin is most active against filamentous fungi. Although more than one hundred
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 19/02/2016
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2. A comparison between the recombinant expression and chemical synthesis of a short cysteine-rich insecticidal spider peptide
Abstract Background: The choice between heterologous expression versus chemical synthesis for synthesizing short cysteine-rich insecticidal peptides from arthropods may impact the obtainment of yields and well-folded bioactive molecules for scientific research. Therefore, two recombinant expression systems were compared to
J. Venom. Anim. Toxins incl. Trop. Dis. Publicado em: 11/08/2015
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3. The consensus sequence of FAMLF alternative splice variants is overexpressed in undifferentiated hematopoietic cells
The familial acute myeloid leukemia related factor gene (FAMLF) was previously identified from a familial AML subtractive cDNA library and shown to undergo alternative splicing. This study used real-time quantitative PCR to investigate the expression of the FAMLF alternative-splicing transcript consensus sequence (FAMLF-CS) in peripheral blood mononuclear ce
Braz J Med Biol Res. Publicado em: 12/06/2015
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4. Expression of stress-related genes in zebrawood (Astronium fraxinifolium, Anacardiaceae) seedlings following germination in microgravity
Seeds of a tropical tree species from Brazil, Astronium fraxinifolium, or zebrawood, were germinated, for the first time in microgravity, aboard the International Space Station for nine days. Following three days of subsequent growth under normal terrestrial gravitational conditions, greater root length and numbers of secondary roots was observed in the micr
Genet. Mol. Biol.. Publicado em: 2014
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5. Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus.Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells.
Mem. Inst. Oswaldo Cruz. Publicado em: 10/10/2013
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6. A directed approach for the identification of transcripts harbouring the spliced leader sequence and the effect of trans-splicing knockdown in Schistosoma mansoni
Schistosomiasis is a major neglected tropical disease caused by trematodes from the genus Schistosoma. Because schistosomes exhibit a complex life cycle and numerous mechanisms for regulating gene expression, it is believed that spliced leader (SL) trans-splicing could play an important role in the biology of these parasites. The purpose of this study was to
Mem. Inst. Oswaldo Cruz. Publicado em: 2013-09
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7. Effects of BST and high energy diet on gene expression in mammary parenchyma of dairy heifers
The objective of this study was to determine the effects of dietary energy and recombinant bovine somatotropin (bST) injection to identify genes that might control mammogenesis. Total RNA was extracted from the parenchymal tissue of 32 heifers randomly assigned to one of four treatments: two diets (a standard diet and a high energy, high protein diet), each
R. Bras. Zootec.. Publicado em: 2013-07
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8. Insights into the Melipona scutellaris (Hymenoptera, Apidae, Meliponini) fat body transcriptome
The insect fat body is a multifunctional organ analogous to the vertebrate liver. The fat body is involved in the metabolism of juvenile hormone, regulation of environmental stress, production of immunity regulator-like proteins in cells and protein storage. However, very little is known about the molecular mechanisms involved in fat body physiology in sting
Genet. Mol. Biol.. Publicado em: 2013
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9. Clonagem e estudos de expressão de enzimas do fungo filamentoso Trichoderma harzianum IOC-3844 envolvidas na degradação de biomassa
The plant biomass is a large-scale available resource and one of its more important applications is the second-generation ethanol production. However, the enzyme cost is one of the biggest barriers for economically viable ethanol from biomass. Therefore, it is important to identify fungal strains that can produce high concentrations of plant biomass-degradin
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 06/07/2012
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10. Caracterização funcional e estrutural de uma nova fosfolipase A2 ácida de Bothrops moojeni / Functional and structural characterization of a new acidic phospholipase A2 from Bothrops moojeni
The phospholipase A2 (PLA2s) are enzymes that induce various pharmacological effects and usually correspond to a higher percentage of the protein content of snake venoms. Thus, isolation, biochemical, functional and structural characterization of PLA2s may generate important information for a better understanding of the pharmacological effects and toxicity i
IBICT - Instituto Brasileiro de Informação em Ciência e Tecnologia. Publicado em: 26/01/2012
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11. Isolation and in silico characterization of cDNA encoding cyclophilin from etiolated Vigna mungo seedlings
A full-length cDNA clone encoding cyclophilin gene of 848 bp, including a 519 bp open reading frame, has been isolated from the cDNA library constructed from etiolated seedlings of Vigna mungo (GenBank FN668732). The cDNA sequence showed 97% identity with Vigna radiata cyclophilin mRNA. The sequence was GC rich and lacked introns. The open reading frame enco
Braz. J. Plant Physiol.. Publicado em: 2012
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12. A novel lipocalin homologue from the venom gland of Deinagkistrodon acutus similar to mammalian lipocalins
Lipocalins are involved in a variety of functions including retinol transport, cryptic coloration, olfaction, pheromone transport, prostaglandin synthesis, regulation of the immune response and cell homeostatic mediation. A full-length cDNA clone (named d-lipo), isolated from the venom gland cDNA library of Deinagkistrodon acutus, contained an insert of 664
Journal of Venomous Animals and Toxins including Tropical Diseases. Publicado em: 2012